SSBD:database v3

Project

Project

373-Tomioka-CaImage

Title

Calcium imaging of ASEL gustatory neuron of C. elagans in response to glucose after conditioning

Description

The nematode Caenorhabditis elegans memorizes various external chemicals, such as ions and odorants, during feeding. Here the authors find that C. elegans is attracted to the monosaccharides glucose and fructose after exposure to these monosaccharides in the presence of food; however, it avoids them without conditioning. The attraction to glucose requires a gustatory neuron called ASEL. ASEL activity increases when glucose concentration decreases.

License

CC BY

Funding

M.T. was supported by Grants-in-Aid for Scientific Research (C) (18K06055) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT). Y.I. was supported by Grants-in-Aid for Scientific Research (A) (22H00416), for Scientific Research (S) (17H06113), for Scientific Research on Innovative Area (25115010) and for Scientific Research on Innovative Area (20115001) from MEXT.

Dataset

Title

Time-lapse calcium imaging of ASEL in naive WT C-elegans

Description

Time-lapse calcium imaging of ASEL, a gustatory neuron, in naive wild type C-elegans. The autors used YC2.60 as a calcium indicator, and measured fluorescence intensity ratio of YFP to CFP. Left-half are CFP images, and Right half are YFP images. The glucose concentration was switched from 15 to 0 mM at 50 seconds and then returned to 15 mM at 100 seconds.

License

CC BY

Submitted at

None

Released at

None

Updated at

Biosamples

Description

A left-sided ASE gustatory neuron in wild type C. elegans.

Organisms

Caenorhabditis elegans (NCBI:txid6239)

Strains

JN2103

Cells

neuron ()

Cell lines

Intrinsic variables

Extrinsic variables

gcy-7p::YC2.60 and lin-44p::mCherry were introduced as extrachromosomal arrays, glucose application

Ontologies

UBERON

Anatomical entities

MeSH: Neurons

Gene ontology: Biological processes

response to chemical (GO:0042221) (GO:0042221)

Gene ontology: Cellular components

Gene ontology: Molecular functions

Genetics

Gene names

Protein names

Genetic methods

Microinjection for YC2.60 introduction

Protein tags

Probes

YC2.60

Oligo Primer

Genotype

Treatments

Reagent or Compound

Concentrations

0 or 15 millimolar (UO_0000063)

FoldDilution

Imaging Methods

Dimensions

512x512x1x2x241

X scales

0.4 micrometer

Y scales

0.4 micrometer

Z scales

T scales

0.5 seconds

Channels

2 channel

Microscopy types

fluorescence microscopy (FBbi:00000246), time lapse microscopy (FBbi:00000249)

Detection methods

electron multiplying CCD (EMCCD)

Visualization methods

ECFP, Venus

Illumination methods

electron multiplying CCD (EMCCD)

Sources of contrast

Contrast enhancing methods

Resolution enhancing methods

Image parameters

Sample preparation methods

living tissue

Instruments

Body

Leica DMI6000 B

Model

Light source

Leica EL6000

Detector

Hamamatsu ImagEM EM-CCD

Objective

Leica HCX PL APO 63x/1,40

Filter set

Dichroic