Description
Functionally mature neural circuits are shaped during postnatal development
by eliminating redundant synapses formed during the perinatal period. In the
cerebellum of neonatal rodents, each Purkinje cell (PC) receives synaptic inputs
from multiple (more than 4) climbing fibers (CFs). During the first 3 postnatal
weeks, synaptic inputs from a single CF become markedly larger and those from
the other CFs are eliminated in each PC, leading to mono-innervation of each PC
by a strong CF in adulthood. While molecules involved in the strengthening and
elimination of CF synapses during postnatal development are being elucidated,
much less is known about the molecular mechanisms underlying CF synapse
formation during the early postnatal period. The authors show experimental evidence
that suggests that a synapse organizer, PTPdelta, is required for early postnatal CF
synapse formation and the subsequent establishment of CF to PC synaptic wiring.
They showed that PTPdelta was localized at CF-PC synapses from postnatal day 0
(P0) irrespective of the expression of Aldolase C (Aldoc), a major marker of PC
that distinguishes the cerebellar compartments. They found that the extension of
a single strong CF along PC dendrites (CF translocation) was impaired in global
PTPdelta knockout (KO) mice from P12 to P29-31 predominantly in PCs that did
not express Aldoc [Aldoc (–) PCs].
Contacts
Takaki
Watanabe
(Contact)
, Department of Neurophysiology, Graduate School of Medicine
, The University of Tokyo
Masanobu
Kano
(Contact)
, Department of Neurophysiology, Graduate School of Medicine
, The University of Tokyo
Yuto
Okuno
(Imaging Contributor)
, Department of Neurophysiology, Graduate School of Medicine
, The University of Tokyo
UBERON
Molecular biological information
Protein names
Fructose-bisphosphate aldolase C (
P05063)
Vesicular glutamate transporter 2 (
Q8BLE7)
Receptor-type tyrosine-protein phosphatase delta (
Q64487)
Carbonic anhydrase-related protein (
P28651)
Reagent or Compound
Concentration
Fold Dilution
Dimensions
1024x1024x5x4x1,
640x640x20x3x1,
1024x1024x10x4x1,
1024x1024x20x3x1,
X scales
0.047 micrometer,
0.26 micrometer,
0.12 micrometer,
0.31 micrometer,
0.18 micrometer,
0.06 micrometer,
0.09 micrometer,
Y scales
0.047 micrometer,
0.26 micrometer,
0.12 micrometer,
0.31 micrometer,
0.18 micrometer,
0.06 micrometer,
0.09 micrometer,
Z scales
0.48 micrometer,
0.5 micrometer,
0.61 micrometer,
Channels
4 channel,
3 channel,
Biological Imaging Methods Ontology
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods
Body
Olympus-Evident FV1200
Objective
Olympus-Evident UPLSAPO40X
Body
Olympus-Evident FV1200
Objective
Olympus-Evident UPLSAPO60XO
Body
Olympus-Evident FV1200
Objective
Olympus-Evident UPLSAPO20X
Dataset name
Organism
Gene / Protein
Dimensions
File size
SSBD:OMERO
Download
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
640x640x20x3x1
47MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
Ptprd
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
1024x1024x20x3x1
120MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
/ Receptor-type tyrosine-protein phosphatase delta (
Q64487)
1024x1024x5x4x1
40MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
/ Receptor-type tyrosine-protein phosphatase delta (
Q64487)
1024x1024x10x4x1
80MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
/ Receptor-type tyrosine-protein phosphatase delta (
Q64487)
1024x1024x10x4x1
80MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
/ Receptor-type tyrosine-protein phosphatase delta (
Q64487)
1024x1024x10x4x1
80MB
/ Carbonic anhydrase-related protein (
P28651)
/ Fructose-bisphosphate aldolase C (
P05063)
/ Vesicular glutamate transporter 2 (
Q8BLE7)
/ Receptor-type tyrosine-protein phosphatase delta (
Q64487)
1024x1024x10x4x1
80MB