383-Hiromoto-PoralizeDiv
Author reviewedTime-lapse images of intracellular structure in elongating Arabidopsis zygotes
Project ID
383-Hiromoto-PoralizeDiv
Project SID
383
Title
Time-lapse images of intracellular structure in elongating Arabidopsis zygotes
Description
A comprehensive and quantitative evaluation of multiple intracellular structures or proteins is a promising approach to provide a deeper understanding of and new insights into cellular polarity. In this study, the authors developed an image analysis pipeline to obtain intensity profiles of fluorescent probes along the apical–basal axis in elongating Arabidopsis thaliana zygotes based on two-photon live-cell imaging data. This technique showed the intracellular distribution of actin filaments, mitochondria, microtubules, and vacuolar membranes along the apical–basal axis in elongating zygotes from the onset of cell elongation to just before asymmetric cell division. Hierarchical cluster analysis of the quantitative data on intracellular distribution revealed that the zygote may be compartmentalized into two parts, with a boundary located 43.6% from the cell tip, immediately after fertilization. To explore the biological significance of this compartmentalization, they examined the positions of the asymmetric cell divisions from the dataset used in this distribution analysis. They found that the cell division plane was reproducibly inserted 20.5% from the cell tip. This position corresponded well with the midpoint of the compartmentalized apical region, suggesting a potential relationship between the zygote compartmentalization, which begins with cell elongation, and the position of the asymmetric cell division.
Submission Date
2024-08-28
Opened Date
-
Release Date
2025-11-26
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This work was supported by the Japan Advanced Plant Science Network, the Japan Society for the Promotion of Science [a Grant-in-Aid for Research Activity Start-up (JP21K20650 to H.M.), a Grant-in-Aid for Early-Career Scientists (JP22K15135 to H.M., and JP23K14204 to Y.K.), a Grant-in-Aid for Scientific Research on Innovative Areas (JP19H05670 and JP19H05676 to M.U.; JP16H06280 (Advanced Bio- imaging Support)), a Grant-in-Aid for Scientific Research (B) (JP23H02494 to M.U.)], and the Japan Science and Technology Agency [CREST (JPMJCR2121 to T. H. and M.U.)].
Total
156 files / 10.9 GB
Image datasets
zipped 24 files / 2.5 GB, raw image 132 files / 8.4 GB, total 156 files / 10.9 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Arabidopsis thaliana
(
NCBITaxon:3702
)
Strain
Col-0
(
https://abrc.osu.edu/stocks/8100
)
Cell
zygote
(
CL:0010017
)
Cell line
-
MeSH
Zygote
(
D036641, D059008
)
Zygote
Asymmetric Cell Division,
Cell Polarity
Actins
Zygote
(
D018613, D059009
)
Zygote
(
D018613, D059010
)
Zygote
(
D018613, D059011
)
Zygote
(
D018613, D059012
)
Zygote
(
D018613, D059013
)
Zygote
(
D018613, D059020
)
Microtubules
Zygote
(
D018613, D059021
)
Zygote
(
D018613, D059022
)
Zygote
(
D018613, D059023
)
Zygote
(
D018613, D059024
)
Zygote
(
D018613, D059025
)
Zygote
(
D018613, D059014
)
Mitochondria
Zygote
(
D018613, D059015
)
Zygote
(
D018613, D059016
)
Zygote
(
D018613, D059017
)
Zygote
(
D018613, D059018
)
Zygote
(
D018613, D059019
)
Zygote
(
D018613, D059026
)
Vacuoles
Zygote
(
D018613, D059027
)
Zygote
(
D018613, D059028
)
Zygote
(
D018613, D059029
)
Zygote
(
D018613, D059030
)
Zygote
(
D018613, D059031
)
Ontology
Anatomical Entity
-
UBERON
zygote stage
(
