SSBD:database

479-Ikebe-MorphResDL/CP_20220910_P_0010

Author reviewed
Cell morphology of E. coli cells from the sensitive parental strain in the third dataset under phase contrast light microscopy at position 10
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Project
479-Ikebe-MorphResDL
Dataset ID
CP_20220910_P_0010
Kind
Image data
Title
Cell morphology of E. coli cells from the sensitive parental strain in the third dataset under phase contrast light microscopy at position 10
Description
In the third dataset, cell morphology of E. coli cells from the sensitive parental strain was observed and compared with that of CP-resistant E. coli cells using phase contrast light microscopy.
Folder Name
CP_20220910_P_0010
File Format
tif
File Size
29MB
File Separation Method
-
Figure Number
Figure1
Dimensions
2592x1944x1x3x1
X Scale
0.046 micrometer
Y Scale
0.046 micrometer
Z Scale
-
T Scale
-
Channel
3 channel
Quantitative Data
Yes

Biosample

Description
The parental MDS42 strain was cultured in the absence of antibiotics. Centrifuged and suspended bacterial cells were observed under phase contrast light microscopy.
Organism
Escherichia coli
(NCBITaxon:562)
Strain
Escherichia coli str. K-12 substr. MDS42 (https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1110693)
Cell
-
Cell line
-
MeSH
Escherichia coli (D004926)
Microscopy, Phase-Contrast (D008858)
Cell Growth Processes (D048708)
Cell Wall (D002473)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
cell growth (GO:0016049)
Cellular Component
cell envelope (GO:0030313)
cell wall (GO:0005618)
Molecular Function
-
MeSH Anatomy
Escherichia coli (D004926)
Microscopy, Phase-Contrast (D008858)
Cell Growth Processes (D048708)
Cell Wall (D002473)
MeSH BP
Escherichia coli (D004926)
Microscopy, Phase-Contrast (D008858)
Cell Growth Processes (D048708)
Cell Wall (D002473)
MeSH CC
Escherichia coli (D004926)
Microscopy, Phase-Contrast (D008858)
Cell Growth Processes (D048708)
Cell Wall (D002473)
MeSH MF
Escherichia coli (D004926)
Microscopy, Phase-Contrast (D008858)
Cell Growth Processes (D048708)
Cell Wall (D002473)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
phase contrast microscopy
(FBbi:00000247)
light microscopy
(FBbi:00000345)
Detection Method
wide-field detection
Visualization Method
-
Illumination Method
widefield illumination
Source of Contrast
differences in intrinsic optical density
Imaged Parameter
optical path length gradient
Sample Preparation Method
dispersed cells in vitro
MeSH
Escherichia coli (D004926)
Microscopy, Phase-Contrast (D008858)
Cell Growth Processes (D048708)
Cell Wall (D002473)

Instrument

Body
Leica DM750
Module
-
Light Source
Leica LED1
Detector
Leica ICC50 W/E
Objective
Leica HC PL FLUOTAR 100x/1,32 OIL PH3
Filter Set
-
Dichroic
-