SSBD:database v3

373-Tomioka-CaImage

OMERO gallery,
OMERO index

Project

Title
Calcium imaging of ASEL gustatory neuron of C. elagans in response to glucose after conditioning
Description
The nematode Caenorhabditis elegans memorizes various external chemicals, such as ions and odorants, during feeding. Here the authors find that C. elegans is attracted to the monosaccharides glucose and fructose after exposure to these monosaccharides in the presence of food; however, it avoids them without conditioning. The attraction to glucose requires a gustatory neuron called ASEL. ASEL activity increases when glucose concentration decreases.
License
CC BY
Released at
Oct. 20, 2025, midnight
Updated at
Funding
M.T. was supported by Grants-in-Aid for Scientific Research (C) (18K06055) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT). Y.I. was supported by Grants-in-Aid for Scientific Research (A) (22H00416), for Scientific Research (S) (17H06113), for Scientific Research on Innovative Area (25115010) and for Scientific Research on Innovative Area (20115001) from MEXT.
# of image datasets
39

Papers

Papers
Paper information: Tomioka M, Umemura Y, Ueoka Y, Chin R, Katae K, Uchiyama C, Ike Y, Iino Y. Antagonistic regulation of salt and sugar chemotaxis plasticity by a single chemosensory neuron in Caenorhabditis elegans. PLoS Genet. 2023 Sep 5;19(9):e1010637. PLoS Genet. 2023 Sep 5;19(9):e1010637.,
Pubmed ID: 37669262,
PMCID: PMC10503759,
DOI: https://doi.org/10.1371/journal.pgen.1010637,
URL: https://pubmed.ncbi.nlm.nih.gov/37669262/

Contacts

Contacts
Masahiro Tomioka (Contact) , Division of Industrial Toxicology , National Institude of Occupational Safety and Health, Japan
Yuichi Iino (Contact) , Department of Biological Sciences, Graduate School of Science , The University of Tokyo
Risshun Chin (Imaging Contributor)

Biosamples

Organisms
Caenorhabditis elegans (NCBI:txid6239)
Strains
JN2103
Cells
neuron
Cell lines

Ontologies

UBERON
Anatomical entities
GO: Biological processes
response to chemical (GO:0042221) (GO:0042221)
GO: Cellular components
GO: Molecular functions

Genetics

Gene names
Protein names
Genetic methods
Protein tags
Probes
Oligo Primer
Genotype

Treatments

Reagent or Compound
Concentration
Fold Dilution
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)
glucose (CHEBI:17234)
0 or 15 millimolar (UO_0000063)

Imaging Methods

Dimensions
512x512x1x2x241,
X scales
0.4 micrometer,
Y scales
0.4 micrometer,
Z scales
NA,
T scales
0.5 seconds,
Channels
2 channel,
Microscopy types
fluorescence microscopy (FBbi:00000246), time lapse microscopy (FBbi:00000249)
Detection methods
Visualization methods
Illumination methods
Sources of contrast
Contrast enhancing methods
Resolution enhancing methods
Imaged parameters
Sample preparation methods

Instruments

Body
Leica DMI6000 B
Model
MAG BIOSYSTEMS DV2™ Dual-Channel, Simultaneous-Imaging System
Light source
Leica EL6000
Detector
Hamamatsu ImagEM EM-CCD
Objective
Leica HCX PL APO 63x/1,40
Filter set
Dichroic

Datasets

Dataset name
Organism
Gene / Protein
Dimensions
File size
SSBD:OMERO
Download
Fig2C_Ca_naiveWT_1
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_2
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_3
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_4
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_5
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_6
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_7
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_8
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_9
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_10
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_11
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_12
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_13
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_naiveWT_14
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_1
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_2
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_3
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_4
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_5
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_6
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_7
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_8
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_9
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_10
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_11
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_12
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionWT_13
Caenorhabditis elegans (NCBI:txid6239)
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_1
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_2
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_3
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_4
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_5
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_6
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_7
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_8
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_9
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_10
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_11
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip
Fig2C_Ca_conditionUNC13_12
Caenorhabditis elegans (NCBI:txid6239)
unc-13
512x512x1x2x241
128MB
Gallery, Index
zip