213-Yoshida-SpindleDyn
Author reviewedLive imaging of spindle bipolarization during meiosis I in mouse oocytes.
Project ID
213-Yoshida-SpindleDyn
Title
Live imaging of spindle bipolarization during meiosis I in mouse oocytes.
Description
-
Release Date
2022-03-31
Update Date
-
License
Version
1.0.0
Method Summary
See details in Yoshida S, et. al. (2020) Nat Commun., 11(1):2652.
Kind
Image data
Total
2538 files / 69.2 GB
Image datasets
zipped 16 files / 26.2 GB, raw image 2522 files / 43.0 GB, total 2538 files / 69.2 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
-
Cell line
-
Biological Process
meiosis I nuclear membrane disassembly
(
GO:0051079
)
Cellular Component
Medical Subject Headings
Animals
(
MeSH:D000818
)
Cell Cycle Proteins
(
MeSH:D018797
)
Cell Cycle Proteins/metabolism
(
MeSH:Q000378
)
Chromosome Segregation
(
MeSH:D020090
)
Cytoskeletal Proteins
(
MeSH:D003598
)
Cytoskeletal Proteins/metabolism
(
MeSH:Q000378
)
Female
(
MeSH:D005260
)
Gametogenesis
(
MeSH:D005718
)
Gametogenesis/physiology
(
MeSH:Q000502
)
Kinetochores
(
MeSH:D018386
)
Kinetochores/metabolism
(
MeSH:Q000378
)
Meiosis
(
MeSH:D008540
)
Meiosis/physiology
(
MeSH:Q000502
)
Mice
(
MeSH:D051379
)
Microtubule-Associated Proteins
(
MeSH:D008869
)
Microtubule-Associated Proteins/metabolism
(
MeSH:Q000378
)
Microtubules
(
MeSH:D008870
)
Microtubules/metabolism
(
MeSH:Q000378
)
Nuclear Proteins
(
MeSH:D009687
)
Nuclear Proteins/metabolism
(
MeSH:Q000378
)
Oocytes
(
MeSH:D009865
)
Oocytes/metabolism
(
MeSH:Q000378
)
Spindle Apparatus
(
MeSH:D008941
)
Spindle Apparatus/metabolism
(
MeSH:Q000378
)
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
time lapse microscopy
(
FBbi:00000249
)
Image datasets
16
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Tomoya S. Kitajima
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Chromosome Segregation
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Live imaging of Ndc80f/f (control) oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta).
Live imaging of Ndc80f/f Zp3-Cre (Ndc80-deleted) oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta).
Live imaging of Ndc80f/f Zp3-Cre oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta).
Mus musculus
(
NCBITaxon:10090
)
Live imaging of Ndc80f/f Zp3-Cre oocytes expressing full-length Ndc80 (Ndc80-WT) monitored for spindle formation.
Mus musculus
(
NCBITaxon:10090
)
Live imaging of Ndc80f/f Zp3-Cre oocytes coexpressing Ndc80ΔN and Nuf2ΔN (Ndc80ΔN/ Nuf2ΔN) monitored for spindle formation.
Live imaging of Ndc80f/f oocytes labeled with EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta) after Prc1 RNAi.
Live imaging of Ndc80f/f oocytes labeled with EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta).
Live imaging of dynamic exchange of Prc1 at kinetochores in mouse oocytes.
Live imaging of fused Ndc80f/f Zp3-Cre oocyte in metaphase II (MII) and an Ndc80f/f Zp3-Cre oocyte at the GV stage (MI).
Live imaging of control oocytes labeled with EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta).
Live imaging of oocytes expressing increased levels of Prc1 labeled with EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta).
Live imaging of Cyclin B2 overexpression Ndc80f/f oocytes labeled with EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta).
Live imaging of Cyclin B2 overexpression Ndc80f/f Zp3-Cre oocytes labeled with EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta).
Live imaging of oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta) as control.
Live imaging of oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta) after Prc1 RNAi without expression of siRNA-resistant Prc1.
Live imaging of oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta) after Prc1 RNAi with expression of siRNA-resistant Prc1.