SSBD:database

388-Okuno-CFinput

Author reviewed

Climbing fiber inputs to Purkinje cells in wild type mice and PTPdelta knockout mice

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Project ID

388-Okuno-CFinput

Project SID

388

Title

Climbing fiber inputs to Purkinje cells in wild type mice and PTPdelta knockout mice

Description

Functionally mature neural circuits are shaped during postnatal development by eliminating redundant synapses formed during the perinatal period. In the cerebellum of neonatal rodents, each Purkinje cell (PC) receives synaptic inputs from multiple (more than 4) climbing fibers (CFs). During the first 3 postnatal weeks, synaptic inputs from a single CF become markedly larger and those from the other CFs are eliminated in each PC, leading to mono-innervation of each PC by a strong CF in adulthood. While molecules involved in the strengthening and elimination of CF synapses during postnatal development are being elucidated, much less is known about the molecular mechanisms underlying CF synapse formation during the early postnatal period. The authors show experimental evidence that suggests that a synapse organizer, PTPdelta, is required for early postnatal CF synapse formation and the subsequent establishment of CF to PC synaptic wiring. They showed that PTPdelta was localized at CF-PC synapses from postnatal day 0 (P0) irrespective of the expression of Aldolase C (Aldoc), a major marker of PC that distinguishes the cerebellar compartments. They found that the extension of a single strong CF along PC dendrites (CF translocation) was impaired in global PTPdelta knockout (KO) mice from P12 to P29-31 predominantly in PCs that did not express Aldoc [Aldoc (–) PCs].

Submission Date

2024-12-02

Opened Date

Release Date

2025-11-26

Update Date

Kind

Image datasets

License

CC BY

Project URL

Project DOI

Metadata Version

Template Version

Funding Information

Biosample

Organism

Mus musculus ( NCBITaxon:10090 )

Strain

C57BL/6N, C57BL/6J, 129/SvJ ( https://www.jax.org/strain/000664 )

Cell

neuron, Purkinje cell ( CL:0000121 )

Cell line

MeSH

Neurons, Purkinje Cells ( D018613 )

Brain

Growth and Development

Synapses, Cell Body

Ontology

Anatomical Entity

UBERON

brain ( UBERON:0000955 )

Biological Process

nervous system development ( GO:0007399 )

Cellular Component

synapse, cell body ( GO:0044297 )

Molecular Function

Medical Subject Headings

Neurons, Purkinje Cells ( D018613 )

Brain

Growth and Development

Synapses, Cell Body

Imaging Method

Method involved in biological imaging

confocal microscopy ( FBbi:00000251 )

Paper DOI / Paper URL

PMID: 37426069 , doi: 10.3389/fnmol.2023.1206245

People

Contact

Takaki Watanabe

The University of Tokyo

Department of Neurophysiology, Graduate School of Medicine

Masanobu Kano

The University of Tokyo

Department of Neurophysiology, Graduate School of Medicine

Imaging dataset contributor

Yuto Okuno (The University of Tokyo)

Quantitative dataset contributor

Dataset List

Thumbnail

Dataset

Kind

Quantitative

Title

Fig1M_KO1_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1M_KO1_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1M_KO1_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1M_KO1_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1M_KO1_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO1_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO1_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO1_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO2_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1M_KO2_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1M_KO2_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1M_KO2_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1M_KO2_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO2_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO2_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO2_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO3_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1M_KO3_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1M_KO3_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1M_KO3_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1M_KO3_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO3_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO3_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_KO3_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1M_WT1_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1M_WT1_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1M_WT1_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1M_WT1_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1M_WT1_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT1_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT1_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT1_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT2_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1M_WT2_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1M_WT2_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1M_WT2_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1M_WT2_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT2_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT2_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT2_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT3_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1M_WT3_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1M_WT3_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1M_WT3_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1M_WT3_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT3_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT3_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1M_WT3_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1O_KO1_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1O_KO1_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1O_KO1_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1O_KO1_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1O_KO1_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1O_KO1_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1O_KO1_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1O_KO1_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1O_KO2_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1O_KO2_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in PTPdelta-KO mouse

Fig1O_KO2_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1O_KO2_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in PTPdelta-KO mouse

Fig1O_KO2_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1O_KO2_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1O_KO2_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1O_KO2_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in PTPdelta-KO mouse

Fig1O_WT1_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1O_WT1_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1O_WT1_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1O_WT1_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1O_WT1_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1O_WT1_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1O_WT1_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1O_WT1_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1O_WT2_Lobule1_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1O_WT2_Lobule1_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule1 in wild type mouse

Fig1O_WT2_Lobule3_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1O_WT2_Lobule3_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse

Fig1O_WT2_Lobule9_1

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1O_WT2_Lobule9_2

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1O_WT2_Lobule9_3

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig1O_WT2_Lobule9_4

Image data

Z-series images of synaptic inputs to the cerebellar lobule9 in wild type mouse

Fig2A_P0_Aldoc-Nega

Image data

Z-series images of PTPdelta localization in Aldoc-negative area in the P0 mouse cerebellum

Fig2A_P18_Aldoc-Nega

Image data

Z-series images of PTPdelta localization in Aldoc-negative area in the P18 mouse cerebellum

Fig2A_P18_Aldoc-Posi

Image data

Z-series images of PTPdelta localization in Aldoc-positive area in the P18 mouse cerebellum

Fig2A_P6_Aldoc-Nega

Image data

Z-series images of PTPdelta localization in Aldoc-negative area in the P6 mouse cerebellum

Fig2A_P6_Aldoc-Posi

Image data

Z-series images of PTPdelta localization in Aldoc-positive area in the P6 mouse cerebellum