228-Kanadome-CellInteration thumbnail

228-Kanadome-CellInteration

Author reviewed
Time-lapse images of FRET‐based indicators dynamics in HEK293T cells or K562 cells.
Project ID
228-Kanadome-CellInteration
Title
Time-lapse images of FRET‐based indicators dynamics in HEK293T cells or K562 cells.
Description
-
Release Date
2022-11-23
Update Date
-
License
Version
1.0.0
Method Summary
See details in Kanadome T, et. al. (2021) Sci Rep, 2021 Nov 15;11(1):22237.
Paper DOI
Paper URL
Kind
Image data
Total
174 files / 1.7 GB
Image datasets
zipped 8 files / 544.4 MB, raw image 166 files / 1.1 GB, total 174 files / 1.7 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism
Homo sapiens ( NCBITaxon:9606 )
Strain
-
Cell line
HEK293T ( CLO:0037271 )
K562 cell
Biological Process
homophilic cell adhesion via plasma membrane adhesion molecules ( GO:0007156 )
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
time lapse microscopy ( FBbi:00000249 )
FRET ( FBbi:00000367 )

Image datasets
8
Quantitative datasets
0

Paper DOI / Paper URL

OMERO.gallery
OMERO
downloads

People
Contact
Tomoki Matsuda
Osaka University
Department of Biomolecular Science and Engineering
-
8-1 Mihogaoka, Ibaraki 567-0047, Japan
Takeshi Yagi
Osaka University
Graduate School of Frontier Biosciences
Laboratories for Integrated Biology
Suita 565-0871, Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-

Datasets

Thumbnail
Dataset
Organism
Kind / Links
Time-lapse imaging of the post-optimized FRET-based γB2ΔICD indicators in HEK293T cells.
Time-lapse imaging of the pre-optimized FRET-based γB2ΔICD indicators in HEK293T cells.
Time-lapse imaging of the FRET efficiency of γB2–γB2 by acceptor photobleaching in HEK293T cells.
Time-lapse imaging of the FRET efficiency of γB2–γB2γA3 by acceptor photobleaching in HEK293T cells.
Time-lapse imaging of the FRET efficiency of γB2γA3–γB2 by acceptor photobleaching in HEK293T cells.
Time-lapse imaging of the FRET efficiency of γB2γA3–γB2γA3 by acceptor photobleaching in HEK293T cells.
Time-lapse imaging of the effect of Ca2+ chelation by EGTA in K562 cells.
Time-lapse imaging of the effect of Ca2+ chelation before and after EGTA treatment in K562 cells.