Summary of 229-Yamada-GeneExpression

SSBD:database
SSBD:database URL
Title
Time-lapse images of light inducible PA-Gal4cc activity in mouse brain or HEK293T cells
Description
-
Relase date
2022-11-23
Updated date
-
License
CC BY-NC
Kind
Image data based on Experiment
Number of Datasets
4 ( Image datasets: 4, Quantitative data datasets: 0 )
Size of Datasets
1.8 GB ( Image datasets: 1.8 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus, Homo sapiens
Cell lines(s)
HEK293T
Gene symbol(s)
NA
Protein name(s)
NA
Reporter(s)
PA-Gal4cc

Datatype
-
Molecular Function (MF)
translation activator activity
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
X scale
0.75 micrometer/pixel
Y scale
0.75 micrometer/pixel
Z scale
-
T scale
5 minutes per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Mayumi Yamada, Shinji C Nagasaki, Yusuke Suzuki, Yukinori Hirano, Itaru Imayoshi (2020) Optimization of Light-Inducible Gal4/UAS Gene Expression System in Mammalian Cells., iScience, Volume 23, Number 9, pp. 101506

Published in 2020 Aug 27 (Electronic publication in Aug. 27, 2020, midnight )

(Abstract) Light-inducible gene expression systems represent powerful methods for studying the functional roles of dynamic gene expression. Here, we developed an optimized light-inducible Gal4/UAS gene expression system for mammalian cells. We designed photoactivatable (PA)-Gal4 transcriptional activators based on the concept of split transcription factors, in which light-dependent interactions between Cry2-CIB1 PA-protein interaction modules can reconstitute a split Gal4 DNA-binding domain and p65 transcription activation domain. We developed a set of PA-Gal4 transcriptional activators (PA-Gal4cc), which differ in terms of induced gene expression levels following pulsed or prolonged light exposure, and which have different activation/deactivation kinetics. These systems offer optogenetic tools for the precise manipulation of gene expression at fine spatiotemporal resolution in mammalian cells.

Contact
Itaru Imayoshi , kyoto University , Graduate School of Biostudies , Research Center for Dynamic Living Systems
Contributors


Dataset List of 229-Yamada-GeneExpression

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 8726
Dataset Kind Image data
Dataset Size 566.0 MB
4D view
SSBD:OMERO
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Download Image data

# 8727
Dataset Kind Image data
Dataset Size 262.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 8728
Dataset Kind Image data
Dataset Size 461.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 8729
Dataset Kind Image data
Dataset Size 560.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data