177-Kinjo-cAMPsignalingDyn
Author reviewedFluorescence images of the cAMP-Signaling Pathway in 3D Tissues with FRET-Assisted Two-Photon Activation of bPAC.
Project ID
177-Kinjo-cAMPsignalingDyn
Title
Fluorescence images of the cAMP-Signaling Pathway in 3D Tissues with FRET-Assisted Two-Photon Activation of bPAC.
Description
-
Release Date
2022-03-31
Update Date
-
License
Version
1.0.0
Method Summary
See details in Kinjo T, et. al. (2020) ACS Chem Biol., 15(11):2848-2853.
Kind
Image data
Organism
Homo sapiens
(
NCBITaxon:9606
)
Canis lupus familiaris
(
NCBITaxon:9615
)
Strain
-
Cell line
HeLa cell
(
CLO:0003684
)
MDCK cell
(
CLO:0007646
)
Biological Process
cAMP-mediated signaling
(
GO:0019933
)
Cellular Component
cAMP-dependent protein kinase complex
(
GO:0005952
)
Medical Subject Headings
Adenylyl Cyclases
(
MeSH:D000262
)
Adenylyl Cyclases/metabolism
(
MeSH:Q000378
)
Animals
(
MeSH:D000818
)
Bacteria
(
MeSH:D001419
)
Bacteria/enzymology
(
MeSH:Q000201
)
Biosensing Techniques
(
MeSH:D015374
)
Biosensing Techniques/methods
(
MeSH:Q000379
)
Cell Communication
(
MeSH:D002450
)
Cyclic AMP
(
MeSH:D000242
)
Cyclic AMP/metabolism
(
MeSH:Q000378
)
Cyclic AMP-Dependent Protein Kinases
(
MeSH:D017868
)
Cyclic AMP-Dependent Protein Kinases/metabolism
(
MeSH:Q000378
)
Dogs
(
MeSH:D004285
)
Enzyme Activation
(
MeSH:D004789
)
Fluorescence Resonance Energy Transfer
(
MeSH:D031541
)
Fluorescence Resonance Energy Transfer/methods
(
MeSH:Q000379
)
Fluorescent Dyes
(
MeSH:D005456
)
Fluorescent Dyes/metabolism
(
MeSH:Q000378
)
Green Fluorescent Proteins
(
MeSH:D049452
)
Green Fluorescent Proteins/metabolism
(
MeSH:Q000378
)
HeLa Cells
(
MeSH:D006367
)
Humans
(
MeSH:D006801
)
Liver
(
MeSH:D008099
)
Liver/metabolism
(
MeSH:Q000378
)
Madin Darby Canine Kidney Cells
(
MeSH:D061985
)
Mice
(
MeSH:D051379
)
Signal Transduction
(
MeSH:D015398
)
Single-Cell Analysis
(
MeSH:D059010
)
Single-Cell Analysis/methods
(
MeSH:Q000379
)
Method involved in biological imaging
differences in fluorescence lifetime
(
FBbi:00000605
)
FRET
(
FBbi:00000367
)
time lapse microscopy
(
FBbi:00000249
)
Image datasets
112
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Kenta Terai
Graduate School of Medicine, Kyoto University
Department of Pathology and Biology of Diseases
-
Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8315 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Fluorescence lifetime images of Hela cells expressing 2pabPAC.
Fluorescence lifetime images of Hela cells expressing mTFP1 alone.
Fluorescence images of HeLa cells expressing Booster-PKA coexpressed with 2pabPAC(Y72L)-P2A-mTFP1 and irradiated with 1P.
Fluorescence images of HeLa cells expressing Booster-PKA coexpressed with 2pabPAC(Y72L)-P2A-mTFP1 and irradiated with 2P.
Fluorescence images of HeLa cells expressing Booster-PKA coexpressed with 2pabPAC(Y72L)-P2A-mTFP1 before irradiated with 1P or 2P.
Fluorescence images of HeLa cells expressing Booster-PKA coexpressed with 2pabPAC-P2A-mTFP1 and irradiated with 1P.
Fluorescence images of HeLa cells expressing Booster-PKA coexpressed with 2pabPAC-P2A-mTFP1 and irradiated with 2P.
Fluorescence images of HeLa cells expressing Booster-PKA coexpressed with 2pabPAC-P2A-mTFP1 before irradiated with 1P or 2P.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cyst after single-cell 2pabPAC activation by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cyst after single-cell 2pabPAC activation by 2P excitation and whole-cell activation by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cyst under dark conditions before excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts with 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC before activated by 2P or 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 2P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC activated by 1P excitation.
Canis lupus familiaris
(
NCBITaxon:9615
)
Timelapse images of the MDCK cysts without 2pabPAC before activated by 2P or 1P excitation.