SSBD:database v3

367-Tsuboi-DrosophilaWing

Project

Title
The folding dynamics of Drosophila wing during pupal development
Description
Biological systems are inherently noisy; however, they produce highly stereotyped tissue morphology. Drosophila pupal wings show a highly stereotypic folding through uniform expansion and subsequent buckling of wing epithelium within a surrounding cuticle sac. The folding pattern produced by buckling is generally stochastic; it is thus unclear how buckling leads to stereotypic tissue folding of the wings. The authors found that the extracellular matrix (ECM) protein, Dumpy, guides the position and direction of buckling-induced folds. Dumpy anchors the wing epithelium to the overlying cuticle at specific tissue positions. Tissue-wide alterations of Dumpy deposition and degradation yielded different buckling patterns. In summary, they propose that spatiotemporal ECM remodeling shapes stereotyped tissue folding through dynamic interactions between the epithelium and its external structures.
License
CC BY
Released at
Oct. 20, 2025, midnight
Updated at
Funding
This work was supported by JSPS KAKENHI 19J00764 (to A.T.), 21H05779 (to A.T.), 17H06386 (to K.F.), and 16H06280 “ABiS”; a grant from the NIPPON Genetics (to A.T.); The Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research (K- CONNEX) established by the program of Building of Consortia for the Development of Human Resources in Science and Technology, MEXT (to T.K.); and Japan Science and Technology Agency JPMJCR2121 (to K.F.).
# of image datasets
23

Papers

Papers
Paper information: A. Tsuboi, K. Fujimoto, T. Kondo. Spatiotemporal remodeling of extracellular matrix orients epithelial sheet folding. Sci Adv. 2023 Sep;9(35):eadh2154,
Pubmed ID: 37656799,
PMCID: PMC10854429,
DOI: https://doi.org/10.1126/sciadv.adh2154,
URL: https://pubmed.ncbi.nlm.nih.gov/37656799/

Contacts

Contacts
Alice Tsuboi (Contact) , Graduate School of Biostudies , Kyoto University
Takefumi Kondo (Contact) , Graduate School of Biostudies , Kyoto University

Biosamples

Organisms
Drosophila (NCBI:txid7215)
Strains
y, w, sqh::eGFP/Y; Tub-Gal80ts/+; UAS-Nslmb-vhhGFP4, UAS- mCD8::mCherry/ci-Gal4
sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-SbRNAi/+
UAS-mCD8::mCherry
SbRNAi/+
sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP
Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; ci-Gal4/ UAS-3xFLAG.dCas9.VPR
sd-Gal4/+; UAS-mCD8::mCherry/E- Cadherin::GFP; UAS-NpRNAi/+
Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh- Gal4/+
ap-GAL4/UAS-dpyRNAi; UAS-Dcr2/UAS-DsRed
sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/+
y, w, sqh::eGFP/Y; Tub-Gal80ts/+; UAS-Nslmb-vhhGFP4, UAS- mCD8::mCherry/hh-Gal4
sd-Gal4/+; Dpy::YFP, UAS-mCD8::mCherry/Dpy::YFP
UAS-NpRNAi
sd-Gal4/+; Dp::YFP, UAS-mCD8::mCherry/+
Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; ci- Gal4/+
rho^{ve-1}, vn^{1}/rho^{ve-1}, vn^{1}
ap-GAL4/+; UAS-Dcr2/UAS-DsRed
Dpy::YFP, Ubi-GAP43::tdTomato/+; rho^{ve-1}, vn^{1}/+
sd-Gal4/+; UAS-dpyRNAi/E-Cadher- in::GFP; UAS-mCD8::mCherry/UAS-Dcr2
Dpy::YFP, UAS-mCD8::mCherry/dpy-gRNA; hh-Gal4/ UAS-3xFLAG.dCas9.VPR
Cells

Cell lines

Ontologies

UBERON
pupa (UBERON_0003143)
wing (UBERON_0000023)
Anatomical entities
GO: Biological processes
pupal development (GO:0035209)
GO: Cellular components
extracellular matrix (GO:0031012)
plasma membrane (GO:0005886)
GO: Molecular functions

Genetics

Gene names
Protein names
Myosin regulatory light chain sqh (P40423)
DE-cadherin (Q24298)
SP460 (Q9U3W7)
T-cell surface glycoprotein CD8 alpha chain (P01731)
Genetic methods
transgenic (EFO_0004020)
Protein tags
tdTomatoFP (FBbi:00000518)
mCherryFP (FBbi:00000525)
DsRed (FBbi:00000085)
Probes
Oligo Primer
Genotype

