77-Aoki-CellDynERK
Author reviewedTime-lapse imgaes and quantitative information of propagating wave of ERK activation orients collective cell migration
Project ID
77-Aoki-CellDynERK
Title
Time-lapse imgaes and quantitative information of propagating wave of ERK activation orients collective cell migration
Description
-
Release Date
2018-11-14
Update Date
2022-11-21
License
Version
1.0.0
Method Summary
See details in Aoki K, et. al. (2017) Dev Cell, Nov 6;43(3):305-317.e5.
Kind
Image data
Quantitative data
Total
37 files / 1.7 GB
Image datasets
zipped 15 files / 336.5 MB, raw image 19 files / 1.4 GB, total 34 files / 1.7 GB
Quantitative datasets
zipped 1 files / 34.7 KB, raw bdml 2 files / 548.0 KB, total 3 files / 582.8 KB
Organism
Canis lupus familiaris
(
NCBITaxon:9615
)
Strain
-
Cell line
MDCK cell
(
CLO:0007646
)
Biological Process
activation of MAP ERK kinase kinase
(
GO:0000186
)
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
time lapse microscopy
(
FBbi:00000249
)
Image datasets
14
Quantitative datasets
1
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Aoki Kazuhiro
National Institutes of Natural Sciences
National Institute for Basic Biology
Division of Quantitative Biology
5-1, Higashiyama, Myodaiji, Okazaki, Aichi 444-8787 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
Aoki Kazuhiro
(National Institutes of Natural Sciences)
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of ERK activity during the wound-healing process in MDCK cells expressing EKAREV-NLS.
Time-lapse images of the clockwise rotation of ERK activation on a circular-patterned substrate and counterclockwise rotation of cell migration during the wound-healing process in MDCK cells expressing EKAREV-NLS.
Time-lapse images of ERK activation induce collective cell migration through the regulation of the MLC.
Time-lapse images of the spontaneous wave of ERK activation in MDCK cells expressing EKAREV-NLS and MCL-mCherry.
Time-lapse images of the accumulations of MCL-mCherry in MDCK cells expressing EKAREV-NLS and MCL-mCherry.
Time-lapse images of the spontaneous wave of ERK activation and the accumulation of MCL-mCherry.
BDML file for quantiative information about MLC-mCherry accumulation in MDCK cells expressing EKAREV-NLS and MLC-mCherry.
Time-lapse images of the spontaneous wave of ERK activation in MDCK cells expressing EKAREV-NLS.
Time-lapse images of the H2B-iRFP in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Time-lapse images of the mCherry-ERK in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Montage images of the optic flow of cell migration in MDCK cells expresssing CRY2-CRaf, CIBN-EGFP-KRasCT, FLAG-MEK-mCFP, mCherry-ERK, and histone H2B-iRFP.
Time-lapse epifluorescence images of spontaneous waves in MDCK cells expressing EKAREV-NLS with the presence of ADAM10 inhibitor.
Time-lapse epifluorescence images of spontaneous waves in MDCK cells expressing EKAREV-NLS treated with DMSO.
Time-lapse epifluorescence images of light-induced ERK acivation in MDCK cells expressing EKAREV-NLS co-cultured with MDCK cells expressing CRY2-CRaf, CIBN-EGFP-KRasCT, and H2B-iRFP.
Time-lapse epifluorescence images of light-induced ERK acivation in MDCK cells expressing EKAREV-NLS co-cultured with MDCK cells expressing CRY2-CRaf, CIBN-EGFP-KRasCT, and H2B-iRFP.