456-Ezaki-FESEMCellProlife
Author reviewedImages of proliferating cells in Arabidopsis leaf
Project ID
456-Ezaki-FESEMCellProlife
Project SID
456
Title
Images of proliferating cells in Arabidopsis leaf
Description
During leaf development, the timing of transition from cell proliferation to expansion is an important factor in determining the final organ size. However, the regulatory system involved in this transition remains less understood. To get an insight into this system, we investigated the compensation phenomenon, in which the cell number decreases while the cell size increases in organs with determinate growth. Compensation is observed in several plant species suggesting coordination between cell proliferation and expansion. In this study, we examined an Arabidopsis mutant of ANGUSTIFOLIA 3 (AN3)/GRF-INTERACTING FACTOR 1, a positive regulator of cell proliferation, which exhibits the compensation. Though the AN3 role has been extensively investigated, the mechanism underlying excess cell expansion in the an3 mutant remains unknown. Focusing on the early stage of leaf development, we performed kinematic, cytological, biochemical, and transcriptome analyses, and found that the cell size had already increased during the proliferation phase, with active cell proliferation in the an3 mutant. Moreover, at this stage, chloroplasts, vacuoles, and xylem cells developed earlier than in the wild-type cells. Transcriptome data showed that photosynthetic activity and secondary cell wall biosynthesis were activated in an3 proliferating cells. These results indicated that precocious cell differentiation occurs in an3 cells. Therefore, we suggest a novel AN3 role in the suppression of cell expansion/differentiation during the cell proliferation phase.
Submission Date
-
Opened Date
-
Release Date
2026-07-07
Update Date
-
Kind
Image datasets datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
3.0.2
Funding Information
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by a Grand-in-Aid for Scientific Research on Innovation Areas (HT, JP25113002 and JP19H05672) from MEXT.
Total
122 files / 13.1 GB
Image datasets
zipped 61 files / 5.5 GB, raw image 61 files / 7.5 GB, total 122 files / 13.1 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Arabidopsis thaliana
Strain
Col-0
(
https://abrc.osu.edu/stocks/1
)
an3-4
Cell
vacuole
plasma membrane
(
GO:0005886
)
adaxial subepidermal cells
Cell line
plasma membrane
(
GO:0005886
)
MeSH
Ontology
Anatomical Entity
-
UBERON
primordium
(
UBERON:0001048
)
Biological Process
cell population proliferation
(
GO:0008283
)
cell differentiation
(
GO:0030154
)
cell division
(
GO:0051301
)
Cellular Component
vacuole
(
GO:0005773
)
plasma membrane
(
GO:0005886
)
chloroplast
(
GO:0009507
)
thylakoid membrane
(
GO:0042651
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
Paper DOI / Paper URL
People
Contact
Kazune Ezaki
The University of Tokyo
Department of Biological Sciences, Graduate School of Science
Laboratory of Eco-Devo Plant Biology
7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Hirokazu Tsukaya
The University of Tokyo
Department of Biological Sciences, Graduate School of Science
Laboratory of Eco-Devo Plant Biology
7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Imaging dataset contributor
Kazune Ezaki (The University of Tokyo)
Quantitative dataset contributor
-
Image datasets
61
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample1 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample3 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample4 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample5 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample1 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample2 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample4 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample5 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2_x6000
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2_x6000
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2_x8000
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample1
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample2
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample1 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample2 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample3 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample4 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample5 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample6 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample7 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of WT-Arabidopsis thaliana_sample8 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample1 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample2 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample4 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample5 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample6 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)