UBERON:0000106
)
Biological Process
zygote asymmetric cell division,
cellular component organization
(
GO:0016043
)
Cellular Component
actin cytoskeleton
(
GO:0015629
)
microtubule cytoskeleton
(
GO:0015630
)
mitochondrion
(
GO:0005739
)
vacuole
(
GO:0005773
)
Molecular Function
-
Medical Subject Headings
Zygote
(
D036641, D059008
)
Zygote
Asymmetric Cell Division,
Cell Polarity
Actins
Zygote
(
D018613, D059009
)
Zygote
(
D018613, D059010
)
Zygote
(
D018613, D059011
)
Zygote
(
D018613, D059012
)
Zygote
(
D018613, D059013
)
Zygote
(
D018613, D059020
)
Microtubules
Zygote
(
D018613, D059021
)
Zygote
(
D018613, D059022
)
Zygote
(
D018613, D059023
)
Zygote
(
D018613, D059024
)
Zygote
(
D018613, D059025
)
Zygote
(
D018613, D059014
)
Mitochondria
Zygote
(
D018613, D059015
)
Zygote
(
D018613, D059016
)
Zygote
(
D018613, D059017
)
Zygote
(
D018613, D059018
)
Zygote
(
D018613, D059019
)
Zygote
(
D018613, D059026
)
Vacuoles
Zygote
(
D018613, D059027
)
Zygote
(
D018613, D059028
)
Zygote
(
D018613, D059029
)
Zygote
(
D018613, D059030
)
Zygote
(
D018613, D059031
)
Imaging Method
Method involved in biological imaging
two-photon laser scanning microscopy
(
FBbi:00000254
)
time lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Takumi Higaki
Kumamoto University
Graduate School of Science and Technology
-
2-39-1 Kurokami, Chuo-Ku, Kumamoto 860-8555, Japan
Imaging dataset contributor
Yusuke Kimata (Tohoku University)
Hikari Matsumoto (Tohoku University)
Sakumi Nakagawa (Tohoku University)
Minako Ueda (Tohoku University)
Quantitative dataset contributor
-
Image datasets
24
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse and z-series images of actin filament in elongating Arabidopsis zygotes
Time-lapse and z-series images of actin filament in elongating Arabidopsis zygotes
Time-lapse and z-series images of actin filament in elongating Arabidopsis zygotes
Time-normalized images of actin filament in elongating Arabidopsis zygotes
Time-normalized images of actin filament in elongating Arabidopsis zygotes
Time-normalized images of actin filament in elongating Arabidopsis zygotes
Time-lapse and z-series images of microtubule in elongating Arabidopsis zygotes
Time-lapse and z-series images of microtubule in elongating Arabidopsis zygotes
Time-lapse and z-series images of microtubule in elongating Arabidopsis zygotes
Time-normalized images of microtubule in elongating Arabidopsis zygotes
Time-normalized images of microtubule in elongating Arabidopsis zygotes
Time-normalized images of microtubule in elongating Arabidopsis zygotes
Time-lapse and z-series images of mitochondria in elongating Arabidopsis zygotes
Time-lapse and z-series images of mitochondria in elongating Arabidopsis zygotes
Time-lapse and z-series images of mitochondria in elongating Arabidopsis zygotes
Time-normalized images of mitochondria in elongating Arabidopsis zygotes
Time-normalized images of mitochondria in elongating Arabidopsis zygotes
Time-normalized images of mitochondria in elongating Arabidopsis zygotes
Time-lapse and z-series images of vacuolar membrane in elongating Arabidopsis zygotes
Time-lapse and z-series images of vacuolar membrane in elongating Arabidopsis zygotes
Time-lapse and z-series images of vacuolar membrane in elongating Arabidopsis zygotes
Time-normalized images of vacuolar membrane in elongating Arabidopsis zygotes
Time-normalized images of vacuolar membrane in elongating Arabidopsis zygotes
Time-normalized images of vacuolar membrane in elongating Arabidopsis zygotes