SSBD:database

Project

Dataset ID

AMK_20220511_AMK2_0010

Kind

Image data

Title

Cell morphology of AMK-resistant E. coli cells from the second line of the resistant strain in the first dataset under phase contrast light microscopy at position 10

Description

In this study, the authors isolated four lines of Amikacin (AMK)-resistant Escherichia coli (E. coli). This dataset includes one of the image of the E. coli in the second line. In the first dataset, single colonies were isolated from AMK-resistant E. coli and cultured without antibiotics. Parts of AMK-resistant cells from the second line of the resistant strain showed slightly elongated morphology under phase contrast light microscopy, compared to the parental strain. The cell morphology showed difference among strains of four lines.

Folder Name

AMK_20220511_AMK2_0010

File Format

tif

File Size

29MB

File Separation Method

-

Figure Number

Figure1

Dimensions

2592x1944x1x3x1

X Scale

0.046 micrometer

Y Scale

0.046 micrometer

Z Scale

-

T Scale

-

Channel

3 channel

Quantitative Data

Yes

Description

Single colonies were isolated from the original AMK-resistant E. coli and cultured in the absence of antibiotics. Centrifuged and suspended bacterial cells were observed under phase contrast light microscopy.

Organism

Strain

-

Cell

-

Cell line

-

MeSH

Intrinsic Variable

-

Extrinsic Variable

-

Anatomical Entity

-

UBERON

-

Biological Process

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

wide-field detection

Visualization Method

-

Illumination Method

widefield illumination

Source of Contrast

differences in intrinsic optical density

Imaged Parameter

optical path length gradient

Sample Preparation Method

dispersed cells in vitro

MeSH

Body

Leica DM750

Module

-

Light Source

Leica LED1

Detector

Leica ICC50 W/E

Objective

Leica HC PL FLUOTAR 100x/1,32 OIL PH3

Filter Set

-

Dichroic

-