SSBD:database

472-Okuda-RamanFrozenIPSC/Fig3D_hiPSCsRaman_CS

Author reviewed
The numerical data of Raman image for CS stretching Raman shift obtained from frozen hiPSCs
SSBD:downloads
Project
472-Okuda-RamanFrozenIPSC
Dataset ID
Fig3D_hiPSCsRaman_CS
Kind
Image data
Title
The numerical data of Raman image for CS stretching Raman shift obtained from frozen hiPSCs
Description
The numerical data of Raman image for CS Raman shift, 673 cm^{-1}, which was obtained from frozen human induced pluripotent stem cells. The data was acquired at -150 deg, the temperature commonly used for the long-term storage of cells. This data show the location of DMSO. The image created from this numerical data is deposited in SSBD:repository.
Folder Name
Fig3D_hiPSCsRaman_CS
File Format
.txt
File Size
617KB
File Separation Method
-
Figure Number
Fig3
Dimensions
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X Scale
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Y Scale
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Z Scale
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T Scale
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Channel
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Quantitative Data
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Paper(s)

Biosample

Description
The frozen hiPSCs at -150 deg.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
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Cell
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Cell line
hiPSC line, 1383D2
MeSH
Induced Pluripotent Stem Cells (D057026)
Intrinsic Variable
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Extrinsic Variable
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Ontology

Anatomical Entity
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UBERON
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Biological Process
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Cellular Component
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Molecular Function
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MeSH Anatomy
Induced Pluripotent Stem Cells (D057026)
MeSH BP
Induced Pluripotent Stem Cells (D057026)
MeSH CC
Induced Pluripotent Stem Cells (D057026)
MeSH MF
Induced Pluripotent Stem Cells (D057026)

Genetics

Gene
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Protein / Probe
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Method / Genotype
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Treatments

Reagent / Compound
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Concentration
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Fold Dilution
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Imaging Method

Imaging Method
inelastic scattering of photons (Raman scattering) (FBbi:00000589)
Detection Method
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Visualization Method
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Illumination Method
-
Source of Contrast
differences in chemical composition
Imaged Parameter
inelastic scattering of photons (Raman scattering)
Sample Preparation Method
living tissue
MeSH
Induced Pluripotent Stem Cells (D057026)

Instrument

Body
custom-made slit-scanning Raman microscope
Module
Inverted microscope (Ti-E, Nikon)
Light Source
532 nm laser with continuous-wave oscillation (Millennia eV, Spectra Physics or Verdi 532 nm, Coherent)
Detector
CCD camera (PIXIS, 2048 B or PIXIS 400BReXcelon, Teledyne Princeton Instruments)
Objective
Nikon CFI Plan Apo Lambda 60XC
Filter Set
-
Dichroic
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