SSBD:database

Project ID

416-Otomo-descSPIM

Project SID

416

Title

Images of brain and xenograft obtained by descSPIM, the desktop- equipped SPIM

Description

Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop- equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low- expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three- dimensional manner. Academically open-sourced (https://github.com/dbsb- juntendo/descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.

Submission Date

2025-03-17

Opened Date

-

Release Date

2025-11-28

Update Date

-

Kind

License

Project URL

-

Project DOI

-

Metadata Version

-

Template Version

v3.0.1

Funding Information

This study was supported by the Japan Agency for Medical Research and Development (AMED) PRIME (to E.A.S., grant number JP20gm6210027); AMED Research on Development of New Drugs (to A.H. and E.A.S., grant number JP21ak0101181); AMED Brain/MINDS (to E.A.S. and T.Nemoto, grant number JP21wm0425003, and JP19dm0207078); AMED Project for Promotion of Cancer Research and Therapeutic Evolution (P-PROMOTE) (to E.A.S., grant number JP22ama221517); Japan Science and Technology Agency (JST) CREST (to K.Otomo and E.A.S., grant number JPMJCR20E4 and JPMJCR23B7); Japan Society for the Promotion of Science (JSPS) KAKENHI grant-in-aid for scientific research (B) (to K.Otomo and E.A.S., grant number JP22H02756 and JP22H02824); JSPS KAKENHI grant-in-aid for scientific research (S) (to T.Nemoto and K.Otomo, grant number JP20H05669); JSPS KAKENHI grant-in-aid for challenging research (exploratory) (to K.Otomo, grant number JP21K19346); JSPS KAKENHI grant-in-aid for transformative research areas - platforms for advanced technologies and research resources “Advanced Bioimaging Support” (to T.Nemoto and E.A.S., grant number JP22H04926); JSPS KAKENHI grant-in-aid for fund for the promotion of joint international research (Fostering Joint International Research(B)) (to K.Otomo, grant number JP22KK0100); JSPS KAKENHI for international leading research (to E.A.S., grant number JP23K20044); Operating Costs Subsidies for Private Universities (to E.A.S.); Grants-in-Aid from UTEC-UTokyo (to E.A.S.); the Takeda Science Foundation (to E.A.S.); Nakatani foundation for advancement of measuring technologies in biomedical engineering (to E.A.S.); The Mochida Memorial Foundation for Medical and Pharmaceutical Research (to E.A.S.), and The Uehara Memorial Foundation (to E.A.S.).

Total

125 files / 767.9 GB

Image datasets

zipped 20 files / 35.3 GB, raw image 50 files / 348.6 GB, total 70 files / 383.9 GB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

-

Cell

Cell line

MeSH

Anatomical Entity

-

UBERON

Biological Process

-

Cellular Component

Molecular Function

-

Medical Subject Headings

Method involved in biological imaging