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460-Tanaka-RadialAxisDyn/VideoS3_2 thumbnail

460-Tanaka-RadialAxisDyn/VideoS3_2

Author reviewed
The time-lapse images of living dynamics from the zygote to 2-cell stage embryo in WT Arabidopsis
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Project
460-Tanaka-RadialAxisDyn
Dataset ID
VideoS3_2
Kind
Image data
Title
The time-lapse images of living dynamics from the zygote to 2-cell stage embryo in WT Arabidopsis
Description
The marker WOX2p::H2B-GFP to label the embryo nucleus and the marker WOX2p::LTI-tdTomato to label the plasma membrane (PM) of Arabidopsis showed the living dynamics from the zygote to 2-cell stage embryo in vitro-cultivated WT at 10-min intervals.
Folder Name
VideoS3_2
File Format
.tif
File Size
773.2MB
File Separation Method
-
Figure Number
VideoS3
Dimensions
512x512x11x2x67
X Scale
2.0 micrometer
Y Scale
2.0 micrometer
Z Scale
5.0 micrometer
T Scale
10 minutes
Channel
2 channel
Quantitative Data
-

Biosample

Description
The embryo nucleus of Arabidopsis was labelld by the marker WOX2p::H2B-GFP. The plasma membrane (PM) of Arabidopsis was labelled by the marker WOX2p::LTI-tdTomato.
Organism
Arabidopsis thaliana
(NCBITaxon:3702)
Strain
Col-0 (https://abrc.osu.edu/stocks/1)
Cell
zygote (CL:0010017)
Cell line
-
MeSH
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Membrane (D002462)
Intrinsic Variable
The marker WOX2p::H2B-GFP was to label the embryo nucleus, and the marker WOX2p::LTI-tdTomato was to label the plasma membrane (PM) of Arabidopsis.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
zygote stage (UBERON:0000106)
2-cell stage embryo (UBERON:0019249)
Biological Process
periclinal cell division (GO:0090511)
Cellular Component
nucleus (GO:0005634)
plasma membrane (GO:0005886)
Molecular Function
-
MeSH Anatomy
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Membrane (D002462)
MeSH BP
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Membrane (D002462)
MeSH CC
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Membrane (D002462)
MeSH MF
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Membrane (D002462)

Genetics

Gene
-
Protein / Probe
H2B
Method / Genotype
transgenic
Gene
-
Protein / Probe
Hydrophobic protein RCI2B
Method / Genotype
transgenic

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
spinning disk confocal microscopy
(FBbi:00000253)
Detection Method
spot detector
Visualization Method
Green fluorescent protein from Aequorea tdTomatoFP
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Embryonic Structures (D004628)
Cell Membrane (D002462)

Instrument

Body
Yokogawa Electric CSU-W1
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-