Summary of 194-Obashi-SpineDyn

SSBD:database
SSBD:database URL
Title
Microscopy images or BDML file for quantiative information about actin polymers mobility in mouse dendritic spines.
Description
-
Relase date
2022-03-31
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
21 ( Image datasets: 21, Quantitative data datasets: 0 )
Size of Datasets
9.4 MB ( Image datasets: 9.4 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Gene symbol(s)
NA
Protein name(s)
ADF, Tiam1QK, cofilin
Protein tag(s)
EGFP, EGFP5, mCherry, mRFP

Datatype
-
Molecular Function (MF)
NA, signaling molecule
Biological Process (BP)
actin polymerization, stimulation of synapse structural plasticity
Cellular Component (CC)
dendritic spine
Biological Imaging Method
time lapse microscopy, two-photon laser scanning microscopy, structured illumination microscopy
X scale
0.206 micrometer/pixel, 0.138 micrometer/pixel, 0.103 micrometer/pixel, 0.032 micrometer/pixel, 0.069 micrometer/pixel
Y scale
0.206 micrometer/pixel, 0.138 micrometer/pixel, 0.103 micrometer/pixel, 0.032 micrometer/pixel, 0.069 micrometer/pixel
Z scale
-
T scale
-11, 1, 5, 15, 30 minutes, -10, 1, 15, 30 minutes, single time point (C1, C2, C1 mask, C2 mask), -20, 1, 10, 30 minutes, -20, -10, -1, 1, 15, 30 minutes

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Kazuki Obashi, Atsushi Matsuda, Yasuhiro Inoue, Shigeo Okabe (2019) Precise Temporal Regulation of Molecular Diffusion within Dendritic Spines by Actin Polymers during Structural Plasticity., Cell reports, Volume 27, Number 5, pp. 1503-1515.e8

Published in 2019 Apr 30

(Abstract) The biochemical transduction of excitatory synaptic signals occurs in the cytoplasm within dendritic spines. The associated reaction kinetics are shaped by the mobility of the signaling molecules; however, accurate monitoring of diffusional events within the femtoliter-sized spine structures has not yet been demonstrated. Here, we applied two-photon fluorescence correlation spectroscopy and raster image correlation spectroscopy to monitor protein dynamics within spines, revealing that F-actin restricts the mobility of proteins with a molecular mass of >100 kDa. This restriction is transiently removed during actin remodeling at the initial phase of spine structural plasticity. Photobleaching experiments combined with super-resolution imaging indicate that this increase in mobility facilitates molecular interactions, which may modulate the functions of key postsynaptic signaling molecules, such as Tiam1 and CaMKII. Thus, actin polymers in dendritic spines act as precise temporal regulators of molecular diffusion and modulate signal transduction during synaptic plasticity.
(MeSH Terms)

Contact
Shigeo Okabe , the University of Tokyo, Tokyo , Department of Cellular Neurobiology, Graduate School of Medicine
Contributors
Kazuki Obashi


Dataset List of 194-Obashi-SpineDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 7483
Dataset Kind Image data
Dataset Size 193.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7484
Datast ID Fig3A_MNI-Glu-Plus
Dataset Kind Image data
Dataset Size 897.2 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7485
Datast ID Fig3F_control-Sh
Dataset Kind Image data
Dataset Size 769.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7486
Dataset Kind Image data
Dataset Size 193.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7487
Datast ID Fig3F_Sh_cofilin
Dataset Kind Image data
Dataset Size 769.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7488
Datast ID Fig4A_SIM_sLTP
Dataset Kind Image data
Dataset Size 2.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7489
Datast ID Fig5A_CaMK_EGFP_C1
Dataset Kind Image data
Dataset Size 161.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7490
Datast ID Fig5A_CaMK_EGFP_C2
Dataset Kind Image data
Dataset Size 161.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7491
Dataset Kind Image data
Dataset Size 193.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7492
Dataset Kind Image data
Dataset Size 193.3 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7493
Dataset Kind Image data
Dataset Size 161.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7494
Dataset Kind Image data
Dataset Size 161.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7495
Datast ID Fig5A_Tiam_EGFP_C1
Dataset Kind Image data
Dataset Size 769.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7496
Datast ID Fig5A_Tiam_EGFP_C2
Dataset Kind Image data
Dataset Size 769.1 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7497
Dataset Kind Image data
Dataset Size 769.3 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7498
Dataset Kind Image data
Dataset Size 769.3 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7499
Dataset Kind Image data
Dataset Size 98.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7500
Dataset Kind Image data
Dataset Size 98.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7501
Dataset Kind Image data
Dataset Size 98.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7502
Dataset Kind Image data
Dataset Size 98.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7503
Dataset Kind Image data
Dataset Size 98.5 KB
4D view
SSBD:OMERO
Download BDML
Download Image data