SSBD:database

Project ID

487-Tasaka-SMTmodulation

Project SID

487

Title

Submedius Thalamic Inputs Shape the Presynaptic Architecture of Layer 5 Orbitofrontal Cortex in Mice

Description

The orbitofrontal cortex (OFC) is central to cognitive and social functions, yet its presynaptic partners remain incompletely defined. In female mice, the OFC modulates infant-directed caregiving behaviors in an experience-dependent manner. Here, we identify the submedius thalamus (SMT) as a major presynaptic partner of the OFC. Trans-synaptic tracing revealed intensive inputs from both the SMT and mediodorsal thalamus (MD) to OFC layer 5 excitatory neurons. We generated a Tnnt1-Cre mouse line that enables selective targeting of these higher-order thalamic nuclei. Axonal tracing demonstrated complementary projection patterns of the SMT and MD across prefrontal regions. Microendoscopic Ca2+ imaging demonstrated pup retrieval-related activity in both SMT and MD, with SMT exhibiting learning-related plasticity. Projection-specific chemogenetic silencing demonstrated that SMT modulates OFC activity during pup retrieval, although its behavioral relevance appeared limited. Collectively, our study provides a presynaptic map of OFC layer 5 neurons and a proof of concept for selective manipulation of the SMT as a previously understudied thalamic input to the OFC.

Submission Date

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Opened Date

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Release Date

2026-07-09

Update Date

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Kind

License

Project URL

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Project DOI

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Metadata Version

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Template Version

3.0.3

Funding Information

This work was supported by the JST PRESTO program (JPMJPR21S7), JSPS KAKENHI (20K15941 and 25K18590), RIKEN incentive and diversity promotion grants to G.T., and by a Takeda Science Foundation Research Grant, JSPS KAKENHI (21H02587 and 25K02368) to K.M.

Total

38 files / 268.7 GB

Image datasets

zipped 19 files / 97.3 GB, raw image 19 files / 171.4 GB, total 38 files / 268.7 GB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

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Cell

Cell line

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MeSH

Anatomical Entity

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UBERON

Biological Process

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Cellular Component

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Molecular Function

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Medical Subject Headings

Method involved in biological imaging