233-Nishiyama-CellDyn
Author reviewedLive imaging of HEK293 cells expressing P2Y6 receptors or P2Y6R mutants.
Project ID
233-Nishiyama-CellDyn
Title
Live imaging of HEK293 cells expressing P2Y6 receptors or P2Y6R mutants.
Description
-
Release Date
2022-11-23
Update Date
-
License
Version
1.0.0
Method Summary
See details in Nishiyama K, et. al. (2022) Sci Signal, Jan 11;15(716):eabj0644.
Kind
Image data
Total
109 files / 153.3 MB
Image datasets
zipped 41 files / 62.4 MB, raw image 68 files / 90.9 MB, total 109 files / 153.3 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell line
HEK293
(
CLO:0001230
)
Biological Process
regulation of G protein-coupled receptor internalization
(
GO:1904020
)
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
fluorescence microscopy
(
FBbi:00000246
)
Image datasets
41
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Motohiro Nishida
Kyushu University
Graduate School of Pharmaceutical Sciences
-
Kyushu University, Fukuoka 812-8582, Japan.
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Live imaging of SFN-induced P2Y6R-YFP internalization in HEK293 cells at time point of 0min.
Live imaging of SFN-induced P2Y6R-YFP internalization in HEK293 cells at time point of 180min.
Live imaging of SFN-induced P2Y6R-YFP internalization in HEK293 cells at time point of 60min.
Live imaging of HEK293 cells coexpressing P2Y6R C220S-YFP and Lifeact at time point of 0min.
Live imaging of HEK293 cells coexpressing P2Y6R C220S-YFP and Lifeact at time point of 180min.
Live imaging of HEK293 cells coexpressing P2Y6R C220S-YFP and Lifeact at time point of 60min.
Live imaging of HEK293 cells coexpressing P2Y6R-YFP and Lifeact at time point of 0min.
Live imaging of HEK293 cells coexpressing P2Y6R-YFP and Lifeact at time point of 120min.
Live imaging of HEK293 cells coexpressing P2Y6R-YFP and Lifeact at time point of 60min.
Live imaging of HEK293 cells coexpressing Lifeact and P2Y6R (WT)–YFP at time point of 0min.
Live imaging of HEK293 cells coexpressing Lifeact and P2Y6R (WT)–YFP at time point of 30min.
Live imaging of HEK293 cells coexpressing Lifeact and P2Y6R (WT)–YFP at time point of 60min.
Live imaging of HEK293 cells coexpressing Lifeact and P2Y6R (C220S)–YFPat time point of 0min.
Live imaging of HEK293 cells coexpressing Lifeact and P2Y6R (C220S)–YFPat time point of 30min.
Live imaging of HEK293 cells coexpressing Lifeact and P2Y6R (C220S)–YFP at time point of 60min.
Live imaging of HEK293 cells coexpressing AT1R-YFP and Lifeact incubated with Ang II at time point of 0min.
Live imaging of HEK293 cells coexpressing AT1R-YFP and Lifeact incubated with Ang II at time point of 15min.
Live imaging of HEK293 cells coexpressing AT1R-YFP and Lifeact incubated with Ang II at time point of 5min.
Live imaging of HEK293 cells coexpressing AT1R-YFP and Lifeact incubated with SFN at time point of 0min..
Live imaging of HEK293 cells coexpressing AT1R-YFP and Lifeact incubated with SFN at time point of 180min.
Live imaging of HEK293 cells coexpressing AT1R-YFP and Lifeact incubated with SFN at time point of 60min.
Live imaging of HEK293 cells coexpressing A2BAR-EGFP and Lifeact and incubated with SFN at time point of 0min.
Live imaging of HEK293 cells coexpressing A2BAR-EGFP and Lifeact and incubated with SFN at time point of 180min.
Live imaging of HEK293 cells coexpressing A2BAR-EGFP and Lifeact and incubated with SFN at time point of 60min.
Live imaging of SFN effect on subcellular distribution of b-arrestin incubated with SFN at the time point of 30min.
Live imaging of SFN effect on subcellular distribution of b-arrestin without SFN (control).
Live imaging of SFN effect on subcellular distribution of b-arrestin incubated with SFN at the time point of 180min.
Live imaging of HEK293 cells expressing P2Y6R mutant (C119S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C129S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C148S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C159S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C177S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C18S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C211-213S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C220S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C273S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C46S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C52S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C77S) treated with UDP.
Live imaging of HEK293 cells expressing P2Y6R mutant (C99S) treated with UDP.
Live imaging of HEK293 cells expressing WT-P2Y6R treated with UDP.