Project
371-Liao-RamanSpectra
Title
Raman spectra data of cell spheroids on a multi-well plate treated with various concentrations of reagents
Description
Cell spheroids offer alternative in vitro cell models to
monolayer cultured cells because they express complexities similar to thoseof in vivo tissues, such as cellular responses to drugs and chemicals. Raman spectroscopy emerged as a powerful analytical tool for detecting chemical changes in living cells because it nondestructively provides vibrational information regarding a target. Although multiple iterations are required in drug screening to determine drugs to treat cell spheroids and assess the interspheroid heterogeneity, current Raman applications used in spheroidsanalysis allow the observation of only a few spheroids owing to the lowthroughput of Raman spectroscopy. In this study, the authors developed a multifocal Raman spectrophotometer that enables simultaneous analysis of multiple spheroids in separate wells of a regular 96-well plate. By utilizing 96 focal spots excitation and parallel signal collection, their system can improve the throughput by approximately 2 orders of magnitude compared to a conventional single-focus Raman microscope. The Raman spectra of HeLa cell spheroids treated with anticancer drugs and HepG2 cell spheroids treated with free fatty acids were measured simultaneously, and concentration-dependent cellular responses were observed in both studies. Using the multifocal Raman spectrophotometer, they rapidly observed chemical changes in spheroids, and thus, this system can facilitate the application of Raman spectroscopy in analyzing the cellular responses of spheroids.
Funding
This research was supported by Osaka University Innovation Bridge Grant and JST COI-NEXT under grant number JPMJPF2009.
Title
Raman spectrua data of HepG2 spheroids treated with various concentrations of FFA
Description
Raman spectra data of HepG2 spheroids treated with various concentrations of FFA (free fatty acid: 0 mM, 0.01 mM, 0.1 mM, 0.25 mM, 0.5 mM, 1.0 mM, 2.0 mM). Multiple spectra data is deposited for each concentration of FFA.
Description
HepG2 spheroids
Extrinsic variables
Treatment with several concentrations of free fatty acid
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Concentrations
0.01, 0.1, 0.25, 0.5, 1.0, 2.0
millimolar
(
UO_0000063)
Microscopy types
inelastic scattering of photons (Raman scattering) (
FBbi:00000589)
Detection methods
charge coupled device (CCD)
Visualization methods
Intensity of Raman Scattering
Illumination methods
charge coupled device (CCD)
Contrast enhancing methods
optical method
Resolution enhancing methods
Sample preparation methods
living spheroid cells
Detector
2D cooled CCD camera (PIXIS 400B eXcelon, Teledyne Princeton Instruments)
Filter set
Custom-made filter
Dichroic
Custom-made filter