Treatments

Reagent or Compound
Concentration
Fold Dilution

Imaging Methods

Dimensions
512x512x100x2x57, 1024x1024x150x2x17, 1024x1024x150x2x17, 1024x1024x150x2x17, 1024x1024x150x2x19, 1024x1024x100x2x51, 1024x1024x100x2x47, 1024x1024x100x2x47, 1024x1024x100x2x47, 1024x1024x55x1x34, 512x512x100x2x54, 512x512x100x2x1, 1024x1024x85x2x23, 1024x1024x60x1x42, 1024x1024x100x2x54, 1024x1024x100x2x50, 1024x1024x150x2x19, 1024x1024x150x2x19, 1024x1024x100x2x44, 1024x1024x100x2x44, 1024x1024x100x2x49,
X scales
0.83 micrometer, 0.828 micrometer, 0.624 micrometer, 1.25 micrometer, 0.312 micrometer, 0.312 micrometer, 0.312 micrometer, 0.6919 micrometer, 0.692 micrometer,
Y scales
0.83 micrometer, 0.828 micrometer, 0.624 micrometer, 1.25 micrometer, 0.312 micrometer, 0.312 micrometer, 0.312 micrometer, 0.6919 micrometer, 0.692 micrometer,
Z scales
3 micrometer, 1 micrometer, ３ micrometer, 2 micrometer,
T scales
NA, 1 hour, 20 minutes,
Channels
2 channel, 1 channel,
Microscopy types
multi-photon microscopy (FBbi:00000255)
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection methods
Visualization methods
Illumination methods
Sources of contrast
Contrast enhancing methods
Resolution enhancing methods
Imaged parameters
Sample preparation methods

Instruments

Body
Zeiss LSM 800
Model
Light source
Detector
Objective
Filter set
Dichroic
Body
Olympus FV1000MPE-IX83 MP mi- croscope
Model
Light source
Detector
Objective
Filter set
Dichroic
Body
Zeiss LSM 710
Model
Light source
Detector
Objective
Filter set
Dichroic

Datasets

Dataset name
Organism
Gene / Protein
Dimensions
File size
SSBD:OMERO
Download
MovieS1_FoldingWing
Drosophila (NCBI:txid7215)
1024x1024x60x1x42
4.9GB
zip
MovieS2_CellExpand
Drosophila (NCBI:txid7215)
/ DE-cadherin (Q24298) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x44, 1024x1024x100x2x44
17.2GB, 17.2GB
zip
MovieS3_FoldingWing_sqhKO_Ant
Drosophila (NCBI:txid7215)
/ Myosin regulatory light chain sqh (P40423) / T-cell surface glycoprotein CD8 alpha chain (P01731)
512x512x100x2x57
5.6GB
zip
MovieS4_FoldingWing_sqhKO_Post
Drosophila (NCBI:txid7215)
/ Myosin regulatory light chain sqh (P40423) / T-cell surface glycoprotein CD8 alpha chain (P01731)
512x512x100x2x54
5.3GB
zip
MovieS5_Dpy_1
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
512x512x100x2x1
100MB
zip
MovieS5_Dpy_2
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
512x512x100x2x1
100MB
zip
MovieS6_Dpy
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x47, 1024x1024x100x2x47
18.4GB, 18.4GB
zip
MovieS7_DpyKD_dorsal
Drosophila (NCBI:txid7215)
Sb
1024x1024x55x1x34
3.7GB
zip
MovieS8_ctrl
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x50
19.5GB
zip
MovieS8_DpyOE
Drosophila (NCBI:txid7215)
Sb / SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x49
19.1GB
zip
MovieS9_ctrl
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x51
19.9GB
zip
MovieS9_DpyOE
Drosophila (NCBI:txid7215)
Sb / SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x47
18.4GB
zip
MovieS10_ctrl
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7)
1024x1024x100x2x49
19.1GB
zip
MovieS10_mutant
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7)
1024x1024x100x2x47
18.4GB
zip
MovieS11_DpyLocalize_1
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x85x2x23
2.1GB
zip
MovieS11_DpyLocalize_2
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x85x2x23
2.1GB
zip
MovieS12_ctrl
Drosophila (NCBI:txid7215)
/ SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x54
21.1GB
zip
MovieS12_NpKD
Drosophila (NCBI:txid7215)
Sb / SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x54
21.1GB
zip
MovieS12_SbKD
Drosophila (NCBI:txid7215)
Sb / SP460 (Q9U3W7) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x100x2x50
19.5GB
zip
FigS19_ctrl
Drosophila (NCBI:txid7215)
/ DE-cadherin (Q24298) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x150x2x17, 1024x1024x150x2x17
10GB, 10GB
zip
FigS19_DpyKD
Drosophila (NCBI:txid7215)
Sb / DE-cadherin (Q24298) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x150x2x19, 1024x1024x150x2x19
11.1GB, 11.1GB
zip
FigS19_NpKD
Drosophila (NCBI:txid7215)
Sb / DE-cadherin (Q24298) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x150x2x17, 1024x1024x150x2x19
10GB, 11.1GB
zip
FigS19_SbKD
Drosophila (NCBI:txid7215)
Sb / DE-cadherin (Q24298) / T-cell surface glycoprotein CD8 alpha chain (P01731)
1024x1024x150x2x17, 1024x1024x150x2x17
10GB, 10GB
zip