SSBD:database

367-Tsuboi-DrosophilaWing

Author reviewed

The folding dynamics of Drosophila wing during pupal development

SSBD:downloads

Project ID

367-Tsuboi-DrosophilaWing

Project SID

367

Title

The folding dynamics of Drosophila wing during pupal development

Description

Biological systems are inherently noisy; however, they produce highly stereotyped tissue morphology. Drosophila pupal wings show a highly stereotypic folding through uniform expansion and subsequent buckling of wing epithelium within a surrounding cuticle sac. The folding pattern produced by buckling is generally stochastic; it is thus unclear how buckling leads to stereotypic tissue folding of the wings. The authors found that the extracellular matrix (ECM) protein, Dumpy, guides the position and direction of buckling-induced folds. Dumpy anchors the wing epithelium to the overlying cuticle at specific tissue positions. Tissue-wide alterations of Dumpy deposition and degradation yielded different buckling patterns. In summary, they propose that spatiotemporal ECM remodeling shapes stereotyped tissue folding through dynamic interactions between the epithelium and its external structures.

Submission Date

2024-11-08

Opened Date

Release Date

2025-11-28

Update Date

Kind

Image datasets and Quantitative datasets

License

CC BY

Project URL

Project DOI

Metadata Version

Template Version

Funding Information

This work was supported by JSPS KAKENHI 19J00764 (to A.T.), 21H05779 (to A.T.), 17H06386 (to K.F.), and 16H06280 “ABiS”; a grant from the NIPPON Genetics (to A.T.); The Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research (K- CONNEX) established by the program of Building of Consortia for the Development of Human Resources in Science and Technology, MEXT (to T.K.); and Japan Science and Technology Agency JPMJCR2121 (to K.F.).

Total

16770 files / 613.4 GB

Image datasets

zipped 23 files / 260.5 GB, raw image 16747 files / 353.0 GB, total 16770 files / 613.4 GB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Biosample

Organism

Drosophila ( NCBITaxon:7215 )

Strain

sd-Gal4/+; UAS-dpyRNAi/E-Cadher- in::GFP; UAS-mCD8::mCherry/UAS-Dcr2

sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-NpRNAi/+

sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-SbRNAi/+

sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP

Dpy::YFP, Ubi-GAP43::tdTomato/+; rho^{ve-1}, vn^{1}/+ ウエ

Dpy::YFP, Ubi-GAP43:: tdTomato/+; rho^{ve-1}, vn^{1}/rho^{ve-1}, vn^{1}

sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/+

sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/ UAS-NpRNAi

sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/+; UAS- SbRNAi/+

ap-GAL4/+; UAS-Dcr2/UAS-DsRed

y, w, hs-flp; E-Cadherin::GFP; Act > CD2 > GAL4; UAS-mCD8::mCherry

y, w, sqh::eGFP/Y; Tub-Gal80ts/+; UAS-Nslmb-vhhGFP4, UAS- mCD8::mCherry/ci-Gal4

y, w, sqh::eGFP/Y; Tub-Gal80ts/+; UAS-Nslmb-vhhGFP4, UAS- mCD8::mCherry/hh-Gal4

sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/Dpy::YFP

sd-Gal4/+; Dp::YFP, UAS-mCD8::mCherry/+

ap-GAL4/UAS-dpyRNAi; UAS-Dcr2/UAS-DsRed

Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; ci-Gal4/ UAS-3xFLAG.dCas9.VPR

Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; ci- Gal4/+

Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh-Gal4/ UAS-3xFLAG.dCas9.VPR

Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh- Gal4/+

Cell

Cell line

MeSH

( D018613 )

Pupa

growth & development

Cell Membrane

Extracellular Matrix

( D036641 )

Ontology

Anatomical Entity

pupa, wing ( FBbt:00004729 )

UBERON

pupa, wing ( UBERON:0000023 )

Biological Process

pupal development, pattern specification process ( GO:0007389 )

Cellular Component

plasma membrane ( GO:0005886 )

extracellular matrix ( GO:0031012 )

Molecular Function

Medical Subject Headings

( D018613 )

Pupa

growth & development

Cell Membrane

Extracellular Matrix

( D036641 )

Imaging Method

Method involved in biological imaging

confocal microscopy ( FBbi:00000251 )

time lapse microscopy ( FBbi:00000249 )

multi-photon microscopy ( FBbi:00000255 )

Paper DOI / Paper URL

PMID: 37656799 , doi: 10.1126/sciadv.adh2154

People

Contact

Alice Tsuboi

Kyoto University

Graduate School of Biostudies

Takefumi Kondo

Kyoto University

Graduate School of Biostudies

Imaging dataset contributor

Quantitative dataset contributor

Image datasets

Quantitative datasets

Dataset List

Thumbnail

Dataset

Organism

Kind / Links

FigS19_DpyKD

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings in Dpy knockdown Drosophila pupa expressing mCD8::mCherry and E-Cadherin::GFP

FigS19_NpKD

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings in Np knockdown pupa expressing mCD8::mCherry and E-Cadherin::GFP

FigS19_SbKD

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings in Sb knockdown Drosophila pupa expressing mCD8::mCherry and E-Cadherin::GFP

FigS19_ctrl

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings in control Drosophila pupa expressing mCD8::mCherry and E-Cadherin::GFP

MovieS10_ctrl

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in control Drosophila pupa

MovieS10_mutant

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in mutant Drosophila pupa lacking vein

MovieS11_DpyLocalize_1

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a control Drosophila wing expressing mCD8::mCherry and Dpy::YFP

MovieS11_DpyLocalize_2

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a control Drosophila wing expressing mCD8::mCherry and Dpy::YFP

MovieS12_NpKD

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a Drosophila wing expressing mCD8::mCherry and Dpy::YFP in Np knockdown Drosophila pupa

MovieS12_SbKD

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a Drosophila wing expressing mCD8::mCherry and Dpy::YFP in Sb knockdown Drosophila pupa

MovieS12_ctrl

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a Drosophila wing expressing mCD8::mCherry Dpy::YFP in control Drosophila pupa

MovieS1_FoldingWing

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a folding wing in Drosophila pupa

MovieS2_CellExpand

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a folding wing in Drosophila pupa expressing E-Cadherin-GFP

MovieS3_FoldingWing_sqhKO_Ant

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a folding wing in sqh-KO Drosophila pupa

MovieS4_FoldingWing_sqhKO_Post

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of a folding wing in sqh-KO Drosophila pupa

MovieS5_Dpy_1

Drosophila ( NCBITaxon:7215 )

Image data

Z-series images of Drosophila prefolded wing expressing mCD8::mCherry and Dpy::YFP

MovieS5_Dpy_2

Drosophila ( NCBITaxon:7215 )

Image data

Z-series images of Drosophila prefolded wing expressing mCD8::mCherry and Dpy::YFP

MovieS6_Dpy

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of Drosophila wing expressing mCD8::mCherry and Dpy::YFP

MovieS7_DpyKD_dorsal

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of Dpy-depleted Drosophila wing

MovieS8_DpyOE

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in Dpy overexpressing pupa

MovieS8_ctrl

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in control Drosophila pupa

MovieS9_DpyOE

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in Dpy overexpressing Drosophila pupa

MovieS9_ctrl

Drosophila ( NCBITaxon:7215 )

Image data

Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in control Drosophila pupa