Summary of 186-Tsuboi-TumorDyn

SSBD:database
SSBD:database URL
Title
Images of tumor-bearing mice or KPL-4 cells apoptosis treated with Kadcyla for different time.
Description
-
Relase date
2022-11-23
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
29 ( Image datasets: 29, Quantitative data datasets: 0 )
Size of Datasets
86.4 MB ( Image datasets: 86.4 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens, Mus musculus
Cell lines(s)
KPL-4
Gene symbol(s)
NA
Protein name(s)
HER2
Reporter(s)
ICG- EGFP-Annexin, ICG-EGFP, ICG-mPlum

Datatype
-
Molecular Function (MF)
Biological Process (BP)
apoptosis
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy
X scale
264.58 micrometer/pixel
Y scale
264.58 micrometer/pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Setsuko Tsuboi, Takashi Jin (2020) Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo., RSC advances, Volume 10, Number 63, pp. 38244-38250

Published in 2020 Oct 15 (Electronic publication in Oct. 16, 2020, midnight )

(Abstract) Indocyanine green (ICG) labelled recombinant annexin V proteins (ICG-EGFP-Annexin V and ICG-mPlum-Annexin V) were synthesized for dual-colour fluorescence imaging of tumour cell apoptosis in vitro and in vivo. The ICG-labelled fluorescent annexin V proteins showed dual (near-infrared and visible) fluorescence emissions with binding ability to phosphatidylserines on the plasma membranes of apoptotic cells. Although several types of fluorescence labelled annexin V (e.g. FITC-annexin V, Cy3- and Cy5-annexin V) have been reported, there are no dual-colour (near-infrared/visible) emitting apoptosis-detection probes which can be used in vitro and in vivo. In this paper, the utilities of the dual-colour fluorescent annexin V are demonstrated for in vitro and in vivo fluorescence imaging of the apoptosis of human breast tumour cells induced by an antibody-drug conjugate, Kadcyla. The results suggest that the present annexin V probes will be useful to visualize the action of anti-cancer drugs against tumours both at the cellular and whole-body level.

Contact
Takashi Jin , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Developmental Dynamics
Contributors


Dataset List of 186-Tsuboi-TumorDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 7683
Dataset Kind Image data
Dataset Size 5.4 MB
4D view
SSBD:OMERO
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# 7684
Dataset Kind Image data
Dataset Size 5.4 MB
4D view
SSBD:OMERO
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# 7685
Dataset Kind Image data
Dataset Size 5.3 MB
4D view
SSBD:OMERO
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# 7686
Dataset Kind Image data
Dataset Size 5.3 MB
4D view
SSBD:OMERO
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# 7687
Datast ID FigS5a_24h_0.1nm
Dataset Kind Image data
Dataset Size 1.2 MB
4D view
SSBD:OMERO
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# 7688
Datast ID FigS5a_24h_0.01nm
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
Download BDML
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# 7689
Datast ID FigS5a_24h_0nm
Dataset Kind Image data
Dataset Size 1.3 MB
4D view
SSBD:OMERO
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# 7690
Datast ID FigS5a_24h_1nm
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
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# 7691
Datast ID FigS5a_24h_10nm
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
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# 7692
Datast ID FigS5a_24h_100nm
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
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# 7693
Datast ID FigS5a_48h_0.1nm
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
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# 7694
Datast ID FigS5a_48h_0.01nm
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
Download BDML
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# 7695
Datast ID FigS5a_48h_0nm
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
Download BDML
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# 7696
Datast ID FigS5a_48h_1nm
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7697
Datast ID FigS5a_48h_10nm
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7698
Datast ID FigS5a_48h_100nm
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7699
Datast ID FigS5a_72h_0.1nm
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
SSBD:OMERO
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# 7700
Datast ID FigS5a_72h_0.01nm
Dataset Kind Image data
Dataset Size 1.4 MB
4D view
SSBD:OMERO
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# 7701
Datast ID FigS5a_72h_0nm
Dataset Kind Image data
Dataset Size 1.4 MB
4D view
SSBD:OMERO
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# 7702
Datast ID FigS5a_72h_1nm
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7703
Datast ID FigS5a_72h_10nm
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7704
Datast ID FigS5a_72h_100nm
Dataset Kind Image data
Dataset Size 1.3 MB
4D view
SSBD:OMERO
Download BDML
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# 7705
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
Download BDML
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# 7706
Dataset Kind Image data
Dataset Size 5.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7707
Dataset Kind Image data
Dataset Size 5.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 7708
Dataset Kind Image data
Dataset Size 5.3 MB
4D view
SSBD:OMERO
Download BDML
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# 7709
Dataset Kind Image data
Dataset Size 5.3 MB
4D view
SSBD:OMERO
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# 7710
Dataset Kind Image data
Dataset Size 5.4 MB
4D view
SSBD:OMERO
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# 7711
Dataset Kind Image data
Dataset Size 5.3 MB
4D view
SSBD:OMERO
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