194-Obashi-SpineDyn
Author reviewedMicroscopy images or BDML file for quantiative information about actin polymers mobility in mouse dendritic spines.
Project ID
194-Obashi-SpineDyn
Title
Microscopy images or BDML file for quantiative information about actin polymers mobility in mouse dendritic spines.
Description
-
Release Date
2022-03-31
Update Date
-
License
Version
1.0.0
Method Summary
See details in Obashi K, et. al. (2019) Cell Rep., 27(5):1503-1515.
Kind
Image data
Total
42 files / 15.3 MB
Image datasets
zipped 21 files / 5.9 MB, raw image 21 files / 9.4 MB, total 42 files / 15.3 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
-
Cell line
-
Biological Process
actin polymerization
(
GO:0030041
)
stimulation of synapse structural plasticity
(
GO:0051835
)
Cellular Component
dendritic spine
(
GO:0043197
)
Medical Subject Headings
Actin Cytoskeleton
(
MeSH:D008841
)
Actin Cytoskeleton/metabolism
(
MeSH:Q000378
)
Actins
(
MeSH:D000199
)
Actins/metabolism
(
MeSH:Q000378
)
Animals
(
MeSH:D000818
)
Biological Transport
(
MeSH:D001692
)
Calcium-Calmodulin-Dependent Protein Kinase Type 2
(
MeSH:D054732
)
Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
(
MeSH:Q000378
)
Cells
Cultured
(
MeSH:D002478
)
Dendritic Spines
(
MeSH:D049229
)
Dendritic Spines/metabolism
(
MeSH:Q000378
)
Dendritic Spines/ultrastructure
(
MeSH:Q000648
)
Diffusion
(
MeSH:D004058
)
Female
(
MeSH:D005260
)
Male
(
MeSH:D008297
)
Mice
(
MeSH:D051379
)
Mice
Inbred ICR
(
MeSH:D008813
)
Neuronal Plasticity
(
MeSH:D009473
)
Signal Transduction
(
MeSH:D015398
)
T-Lymphoma Invasion and Metastasis-inducing Protein 1
(
MeSH:D000075684
)
T-Lymphoma Invasion and Metastasis-inducing Protein
1/metabolism
(
MeSH:Q000378
)
Method involved in biological imaging
two-photon laser scanning microscopy
(
FBbi: 00000254
)
time lapse microscopy
(
FBbi:00000249
)
structured illumination microscopy
(
FBbi:00000332
)
Image datasets
21
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Shigeo Okabe
the University of Tokyo, Tokyo
Department of Cellular Neurobiology, Graduate School of Medicine
-
7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
Kazuki Obashi
(the University of Tokyo, Tokyo)
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in mouse spine structural without glutamate induction.
Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in mouse spine structural plasticity induced by repetitive glutamate
Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in knockdown of ADF/cofilin one.
Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in control one.
Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in knockdown of ADF/cofilin one after treated with shRNA-resistant cofilin.
Time-lapse 2D SIM images of an EGFP-expressing neuron during uncaging-induced sLTP at 22 DIV.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing CaMKIIa-EGFP and mCherry during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing CaMKIIa-EGFP and mCherry during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing Tiam1QK-EGFP and mCherry with Control-sh during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing Tiam1QK-EGFP and mCherry with Control-sh during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing Tiam1QK-deltaPHnCCEx-EGFP and mCherry with Control-sh during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing Tiam1QK-deltaPHnCCEx-EGFP and mCherry with Control-sh during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing Tiam1QK-EGFP and mCherry with sh-ADF/cofilin during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing Tiam1QK-EGFP and mCherry with sh-ADF/cofilin during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing cofilin-EGFP and mCherry during uncaging-induced sLTP.
Time-lapse images for cultured hippocampal neurons at 12–15 DIV expressing cofilin-EGFP and mCherry during uncaging-induced sLTP.
2D SIM image of glutamate-uncaging-induced spine neurons expressing EGFP and mRFP at 13–19 DIV.
2D SIM image of glutamate-uncaging-induced spine neurons expressing Tiam1QK-EGFP and mRFP at 13–19 DIV.
2D SIM image of spine neurons at 13–19 DIV expressing Tiam1QK-EGFP and mRFP before glutamate-uncaging-induced.
2D SIM image of glutamate-uncaging-induced spine neurons expressing Tiam1QK-deltaPHnCCEx-EGFP and mRFP at 13–19 DIV.
2D SIM image of spine neurons at 13–19 DIV expressing Tiam1QK-deltaPHnCCEx-EGFP and mRFP before glutamate-uncaging-induced.