Summary of 189-Kobayashi-NSCsDyn

SSBD:database
SSBD:database URL
Title
Confocal microscopy images of lysosomes situation in NSCs in both the DG and SVZ of mice.
Description
-
Relase date
2022-03-31
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
17 ( Image datasets: 17, Quantitative data datasets: 0 )
Size of Datasets
6.8 GB ( Image datasets: 6.8 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Gene symbol(s)
CtsB, CtsL, Lamp1
Protein name(s)
GFAP, Nestin, TFEB, caTFEB
Protein tag(s)
GFP, mCherry

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
cell division, negative regulation of lysosomal protein catabolic process
Cellular Component (CC)
lysosome, NA
Biological Imaging Method
confocal microscopy
X scale
0.472329 micrometer/pixel, 0.5313701 micrometer/pixel, 0.4723290 micrometer/pixel, 0.4612588 micrometer/pixel, 0.4612 micrometer/pixel, 0.0885617 micrometer/pixel, 0.1107021 micrometer/pixel, 0.3148860 micrometer/pixel, 0.6918882 micrometer/pixel, 0.0948875 micrometer/pixel, 0.0708494 micrometer/pixel, 0.3936075 micrometer/pixel, 0.922 micrometer/pixel, 0.9225176 micrometer/pixel, 0.0878588 micrometer/pixel
Y scale
0.472329 micrometer/pixel, 0.5313701 micrometer/pixel, 0.4723290 micrometer/pixel, 0.4612588 micrometer/pixel, 0.4612 micrometer/pixel, 0.0885617 micrometer/pixel, 0.1107021 micrometer/pixel, 0.3148860 micrometer/pixel, 0.6918882 micrometer/pixel, 0.0948875 micrometer/pixel, 0.0708494 micrometer/pixel, 0.3936075 micrometer/pixel, 0.922 micrometer/pixel, 0.9225176 micrometer/pixel, 0.0878588 micrometer/pixel
Z scale
2.4 micrometer/slice, 1.6 micrometer/slice, 1 micrometer/slice
T scale
10 minutes per time interval, NA

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Taeko Kobayashi, Wenhui Piao, Toshiya Takamura, Hiroshi Kori, Hitoshi Miyachi, Satsuki Kitano, Yumiko Iwamoto, Mayumi Yamada, Itaru Imayoshi, Seiji Shioda, Andrea Ballabio, Ryoichiro Kageyama (2019) Enhanced lysosomal degradation maintains the quiescent state of neural stem cells., Nature communications, Volume 10, Number 1, pp. 5446

Published in 2019 Nov 29 (Electronic publication in Nov. 29, 2019, midnight )

(Abstract) Quiescence is important for sustaining neural stem cells (NSCs) in the adult brain over the lifespan. Lysosomes are digestive organelles that degrade membrane receptors after they undergo endolysosomal membrane trafficking. Enlarged lysosomes are present in quiescent NSCs (qNSCs) in the subventricular zone of the mouse brain, but it remains largely unknown how lysosomal function is involved in the quiescence. Here we show that qNSCs exhibit higher lysosomal activity and degrade activated EGF receptor by endolysosomal degradation more rapidly than proliferating NSCs. Chemical inhibition of lysosomal degradation in qNSCs prevents degradation of signaling receptors resulting in exit from quiescence. Furthermore, conditional knockout of TFEB, a lysosomal master regulator, delays NSCs quiescence in vitro and increases NSC proliferation in the dentate gyrus of mice. Taken together, our results demonstrate that enhanced lysosomal degradation is an important regulator of qNSC maintenance.
(MeSH Terms)

Contact
Taeko Kobayashi, Ryoichiro Kageyama , Kyoto University, Kyoto University , Institute for Frontier Life and Medical Sciences, Institute for Frontier Life and Medical Sciences
Contributors


Dataset List of 189-Kobayashi-NSCsDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
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# 7402
Datast ID SuppFig4a_SVZ4_10z
Dataset Kind Image data
Dataset Size 8.2 MB
4D view
SSBD:OMERO
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# 7403
Datast ID SuppFig4a_SVZ4_20z
Dataset Kind Image data
Dataset Size 64.4 MB
4D view
SSBD:OMERO
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# 7404
Datast ID SuppFig4a_SVZ4_40z
Dataset Kind Image data
Dataset Size 328.5 MB
4D view
SSBD:OMERO
Download BDML
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# 7405
Dataset Kind Image data
Dataset Size 15.7 MB
4D view
SSBD:OMERO
Download BDML
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# 7406
Dataset Kind Image data
Dataset Size 75.3 MB
4D view
SSBD:OMERO
Download BDML
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# 7407
Dataset Kind Image data
Dataset Size 262.8 MB
4D view
SSBD:OMERO
Download BDML
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# 7408
Dataset Kind Image data
Dataset Size 15.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7409
Dataset Kind Image data
Dataset Size 94.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7410
Dataset Kind Image data
Dataset Size 262.8 MB
4D view
SSBD:OMERO
Download BDML
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# 7411
Dataset Kind Image data
Dataset Size 15.8 MB
4D view
SSBD:OMERO
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# 7412
Dataset Kind Image data
Dataset Size 94.1 MB
4D view
SSBD:OMERO
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# 7413
Dataset Kind Image data
Dataset Size 328.4 MB
4D view
SSBD:OMERO
Download BDML
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# 7414
Datast ID SuppFig4e_DG_10z
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 7415
Datast ID SuppFig4e_DG_20z
Dataset Kind Image data
Dataset Size 38.6 MB
4D view
SSBD:OMERO
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# 7416
Datast ID SuppFig4e_DG_60z
Dataset Kind Image data
Dataset Size 144.4 MB
4D view
SSBD:OMERO
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# 7417
Datast ID Supp_movie1_wt_24h
Dataset Kind Image data
Dataset Size 2.5 GB
4D view
SSBD:OMERO
Download BDML
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# 7418
Datast ID Supp_movie2_ca_24h
Dataset Kind Image data
Dataset Size 2.5 GB
4D view
SSBD:OMERO
Download BDML
Download Image data