367-Tsuboi-DrosophilaWing
Author reviewed
The folding dynamics of Drosophila wing during pupal development
Project ID
367-Tsuboi-DrosophilaWing
Title
The folding dynamics of Drosophila wing during pupal development
Description
Biological systems are inherently noisy; however, they produce highly stereotyped tissue morphology. Drosophila pupal wings show a highly stereotypic folding through uniform expansion and subsequent buckling of wing epithelium within a surrounding cuticle sac. The folding pattern produced by buckling is generally stochastic; it is thus unclear how buckling leads to stereotypic tissue folding of the wings. The authors found that the extracellular matrix (ECM) protein, Dumpy, guides the position and direction of buckling-induced folds. Dumpy anchors the wing epithelium to the overlying cuticle at specific tissue positions. Tissue-wide alterations of Dumpy deposition and degradation yielded different buckling patterns. In summary, they propose that spatiotemporal ECM remodeling shapes stereotyped tissue folding through dynamic interactions between the epithelium and its external structures.
Submission Date
2024-11-08
Kind
Image datasets and Quantitative datasets
Funding Information
This work was supported by JSPS KAKENHI 19J00764 (to A.T.), 21H05779 (to A.T.), 17H06386 (to K.F.), and 16H06280 “ABiS”; a grant from the NIPPON Genetics (to A.T.); The Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research (K- CONNEX) established by the program of Building of Consortia for the Development of Human Resources in Science and Technology, MEXT (to T.K.); and Japan Science and Technology Agency JPMJCR2121 (to K.F.).
Total
16770 files / 613.4 GB
Image datasets
zipped 23 files / 260.5 GB, raw image 16747 files / 353.0 GB, total 16770 files / 613.4 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
MeSH
Microscopy, Fluorescence, Multiphoton
(
D036641
)
Ontology
Medical Subject Headings
Microscopy, Fluorescence, Multiphoton
(
D036641
)
Imaging Method
Method involved in biological imaging
People
Contact
Alice Tsuboi
Kyoto University
Graduate School of Biostudies
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Takefumi Kondo
Kyoto University
Graduate School of Biostudies
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Imaging dataset contributor
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Quantitative dataset contributor
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Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Z-series and time-lapse images of wings in Dpy knockdown Drosophila pupa expressing mCD8::mCherry and E-Cadherin::GFP
Z-series and time-lapse images of wings in Np knockdown pupa expressing mCD8::mCherry and E-Cadherin::GFP
Z-series and time-lapse images of wings in Sb knockdown Drosophila pupa expressing mCD8::mCherry and E-Cadherin::GFP
Z-series and time-lapse images of wings in control Drosophila pupa expressing mCD8::mCherry and E-Cadherin::GFP
Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in control Drosophila pupa
Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in mutant Drosophila pupa lacking vein
Z-series and time-lapse images of a control Drosophila wing expressing mCD8::mCherry and Dpy::YFP
Z-series and time-lapse images of a control Drosophila wing expressing mCD8::mCherry and Dpy::YFP
Z-series and time-lapse images of a Drosophila wing expressing mCD8::mCherry and Dpy::YFP in Np knockdown Drosophila pupa
Z-series and time-lapse images of a Drosophila wing expressing mCD8::mCherry and Dpy::YFP in Sb knockdown Drosophila pupa
Z-series and time-lapse images of a Drosophila wing expressing mCD8::mCherry Dpy::YFP in control Drosophila pupa
Z-series and time-lapse images of a folding wing in Drosophila pupa
Z-series and time-lapse images of a folding wing in Drosophila pupa expressing E-Cadherin-GFP
Z-series and time-lapse images of a folding wing in sqh-KO Drosophila pupa
Z-series and time-lapse images of a folding wing in sqh-KO Drosophila pupa
Z-series images of Drosophila prefolded wing expressing mCD8::mCherry and Dpy::YFP
Z-series images of Drosophila prefolded wing expressing mCD8::mCherry and Dpy::YFP
Z-series and time-lapse images of Drosophila wing expressing mCD8::mCherry and Dpy::YFP
Z-series and time-lapse images of Dpy-depleted Drosophila wing
Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in Dpy overexpressing pupa
Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in control Drosophila pupa
Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in Dpy overexpressing Drosophila pupa
Z-series and time-lapse images of wings expressing mCD8::mCherry and Dpy::YFP in control Drosophila pupa