SSBD:database

472-Okuda-RamanFrozenIPSC/Fig3F_hiPSCsRaman_spectrum

Author reviewed
Raman spectrum data of frozen hiPSCs
SSBD:downloads
Project
472-Okuda-RamanFrozenIPSC
Dataset ID
Fig3F_hiPSCsRaman_spectrum
Kind
Image data
Title
Raman spectrum data of frozen hiPSCs
Description
Raman spectrum data of human induced pluripotent stem cells at -150 deg, the temperature commonly used for the long-term storage of cells. The spectrum includes Raman signals derived from DMSO, cytochromes, and lipid and protein. The peak areas of DMSO and cytochromes were normalized to the peak area of the CH signal at 1,447 cm^{-1}.
Folder Name
Fig3F_hiPSCsRaman_spectrum
File Format
.txt
File Size
26KB
File Separation Method
-
Figure Number
Fig3
Dimensions
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X Scale
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Y Scale
-
Z Scale
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T Scale
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Channel
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Quantitative Data
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Paper(s)

Biosample

Description
The frozen hiPSCs at -150 deg.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
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Cell line
hiPSC line, 1383D2
MeSH
Induced Pluripotent Stem Cells (D057026)
Spectrum Analysis, Raman (D013059)
Intrinsic Variable
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Extrinsic Variable
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Ontology

Anatomical Entity
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UBERON
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Biological Process
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Cellular Component
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Molecular Function
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MeSH Anatomy
Induced Pluripotent Stem Cells (D057026)
Spectrum Analysis, Raman (D013059)
MeSH BP
Induced Pluripotent Stem Cells (D057026)
Spectrum Analysis, Raman (D013059)
MeSH CC
Induced Pluripotent Stem Cells (D057026)
Spectrum Analysis, Raman (D013059)
MeSH MF
Induced Pluripotent Stem Cells (D057026)
Spectrum Analysis, Raman (D013059)

Genetics

Gene
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Protein / Probe
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Method / Genotype
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Treatments

Reagent / Compound
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Concentration
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Fold Dilution
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Imaging Method

Imaging Method
inelastic scattering of photons (Raman scattering) (FBbi:00000589)
Detection Method
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Visualization Method
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Illumination Method
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Source of Contrast
differences in chemical composition
Imaged Parameter
inelastic scattering of photons (Raman scattering)
Sample Preparation Method
living tissue
MeSH
Induced Pluripotent Stem Cells (D057026)
Spectrum Analysis, Raman (D013059)

Instrument

Body
custom-made slit-scanning Raman microscope
Module
Inverted microscope (Ti-E, Nikon)
Light Source
532 nm laser with continuous-wave oscillation (Millennia eV, Spectra Physics or Verdi 532 nm, Coherent)
Detector
CCD camera (PIXIS, 2048 B or PIXIS 400BReXcelon, Teledyne Princeton Instruments)
Objective
Nikon CFI Plan Apo Lambda 60XC
Filter Set
-
Dichroic
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