Summary of 124-Ueda-miPSRetina

SSBD:database
SSBD:database URL
Title
Immunofluorescence images of photoreceptor-specific genes expressing in mouse iPS cells derived 3D retinal
Description
-
Relase date
2019-11-20
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
31 ( Image datasets: 31, Quantitative data datasets: 0 )
Size of Datasets
99.0 MB ( Image datasets: 99.0 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Gene symbol(s)
RXRy, Rhodopsin, S-opsin, Trb2, crx
Protein name(s)
BMP4

Datatype
retina differentiation
Molecular Function (MF)
Biological Process (BP)
photoreceptor development, retinal rod cell differentiation
Cellular Component (CC)
photoreceptor cilium
Biological Imaging Method
-
XYZ Scale
XY:0.3125849 micrometer/pixel, Z: NA, XY:0.6251697 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
FluorescenceCorrelationSpectroscopy
Contrast method
Fluorescence
Microscope model
ZEISS LSM700
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Kaori Ueda, Akishi Onishi, Shin-Ichiro Ito, Makoto Nakamura, Masayo Takahashi (2018) Generation of three-dimensional retinal organoids expressing rhodopsin and S- and M-cone opsins from mouse stem cells., Biochemical and biophysical research communications, Volume 495, Number 4, pp. 2595-2601

Published in 2018 Jan 22 (Electronic publication in Dec. 20, 2017, midnight )

(Abstract) PURPOSE: Three-dimensional retinal organoids can be differentiated from embryonic stem cells/induced pluripotent stem cells (ES/iPS cells) under defined medium conditions. We modified the serum-free floating culture of embryoid body-like aggregates with quick reaggregation (SFEBq) culture procedure to obtain retinal organoids expressing more rod photoreceptors and S- and M-cone opsins. METHODS: Retinal organoids differentiated from mouse Nrl-eGFP iPS cells were cultured in various mediums during photoreceptor development. To promote rod photoreceptor development, organoids were maintained in media containing 9-cis retinoic acids (9cRA). To obtain retinal organoids with M-opsin expression, we cultured in medium with 1% fetal bovine serum (FBS) supplemented with T3, BMP4, and DAPT. Section immunohistochemistry was performed to visualize the expression of photoreceptor markers. RESULTS: In three-dimensional (3D) retinas exposed to 9cRA, rhodopsin was expressed earlier and S-cone opsins were suppressed. We could maintain 3D retinas up to DD 35 in culture media with 1% FBS. The 3D retinas expressed rhodopsin, S- and M-opsins, but most cone photoreceptors expressed either S- or M-opsins. CONCLUSION: By modifying culture conditions in the SFEBq protocol, we obtained rod-dominated 3D retinas and S- and M-opsin expressing 3D retinas.
(MeSH Terms)

Contact
Akishi Onishi , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Retinal Regeneration
Contributors
Kaori Ueda, Akishi Onishi, Shin-ichiro Ito, Makoto Nakamura, Masayo Takahashi


Dataset List of 124-Ueda-miPSRetina

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 4867
Datast ID DD17_Rod_CRX_EtOH
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4868
Datast ID DD17_Rod_CRX_RA
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4869
Datast ID DD17_Rod_CRX_T3
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4870
Datast ID DD17_Sop_Trb2_EtOH
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4871
Datast ID DD17_Sop_Trb2_RA
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4872
Datast ID DD17_Sop_Trb2_T3
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4873
Datast ID DD20_Rod_CRX_EtOH
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4874
Datast ID DD20_Rod_CRX_RA
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4875
Datast ID DD20_Rod_CRX_T3
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4876
Datast ID DD20_Sop_RXRg_EtOH
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4877
Datast ID DD20_Sop_RXRg_RA
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4878
Datast ID DD20_Sop_RXRg_T3
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4879
Datast ID DD20_Sop_Trb2_EtOH
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4880
Datast ID DD20_Sop_Trb2_RA
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4881
Datast ID DD20_Sop_Trb2_T3
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4882
Datast ID DD25_Rod_CRX_E
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4883
Datast ID DD25_Rod_CRX_R
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4884
Datast ID DD25_Rod_CRX_T
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4885
Datast ID DD25_Rod_Sop_E
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4886
Datast ID DD25_Sop_RXRg_R
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4887
Datast ID DD25_Sop_RXRg_T
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4888
Datast ID DD25_Sop_Trb2_E
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4889
Datast ID DD25_Sop_Trb2_EtOH
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4890
Datast ID DD25_Sop_Trb2_R
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4891
Datast ID DD25_Sop_Trb2_T
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4892
Datast ID DD30_Rod_CRX_EtOH
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4893
Datast ID DD30_Rod_CRX_RA
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4894
Datast ID DD30_Rod_CRX_T3
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4895
Datast ID DD30_Sop_Trb2_EtOH
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4896
Datast ID DD30_Sop_Trb2_RA
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 4897
Datast ID DD30_Sop_Trb2_T3
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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