469-Ishii-pulseSTED
Author reviewedSuper resolution image of dendritic spine taken by new STED microscopy
Project ID
469-Ishii-pulseSTED
Project SID
469
Title
Super resolution image of dendritic spine taken by new STED microscopy
Description
Super-resolution in two-photon excitation (2PE) microscopy offers new approaches for visualizing the deep inside the brain functions at the nanoscale. In this study, the authors developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time-gated fluorescence detection, namely, all-pulsed 2PE-gSTED microscopy. The implementation of time-gating is critical to excluding undesirable signals derived from brain tissues. Even in a case using subnanosecond pulses for STED, the impact of time-gating was not negligible; the spatial resolution in the image of the brain tissue was improved by approximately 1.4 times compared with non time-gated image. This finding demonstrates that time-gating is more useful than previously thought for improving spatial resolution in brain tissue imaging. This microscopy will facilitate deeper super-resolution observation of the fine structure of neuronal dendritic spines and the intracellular dynamics in brain tissue.
Submission Date
2025-09-04
Opened Date
-
Release Date
2026-07-08
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
Template Version_3.0
Funding Information
This study was supported by: • the AMED
Brain/MINDS (JP19dm0207078) • the JST CREST
(JPMJCR20E4) • the MEXT/JSPS KAKENHI
(JP15H05953 “Resonance Bio,”), (JP16H06280
“Advanced Bioimaging Support,”), (JP18K14659),
(JP20H00523), (JP20H05669), (JP21K19346),
(JP22H02756) • the Research Foundation for
Opto-Science and Technology • the ExCELLS
“Encouragement Research for Young Scientists”.
Total
20 files / 113.7 MB
Image datasets
zipped 10 files / 35.2 MB, raw image 10 files / 78.6 MB, total 20 files / 113.7 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
B6.Cg-Tg(Thy1-YFP)HJrs/J
(
https://www.jax.org/strain/003782
)
Cell
neuron
(
CL:0000540
)
Cell line
-
MeSH
Ontology
Anatomical Entity
-
UBERON
brain
(
UBERON:0000955
)
Biological Process
-
Cellular Component
dendritic spine
(
GO:0043197
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
two-photon laser scanning microscopy
(
FBbi:00000254
)
stimulated emission depletion (STED)
(
FBbi:00000334
)
Paper DOI / Paper URL
People
Contact
Tomomi Nemoto
National Institutes of Natural
Sciences
Exploratory Research Center on Life and Living Systems
-
-
Imaging dataset contributor
Hirokazu Ishii (National Institutes of Natural
Sciences)
Kohei Otomo (National Institutes of Natural
Sciences)
Ching-Pu Chang (National Institutes of Natural
Sciences)
Tomomi Nemoto (National Institutes of Natural
Sciences)
Miwako Yamasaki (Hokkaido University)
Masahiko Watanabe (Hokkaido University)
Hiroyuki Yokoyama (Tohoku University)
Quantitative dataset contributor
-
Image datasets
10
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
2PE image of the neuronal dendrites in 4 micrometer depth of fixed Thy1-YFP-H mouse brain
2PE-STED image of the neuronal dendrites in 4 micrometer depth of fixed Thy1-YFP-H mouse brain
2PE-gSTED image of the neuronal dendrites in 4 micrometer depth of fixed Thy1-YFP-H mouse brain
2PE image of the neuronal dendrites in 36 micrometer depth of fixed Thy1-YFP-H mouse brain.
2PE-STED image of the neuronal dendrites in 36 micrometer depth of fixed Thy1-YFP-H mouse brain
2PE-gSTED image of the neuronal dendrites in 36 micrometer depth of fixed Thy1-YFP-H mouse brain
Z-series images of immunostained Thy1-YFP-H mouse brain
2PE image of the neuronal dendrites in immunostained Thy1-YFP-H mouse brain
2PE-STED image of the neuronal dendrites in immunostained Thy1-YFP-H mouse brain
2PE-gSTED image of the neuronal dendrites in immunostained Thy1-YFP-H mouse brain