Summary of 258-Oginuma-EmbryoDev

SSBD:database
SSBD:database URL
Title
Time-lapse imaging of hba reporter expression in the GFP knock-in N. furzeri embryos.
Description
-
Relase date
2023-05-11
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
1 ( Image datasets: 1, Quantitative data datasets: 0 )
Size of Datasets
50.0 MB ( Image datasets: 50.0 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Nothobranchius furzeri
Gene symbol(s)
hba
Protein name(s)
NA
Protein tag(s)
GFP

Datatype
-
Molecular Function (MF)
Biological Process (BP)
CRISPR-cas system
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
X scale
2.468 micrometer/pixel
Y scale
2.468 micrometer/pixel
Z scale
-
T scale
0.14 sceonds of time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Masayuki Oginuma, Moana Nishida, Tomomi Ohmura-Adachi, Kota Abe, Shohei Ogamino, Chihiro Mogi, Hideaki Matsui, Tohru Ishitani (2022) Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate aging., Scientific reports, Volume 12, Number 1, pp. 11628

Published in 2022 Jul 8 (Electronic publication in July 8, 2022, midnight )

(Abstract) The African turquoise killifish Nothobranchius furzeri (N. furzeri) is a useful model organism for studying aging, age-related diseases, and embryonic diapause. CRISPR/Cas9-mediated gene knockout and Tol2 transposon-mediated transgenesis in N. furzeri have been reported previously. However, these methods take time to generate knockout and transgenic fish. In addition, knock-in technology that inserts large DNA fragments as fluorescent reporter constructs into the target gene in N. furzeri has not yet been established. Here, we show that triple-target CRISPR-mediated single gene disruption efficiently produces whole-body biallelic knockout and enables the examination of gene function in the F0 generation. In addition, we developed a method for creating the knock-in reporter N. furzeri without crossing by optimizing the CRISPR/Cas9 system. These methods drastically reduce the duration of experiments, and we think that these advances will accelerate aging and developmental studies using N. furzeri.
(MeSH Terms)

Contact
Tohru Ishitani , Osaka University , Research Institute for Microbial Diseases , Division of Cellular and Molecular Biology, Department of Homeostatic Regulation
Contributors


Dataset List of 258-Oginuma-EmbryoDev

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 10674
Dataset Kind Image data
Dataset Size 50.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data