Takafumi Miyamoto, Song-Iee Han, Hitoshi Shimano (2021) Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)., STAR protocols, Volume 2, Number 3, pp. 100721
Published in 2021 Sep 17 (Electronic publication in Aug. 5, 2021, midnight )
(Abstract) Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach. For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021.(MeSH Terms)