SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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128-Muta-IECsERKDyn

https://ssbd.riken.jp/database/128-Muta-IECsERKDyn/

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ssbd-repos-000128

https://ssbd.riken.jp/repository/128/

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Fluorescence images and BDML file for quantitative information about mouse intestinal epithelial cells (IECs) extracellular signal-regulated kinase (ERK) activity dynamics

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2021-09-30

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CC BY

Image data based on Experiment

470 ( Image datasets: 470, Quantitative data datasets: 0 )

62.5 GB ( Image datasets: 62.5 GB, Quantitative data datasets: 0 bytes )

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Mus musculus

Egfl6, ErbB2, Flna, Lrig3, Noggin, Troy, Wnt-3a, Wnt3a

E-cadherin, EGFR, ERK

Fucci2a, anti-E-cadherin, anti-EGFR

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ERBB2-EGFR signaling pathway, ERBB2 signaling pathway, epidermal growth factor signaling pathway, activation of MAPERK kinase

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FRET, time lapse microscopy, fluorescence microscopy

0.662 micrometer/pixel, 0.31 micrometer/pixel, 0.828 micrometer/pixel, 0.497 micrometer/pixel, 0.621 micrometer/pixel, 0.414 micrometer/pixel, 0.552 micrometer/pixel

0.662 micrometer/pixel, 0.31 micrometer/pixel, 0.828 micrometer/pixel, 0.497 micrometer/pixel, 0.621 micrometer/pixel, 0.414 micrometer/pixel, 0.552 micrometer/pixel

1 micrometer/slice, 4 micrometer/slice, 3 micrometer/slice, 2 micrometer/slice, 10 micrometer/slice, 6 micrometer/slice, 5 micrometer/slice

90.31 second for each time interval, 61.16 second for each time interval, 107.50 second for each time interval, 108.86 second for each time interval, 90.16 second for each time interval, 60.94 second for each time interval, 66.61 second for each time interval, 122.92 second for each time interval, 105.59 second for each time interval, NA, 68.34 second for each time interval, 68.8 second for each time interval, 91.41 second for each time interval, 61.47 second for each time interval, 118.01 second for each time interval, 61.53 second for each time interval, 66.18 second for each time interval, 69.70 second for each time interval, 1second for each time interval, 67.05 second for each time interval, 61.22 second for each time interval, 61.23 second for each time interval, 71.07 second for each time interval, 185.34 second for each time interval, 90.14 second for each time interval, 61.84 second for each time interval, 90.86 second for each time interval, 66.63 second for each time interval, 60.87 second for each time interval, 91.47 second for each time interval, 68.35 second for each time interval, 60.98 second for each time interval, 91.95 second for each time interval, 100.73 second for each time interval, 91.68 second for each time interval, 73.71 second for each time interval, 70.99 second for each time interval, 67.79 second for each time interval, 73.18 second for each time interval, 70.66 second for each time interval, 111.53 second for each time interval, 107.25 second for each time interval, 103.71 second for each time interval, 90.12 second for each time interval, 90.17 second for each time interval, 101.35 second for each time interval, 94.27 second for each time interval, 60.01 second for each time interval, 71.85 second for each time interval, 109.23 second for each time interval, 90.96 second for each time interval, 68.80 second for each time interval, 61.1 second for each time interval, 91.03 second for each time interval, 60.93 second for each time interval, 104.34 second for each time interval, 90.72 second for each time interval, 102.10 second for each time interval, 111.51 second for each time interval, 71.7 second for each time interval, 118.02 second for each time interval, 90.36 second for each time interval, 68.86 second for each time interval, 107.24 second for each time interval, 61.35 second for each time interval, 103.72 second for each time interval, 60.47 second for each time interval

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Yu Muta, Yoshihisa Fujita, Kenta Sumiyama, Atsuro Sakurai, M Mark Taketo, Tsutomu Chiba, Hiroshi Seno, Kazuhiro Aoki, Michiyuki Matsuda, Masamichi Imajo (2018) Composite regulation of ERK activity dynamics underlying tumour-specific traits in the intestine., Nature communications, Volume 9, Number 1, pp. 2174

Published in 2018 Jun 5 (Electronic publication in June 5, 2018, midnight )

• doi: 10.1038/s41467-018-04527-8
• pmid: 29872037
  

(Abstract) Acting downstream of many growth factors, extracellular signal-regulated kinase (ERK) plays a pivotal role in regulating cell proliferation and tumorigenesis, where its spatiotemporal dynamics, as well as its strength, determine cellular responses. Here, we uncover the ERK activity dynamics in intestinal epithelial cells (IECs) and their association with tumour characteristics. Intravital imaging identifies two distinct modes of ERK activity, sustained and pulse-like activity, in IECs. The sustained and pulse-like activities depend on ErbB2 and EGFR, respectively. Notably, activation of Wnt signalling, the earliest event in intestinal tumorigenesis, augments EGFR signalling and increases the frequency of ERK activity pulses through controlling the expression of EGFR and its regulators, rendering IECs sensitive to EGFR inhibition. Furthermore, the increased pulse frequency is correlated with increased cell proliferation. Thus, ERK activity dynamics are defined by composite inputs from EGFR and ErbB2 signalling in IECs and their alterations might underlie tumour-specific sensitivity to pharmacological EGFR inhibition.

(MeSH Terms)

• Animals
• Cell Culture Techniques
• Cell Transformation, Neoplastic/genetics[major]
• Epithelial Cells/metabolism[major]
• Extracellular Signal-Regulated MAP Kinases/genetics[major]
• Extracellular Signal-Regulated MAP Kinases/metabolism
• Gene Expression Profiling[major]
• Intestines/cytology[major]
• Kinetics
• MAP Kinase Signaling System/genetics
• Mice, Inbred C57BL
• Mice, Transgenic
• Microscopy, Fluorescence, Multiphoton
• Organoids/cytology
• Organoids/metabolism
• Time-Lapse Imaging/methods

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Masamichi Imajo , Kyoto University , Graduate School of Biostudies , Laboratory of Bioimaging and Cell Signaling

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