188-Ipponjima-NHEKsCornification thumbnail

188-Ipponjima-NHEKsCornification

Author reviewed
Images of morphological changes in granular cells during cornification.
Project ID
188-Ipponjima-NHEKsCornification
Title
Images of morphological changes in granular cells during cornification.
Description
-
Release Date
2022-03-31
Update Date
-
License
Version
1.0.0
Method Summary
See details in Ipponjima S, et. al. (2020) Sci Rep., 10(1):5515.
Paper DOI
Paper URL
Kind
Image data
Total
42 files / 426.3 GB
Image datasets
zipped 21 files / 161.0 GB, raw image 21 files / 265.3 GB, total 42 files / 426.3 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism
Homo sapiens ( NCBITaxon:9606 )
Strain
-
Cell line
-
Biological Process
keratinocyte activation ( GO:0032980 )
mitochondrial depolarization ( GO:0051882 )
Cellular Component
nucleus ( GO:0005634 )
Medical Subject Headings
Cell Shape ( MeSH:D048430 )
Cells
Cultured ( MeSH:D002478 )
Humans ( MeSH:D006801 )
Keratinocytes ( MeSH:D015603 )
Keratinocytes/cytology ( MeSH:Q000166 )
Keratinocytes/metabolism ( MeSH:Q000378 )
Keratinocytes/ultrastructure ( MeSH:Q000648 )
Microscopy ( MeSH:D008853 )
Models
Biological ( MeSH:D008954 )
Organelles ( MeSH:D015388 )
Organelles/metabolism ( MeSH:Q000378 )
Organelles/ultrastructure ( MeSH:Q000648 )
S100 Proteins ( MeSH:D009418 )
S100 Proteins/metabolism ( MeSH:Q000378 )
Time-Lapse Imaging ( MeSH:D059008 )
Time-Lapse Imaging/methods ( MeSH:Q000379 )
Method involved in biological imaging
two-photon laser scanning microscopy ( FBbi:00000254 )
time lapse microscopy ( FBbi:00000249 )

Image datasets
21
Quantitative datasets
0

Paper DOI / Paper URL

OMERO.gallery
OMERO
downloads

People
Contact
Sari Ipponjima
Hokkaido University
Research Center of Mathematics for Social Creativity, Research Institute for Electronic Science
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Sapporo Kita 20 Nishi 10, 001-0020, Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-

Datasets

Thumbnail
Dataset
Organism
Kind / Links
Images of an epidermal model labelled with Hoechst 33258 and CTG in human epidermal keratinocytes (NHEKs).
Time-lapse images of morphological changes in granular cells during cornification.
Z-stack images of nuclei in granular cells before and after flattening.
Images of the epidermal model Acidic vesicles in granular cells labelled with Hoechst 33258, CTG, and LTR.
Time-lapse images of acidic vesicles and morphological change in granular cells during cornification.
Images of granular cells at the start of observation in mitochondrial condition during cornification.
Images of granular cells 4hours after observation in mitochondrial condition during cornification.
time-lapse images of granular cells labelled with Hoechst 33258, CYTO-ID, and TMRE.
time-lapse images of granular cells labelled with Hoechst 33258, CYTO-ID, and MTR.
time-lapse images of granular cells labelled with CTB, MBG, and LTR.
Time-lapse images of the FLG-KD model in the granular cell labelled with Hoechst 33258, CTG, and LTR.
Time-lapse images of the control model in the granular cell labelled with Hoechst 33258, CTG, and LTR.
Images of keratinocytes labelled with MBG 30 min after treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.
Images of keratinocytes labelled with MBG 5 min after treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.
Images of keratinocytes labelled with MBG before treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.
Images of keratinocytes labelled with MTR 30 min after treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.
Images of keratinocytes labelled with MTR 5 min after treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.
Images of keratinocytes labelled with MTR before treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.
Images of keratinocytes labelled with TMRE 30 min after treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.
Images of keratinocytes labelled with TMRE 5 min after treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.
Images of keratinocytes labelled with TMRE before treatment with a mixture of antimycin A (10 μM) and oligomycin complex (2 μg/mL) to induce mitochondrial depolarization.