120-Ding-CentromericDyn thumbnail

120-Ding-CentromericDyn

Author reviewed
Timelapse images and BDML file for quantitative information about the centromeric dynamics during mouse oocytes meiosis
Project ID
120-Ding-CentromericDyn
Title
Timelapse images and BDML file for quantitative information about the centromeric dynamics during mouse oocytes meiosis
Description
-
Release Date
2021-09-30
Update Date
-
License
Version
1.0.0
Method Summary
See details in Ding Y, et. al. (2018) Current Biology, 28(10): 1661-1669.
Paper DOI
Paper URL
Kind
Image data
Total
67983 files / 1.2 TB
Image datasets
zipped 351 files / 506.3 GB, raw image 67632 files / 720.8 GB, total 67983 files / 1.2 TB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism
Mus musculus ( NCBITaxon:10090 )
Strain
-
Cell line
-
Biological Process
centromeric sister chromatid cohesion ( GO:0070601 )
meiotic cell cycle ( GO:0051321 )
Cellular Component
kinetochore ( GO:0000776 )
chromosome ( GO:0005694 )
Medical Subject Headings
Animals ( MeSH:D000818 )
Centromere ( MeSH:D002503 )
Centromere/physiology ( MeSH:Q000502 )
Female ( MeSH:D005260 )
Meiosis ( MeSH:D008540 )
Meiosis/physiology ( MeSH:Q000502 )
Mice ( MeSH:D051379 )
Oocytes ( MeSH:D009865 )
Oocytes/physiology ( MeSH:Q000502 )
Signal Transduction ( MeSH:D015398 )
Small Ubiquitin-Related Modifier Proteins ( MeSH:D025841 )
Small Ubiquitin-Related Modifier Proteins/physiology ( MeSH:Q000502 )
Method involved in biological imaging
time lapse microscopy ( FBbi:00000249 )
fluorescence microscopy ( FBbi:00000246 )

Image datasets
351
Quantitative datasets
0

Paper DOI / Paper URL

OMERO.gallery
OMERO
downloads

People
Contact
Tomoya S. Kitajima
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Chromosome Segregation
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-

Datasets

Thumbnail
Dataset
Organism
Kind / Links
Timelapse confocal images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase II
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 12 hours
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 10 hours
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 4 hours
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 8 hours
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 9 hours
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase II
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase II
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase II
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase I
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase I
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at telophase I
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at telophase I
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at telophase I
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at telophase I
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase I
Timelapse confocal images of mNeonGreen (mNG)-fused a small ubiquitin-related modifier(SUMO)2gg signals exhibit progressively enriched at centromeres during anaphase I and telophase I in mouse oocytes
Timelapse confocal images of mNeonGreen (mNG)-fused a small ubiquitin-related modifier(SUMO)2gg signals exhibit progressively enriched at centromeres during anaphase I and telophase I in mouse oocytes
Timelapse confocal images of mouse oocytes of constitutive kinetochore component CENP-C at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of wild-type PIAS1 fused to CENP-C (PIAS-CENP-C) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of a dominant-negative form of PIAS1 (C350S mutation in the SP-RING domain PIAS(dn)) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of constitutive kinetochore component CENP-C at metaphase I
Timelapse confocal images of mouse oocytes of wild-type PIAS1 fused to CENP-C (PIAS-CENP-C) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of a dominant-negative form of PIAS1 (C350S mutation in the SP-RING domain PIAS(dn)) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes at metaphase II (mNG-fused constructs:green, H2B-mCherry:red)
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during telophase I as control (SUMO2/3:green, kinetochores:ACA, red, DNA:blue)
Immunostaining images of mouse oocytes of wild-type PIAS1 fused to CENP-C during telophase I (SUMO2/3:green, kinetochores:ACA, red, DNA:blue)
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of wild-type PIAS1 fused to CENP-C during metaphase II (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of wild-type PIAS1 fused to CENP-C during metaphase II (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of wild-type PIAS1 fused to CENP-C during metaphase II (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )