Summary of 258-Oginuma-EmbryoDev

SSBD:database URL
Time-lapse imaging of hba reporter expression in the GFP knock-in N. furzeri embryos.
Relase date
Updated date
Image data based on Experiment
Number of Datasets
1 ( Image datasets: 1, Quantitative data datasets: 0 )
Size of Datasets
50.0 MB ( Image datasets: 50.0 MB, Quantitative data datasets: 0 bytes )

Nothobranchius furzeri
Gene symbol(s)
Protein tag(s)

Molecular Function (MF)
Biological Process (BP)
CRISPR-cas system
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
X scale
2.468 micrometer/pixel
Y scale
2.468 micrometer/pixel
Z scale
T scale
0.14 sceonds of time interval

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Related paper(s)

Masayuki Oginuma, Moana Nishida, Tomomi Ohmura-Adachi, Kota Abe, Shohei Ogamino, Chihiro Mogi, Hideaki Matsui, Tohru Ishitani (2022) Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate aging., Scientific reports, Volume 12, Number 1, pp. 11628

Published in 2022 Jul 8 (Electronic publication in July 8, 2022, midnight )

(Abstract) The African turquoise killifish Nothobranchius furzeri (N. furzeri) is a useful model organism for studying aging, age-related diseases, and embryonic diapause. CRISPR/Cas9-mediated gene knockout and Tol2 transposon-mediated transgenesis in N. furzeri have been reported previously. However, these methods take time to generate knockout and transgenic fish. In addition, knock-in technology that inserts large DNA fragments as fluorescent reporter constructs into the target gene in N. furzeri has not yet been established. Here, we show that triple-target CRISPR-mediated single gene disruption efficiently produces whole-body biallelic knockout and enables the examination of gene function in the F0 generation. In addition, we developed a method for creating the knock-in reporter N. furzeri without crossing by optimizing the CRISPR/Cas9 system. These methods drastically reduce the duration of experiments, and we think that these advances will accelerate aging and developmental studies using N. furzeri.
(MeSH Terms)

Tohru Ishitani , Osaka University , Research Institute for Microbial Diseases , Division of Cellular and Molecular Biology, Department of Homeostatic Regulation

Dataset List of 258-Oginuma-EmbryoDev

Dataset ID
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# 10674
Dataset Kind Image data
Dataset Size 50.0 MB
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