Dataset Fig2C_Ca_conditionUNC13_8
Project
373-Tomioka-CaImage
Title
Calcium imaging of ASEL gustatory neuron of C. elagans in response to glucose after conditioning
Description
The nematode Caenorhabditis elegans memorizes various external chemicals, such as ions and odorants, during feeding. Here the authors find that C. elegans is attracted to the monosaccharides glucose and fructose after exposure to these monosaccharides in the presence of food; however, it avoids them without conditioning. The attraction to glucose requires a gustatory neuron called ASEL. ASEL activity increases when glucose concentration decreases.
Funding
M.T. was supported by Grants-in-Aid for Scientific Research (C) (18K06055) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT). Y.I. was supported by Grants-in-Aid for Scientific Research (A) (22H00416), for Scientific Research (S) (17H06113), for Scientific Research on Innovative Area (25115010) and for Scientific Research on Innovative Area (20115001) from MEXT.
Title
Time-lapse calcium imaging of ASEL in conditioned unc-13 mutant C-elegans
Description
Time-lapse calcium imaging of ASEL, a gustatory neuron, in conditioned unc-13 mutant C-elegans. The mutant worm was conditioned to be attracted to glucose beforehand. The autors used YC2.60 as a calcium indicator, and measured fluorescence intensity ratio of YFP to CFP. Left-half are CFP images, and Right half are YFP images. The glucose concentration was switched from 15 to 0 mM at 50 seconds and then returned to 15 mM at 100 seconds.
Description
A left-sided ASE gustatory neuron in unc-13 mutant C. elegans that received glucose conditioning to attract glucose.
Intrinsic variables
unc-13(e51) mutant, in which synaptic transmission is largely disrupted due to exocytosis defects
Extrinsic variables
gcy-7p::YC2.60 and lin-44p::mCherry were introduced as extrachromosomal arrays,
glucose application
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Genetic methods
EMS mutagenesis for unc-13(e51) introduction
Dimensions
512x512x1x2x241
Microscopy types
fluorescence microscopy (FBbi:00000246), time lapse microscopy (
FBbi:00000249)
Detection methods
electron multiplying CCD (EMCCD)
Visualization methods
ECFP, Venus
Illumination methods
electron multiplying CCD (EMCCD)
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods
living tissue
Light source
Leica EL6000
Detector
Hamamatsu ImagEM EM-CCD
Objective
Leica HCX PL APO 63x/1,40