Detail of Fig6A_EGFP_after_stimulus
Project
Title
2D SIM image of glutamate-uncaging-induced spine neurons expressing EGFP and mRFP at 13–19 DIV.
Description
2D SIM image of glutamate-uncaging-induced spine neurons expressing EGFP at 13–19 DIV.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.tif
Data size
98.5 KB
Protein tags
EGFP, mRFP
Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
actin polymerization
(
GO:0030041
)
Cellular Component (CC)
dendritic spine
(
GO:0043197
)
Biological Imaging Method
structured illumination microscopy
(
Fbbi:00000332
)
X scale
0.032 micrometer/pixel
Y scale
0.032 micrometer/pixel
Z scale
-
T scale
single time point (C1, C2, C1 mask, C2 mask)
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Obashi K, et. al. (2019) Cell Rep., 27(5):1503-1515.
Related paper(s)
Kazuki Obashi, Atsushi Matsuda, Yasuhiro Inoue, Shigeo Okabe (2019) Precise Temporal Regulation of Molecular Diffusion within Dendritic Spines by Actin Polymers during Structural Plasticity., Cell reports, Volume 27, Number 5, pp. 1503-1515.e8
Published in 2019 Apr 30
- doi: 10.1016/j.celrep.2019.04.006
-
pmid: 31042476
(Abstract) The biochemical transduction of excitatory synaptic signals occurs in the cytoplasm within dendritic spines. The associated reaction kinetics are shaped by the mobility of the signaling molecules; however, accurate monitoring of diffusional events within the femtoliter-sized spine structures has not yet been demonstrated. Here, we applied two-photon fluorescence correlation spectroscopy and raster image correlation spectroscopy to monitor protein dynamics within spines, revealing that F-actin restricts the mobility of proteins with a molecular mass of >100 kDa. This restriction is transiently removed during actin remodeling at the initial phase of spine structural plasticity. Photobleaching experiments combined with super-resolution imaging indicate that this increase in mobility facilitates molecular interactions, which may modulate the functions of key postsynaptic signaling molecules, such as Tiam1 and CaMKII. Thus, actin polymers in dendritic spines act as precise temporal regulators of molecular diffusion and modulate signal transduction during synaptic plasticity.(MeSH Terms)
- Actin Cytoskeleton/metabolism[major]
- Actins/metabolism[major]
- Animals
- Biological Transport
- Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
- Cells, Cultured
- Dendritic Spines/metabolism[major]
- Dendritic Spines/ultrastructure
- Diffusion
- Female
- Male
- Mice
- Mice, Inbred ICR
- Neuronal Plasticity[major]
- Signal Transduction
- T-Lymphoma Invasion and Metastasis-inducing Protein 1/metabolism
Contact
Shigeo Okabe
, the University of Tokyo, Tokyo
, Department of Cellular Neurobiology, Graduate School of Medicine
Contributors
Kazuki Obashi
OMERO Dataset
OMERO Project
Source