451-Hitora-Neopetromin
Author reviewedThe shape of vacuole and F-actin in tobacco BY-2 cells treated with neopetromin, a newly found peptide
Project ID
451-Hitora-Neopetromin
Project SID
451
Title
The shape of vacuole and F-actin in tobacco BY-2 cells treated with neopetromin, a newly found peptide
Description
HPLC-MS analysis revealed the presence of an
unreported peptide in the extract of the marine sponge Neopetrosia
sp. Its structure was determined as a tripeptide, named
neopetromin (1), composed of two tyrosine and one tryptophan
residues with a heteroaromatic C−N cross-link between side
chains. The absolute configuration of amino acids was determined
using Marfey’s method after ozonolysis and hydrolysis of 1.
Compound 1 promoted vacuole fragmentation in an actin-
independent manner in tobacco BY-2 cells.
Submission Date
2025-07-03
Opened Date
-
Release Date
2025-11-28
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This work was supported by JSPS KAKENHI Grant Number
26305005 (S.T.) and the Sumitomo Foundation Grant
Number 2330082 (T.H.).
Total
12 files / 720.7 MB
Image datasets
zipped 6 files / 101.4 MB, raw image 6 files / 619.2 MB, total 12 files / 720.7 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Nicotiana tabacum
(
NCBITaxon:4097
)
Strain
-
Cell
-
Cell line
TBY-2 cell
(
BTO:0001972
)
MeSH
Ontology
Anatomical Entity
-
UBERON
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Biological Process
negative regulation of actin filament polymerization
(
GO:0030837
)
Cellular Component
vacuole
(
GO:0005773
)
actin cytoskeleton
(
GO:0015629
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
Paper DOI / Paper URL
People
Contact
Sachiko Tsukamoto
Kumamoto
University
Department of Natural Medicines,
Graduate School of Pharmaceutical Sciences
-
-
Yuki Hitora
Kumamoto
University
Department of Natural Medicines,
Graduate School of Pharmaceutical Sciences
-
-
Imaging dataset contributor
-
Quantitative dataset contributor
-
Image datasets
6
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Z-series images of GFP-AtVam3p-labeled vacuolar membranes in tobacco BY-2 cells in the presence of latrunculin B
Z-series images of GFP-AtVam3p-labeled vacuolar membranes in tobacco BY-2 cells in the presence of neopetromin
Z-series images of GFP-AtVam3p-labeled vacuolar membranes in tobacco BY-2 cells in the absence of any inhivbitors
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of latrunculin B
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of neopetromin
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the absence of any inhivbitors