203-Tanabe-SingleDyn
Author reviewedImages of signaling molecules dynamic in Dictyostelium discoideum cells.
Project ID
203-Tanabe-SingleDyn
Title
Images of signaling molecules dynamic in Dictyostelium discoideum cells.
Description
-
Release Date
2022-03-31
Update Date
-
License
Version
1.0.0
Method Summary
See details in Tanabe Y, et. al. (2018) J Cell Sci., 131(23):jcs214775.
Paper DOI
Kind
Image data
Organism
Dictyostelium discoideum
(
NCBITaxon:44689
)
Strain
Dictyostelium discoideum AX2
(
NCBITaxon:366501
)
gcΔ cell
Cell line
-
Biological Process
chemotaxis
(
GO:0006935
)
Cellular Component
-
Medical Subject Headings
Animals
(
MeSH:D000818
)
Chemotaxis
(
MeSH:D002633
)
Chemotaxis/physiology
(
MeSH:Q000502
)
Eukaryota
(
MeSH:D056890
)
Eukaryota/metabolism
(
MeSH:Q000378
)
Signal Transduction
(
MeSH:D015398
)
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
Image datasets
11
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Masahiro Ueda
Osaka University
Graduate School of Frontier Biosciences
Laboratory for Cell Signaling Dynamics
6-2-3, Furuedai, Suita, Osaka 565-0874 JAPAN
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Images of sGC and PIP3 in a wild-type AX2 cell expressing sGCN–TMR and PHPKB–GFP in the membrane.
Images of signaling molecules dynamics in AX2 cells exposed to a cAMP gradients during chemotaxis.
Images of a gcΔ cell expressing sGC–GFP was stimulated upon 2-s pulse inputs of cAMP.
Images of a gcΔ cell expressing sGC–GFP was stimulated upon 100-nM step inputs of cAMP.
Imges of a gcΔ cell expressing sGC–GFP stimulated via 0.5 sec UV exposure.
Imges of a gcΔ cell expressing sGC–GFP stimulated via 1.5 sec UV exposure.
Imges of a gcΔ cell spontaneously expressing sGC–GFP.
Dictyostelium discoideum
(
NCBITaxon:44689
)
Images of a wild-type AX2 cell co-expressing mRFP–LimEΔcoil and sGC–GFP treated with 1 μM LatA.
Dictyostelium discoideum
(
NCBITaxon:44689
)
Images of a wild-type AX2 cell co-expressing PHPKB–GFP and sGCN–TMR stimulated by repetitive pulse stimuli.
Images of gcΔ cells expressing sGCWT–GFP stimulated with 100 nM cAMP.
Images of gcΔ cells expressing sGCΔcat–GFP stimulated with 100 nM cAMP.