211-Hiroshima-EGFRTracking
Author reviewedSingle-molecule imaging and BDML file for quantitative information about trajectories of EGFR dynamics and activities in living CHO-K1 cells.
Project ID
211-Hiroshima-EGFRTracking
Title
Single-molecule imaging and BDML file for quantitative information about trajectories of EGFR dynamics and activities in living CHO-K1 cells.
Description
-
Release Date
2022-03-31
Update Date
-
License
Version
1.0.0
Method Summary
See details in Hiroshima M, et. al. (2018) J Mol Biol., 430(9):1386-1401.
Kind
Image data
Total
171 files / 53.3 GB
Image datasets
zipped 12 files / 22.0 GB, raw image 159 files / 31.3 GB, total 171 files / 53.3 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Cricetulus griseus
(
NCBITaxon:10029
)
Strain
-
Cell line
CHO-K1
(
CLO:0002462
)
Biological Process
epidermal growth factor receptor signaling pathway
(
GO:0007173
)
Cellular Component
Grb2-EGFR complex
(
GO:0070436
)
Medical Subject Headings
Animals
(
MeSH:D000818
)
CHO Cells
(
MeSH:D016466
)
Cluster Analysis
(
MeSH:D016000
)
Cricetulus
(
MeSH:D003412
)
Cytoplasm
(
MeSH:D003593
)
Cytoplasm/metabolism
(
MeSH:Q000378
)
Epidermal Growth Factor
(
MeSH:D004815
)
Epidermal Growth Factor/metabolism
(
MeSH:Q000378
)
ErbB Receptors
(
MeSH:D066246
)
ErbB Receptors/chemistry
(
MeSH:Q000737
)
ErbB Receptors/metabolism
(
MeSH:Q000378
)
Fluorescent Antibody Technique
(
MeSH:D005455
)
GRB2 Adaptor Protein
(
MeSH:D051380
)
GRB2 Adaptor Protein/metabolism
(
MeSH:Q000378
)
Signal Transduction
(
MeSH:D015398
)
Single Molecule Imaging
(
MeSH:D000072760
)
Single Molecule Imaging/methods
(
MeSH:Q000379
)
Spatio-Temporal Analysis
(
MeSH:D062211
)
Method involved in biological imaging
evanescent wave microscopy(total internal reflection fluorescence microscopy)
(
FBbi:00000617
)
time lapse microscopy
(
FBbi:00000249
)
Image datasets
12
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Yasushi Sako
RIKEN
-
Cellular Informatics Laboratory
2-1 Hirosawa, Wako 351-0198, Japan
Imaging dataset contributor
-
Quantitative dataset contributor
Michio Hiroshima
(RIKEN)
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Single-molecule imaging of trajectories of EGFR–GFP molecules in the basal membrane of living cells.
Single-molecule imaging of trajectories of EGFR clusters for 2.4 s before stimulation.
Single-molecule imaging of trajectories of EGFR clusters for 2.4 s before stimulation.
Single-molecule imaging of trajectories of EGFR clusters for 2.4 s before stimulation.
Single-molecule imaging of trajectories of EGFR clusters for 2.4 s before stimulation.
Single-molecule imaging of trajectories of EGFR clusters for 2.4 s after 30nM EGF stimulation for 60s.
Single-molecule imaging of trajectories of EGFR clusters for 2.4 s after 4nM EGF stimulation for 15s.
Single-molecule imaging of trajectories of EGFR clusters for 2.4 s after 4nM EGF stimulation for 30s.
Single-molecule imaging of trajectories of EGFR clusters for 2.4 s after 4nM EGF stimulation for 60s.
Single-molecule imaging of trajectories of the dynamics and activity of Arm mutant EGFR.
Single-molecule imaging of trajectories of the dynamics and activity of ICD mutant EGFR.
Time-lapse of single-molecule imaging of EGFinduced GRB2–HaloTag::TMR translocation from cytoplasm to the basal plasma membrane.