70-Yoshida-KTMTDyn
Author reviewedTimelapse confocal images and BDML files for quantitative information about the kinetochore microtubule dynamics during mouse oocytes meiosis
Project ID
70-Yoshida-KTMTDyn
Title
Timelapse confocal images and BDML files for quantitative information about the kinetochore microtubule dynamics during mouse oocytes meiosis
Description
-
Release Date
2021-09-30
Update Date
-
License
Version
1.0.0
Method Summary
See details in Yoshida S, et. al. (2015) Dev Cell., 33(5):589-602.
Kind
Image data
Total
73406 files / 611.9 GB
Image datasets
zipped 265 files / 240.4 GB, raw image 73141 files / 371.5 GB, total 73406 files / 611.9 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
-
Cell line
-
Biological Process
mitotic cell cycle
(
GO:0007067
)
Cellular Component
kinetochore microtubule
(
GO:0005828
)
chromosome
(
GO:0005694
)
Medical Subject Headings
Animals
(
MeSH:D000818
)
Aurora Kinase B
(
MeSH:D064107
)
Aurora Kinase B/metabolism
(
MeSH:Q000378
)
Aurora Kinase C
(
MeSH:D064108
)
Aurora Kinase C/metabolism
(
MeSH:Q000378
)
CDC2 Protein Kinase
(
MeSH:D016203
)
CDC2 Protein Kinase/metabolism
(
MeSH:Q000378
)
Cell Cycle Proteins
(
MeSH:D018797
)
Cell Cycle Proteins/metabolism
(
MeSH:Q000378
)
Chromosome Segregation
(
MeSH:D020090
)
Female
(
MeSH:D005260
)
Fluorescent Antibody Technique
(
MeSH:D005455
)
Image Processing
Computer-Assisted
(
MeSH:D007091
)
Kinetochores
(
MeSH:D018386
)
Kinetochores/physiology
(
MeSH:Q000502
)
Meiosis
(
MeSH:D008540
)
Meiosis/physiology
(
MeSH:Q000502
)
Mice
(
MeSH:D051379
)
Microtubules
(
MeSH:D008870
)
Microtubules/physiology
(
MeSH:Q000502
)
Oocytes
(
MeSH:D009865
)
Oocytes/cytology
(
MeSH:Q000166
)
Oocytes/metabolism
(
MeSH:Q000378
)
Phosphorylation
(
MeSH:D010766
)
Protein Phosphatase 2
(
MeSH:D054648
)
Protein Phosphatase 2/metabolism
(
MeSH:Q000378
)
Protein-Serine-Threonine Kinases
(
MeSH:D017346
)
Protein-Serine-Threonine Kinases/metabolism
(
MeSH:Q000378
)
Method involved in biological imaging
time lapse microscopy
(
FBbi:00000249
)
fluorescence microscopy
(
FBbi:00000246
)
Image datasets
265
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Tomoya S. Kitajima
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Chromosome Segregation
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
Tomoya S. Kitajima
(RIKEN)
Shuhei Yoshida
(RIKEN)
Masako Kaido
(RIKEN)
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 3 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 60 minutes (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 2 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 4 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytesduring MI for 5 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 2 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 4 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 5 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Timelapse confocal images of mEGFP-INCENP expressing oocytes at the end of the stretching phase (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of mEGFP-INCENP expressing oocytes during the stabilization phase (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of mEGFP-INCENP expressing oocytes at meiosis II (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of mEGFP-INCENP expressing oocytes at meiosis II (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of Hec1-mEGFP-CENP-C expressing oocytes during meiosis I for 2 hours (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of Hec1-mEGFP-CENP-C expressing oocytes during meiosis I for 4 hours (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of Hec1-mEGFP-CENP-C expressing oocytes during meiosis I for 6 hours (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of Hec1-mEGFP-CENP-C expressing oocytes during meiosis II (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of the metaphase plate of the meiosis I/MII fused mEGFP-hINCENP expressing oocytes (stained for INCENP: green, CENP-C: red, chromosomes: blue )
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of the metaphase plate of the meiosis I/MII fused mEGFP-Hec1 expressing oocytes (stained for INCENP: green, CENP-C: red, chromosomes: blue )
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MII for 2 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 4 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 6 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MII (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 2 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 4 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 6 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of pS24-Knl1 expressing oocytes during meiosis MI for 2 hours (stained for pS24-knl1: green, KT: red, chromosomes: bulue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of pS24-knl1 expressing oocytes during meiosis MI for 4 hours (stained for pS24-knl1: green, KT: red, chromosomes: bulue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of pS24-knl1 expressing oocytes during meiosis MI for 6 hours (stained for pS24-knl1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of pS24-knl1 expressing oocytes during meiosis MII (stained for pS24-knl1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 1 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 2 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 3 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 4 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 5 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 6 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MII (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of pS55-Hec1 level of KTs in fused Hec1 expressing oocytes (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of oocytes epressing 3mEGFP-PP22A-B56e (green) and H2B-mCherry (red) cultured in control medium
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of oocytes epressing 3mEGFP-PP22A-B56e (green) and H2B-mCherry (red) cultured in the treatment with the Eg5 inhibitor monastrol (right)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of oocytes epressing 3mEGFP-PP22A-B56e (green) and H2B-mCherry (red) cultured in the control (left) or treatment with the Eg5 inhibitor monastrol (right)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of oocytes epressing 3mEGFP-PP22A-B56e (green) and H2B-mCherry (red) cultured in the treatment with the Eg5 inhibitor monastrol (right)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 120 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 180 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 240 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 300 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 360 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 60 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e culutured in the treatment with the Eg5 inhibitor monastrol for 120 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e culutured in the treatment with the Eg5 inhibitor monastrol for 240 minutes (choromosome: blue)