89-Isshiki-SpineDyn thumbnail

89-Isshiki-SpineDyn

Author reviewed
Project name
89-Isshiki-SpineDyn
Release date
2018-11-14
Update date
-
Pre-release URL
-
Project title
Time-lapse images of spine dynamics in patDp/+ or NLG R451C and wild-type mice
License
Method summary
See details in Isshiki et al. (2014) Nat Commun, 5: 4742.
PubMed URL
Kind
Image data
Organism
Mus musculus ( NCBITaxon:10090 )
Total
81 files / 3.8 GB
Image datasets
zipped 41 files / 1.3 GB, raw image 40 files / 2.5 GB, total 81 files / 3.8 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Image datasets
40
Quantitative datasets
0

Strain
-
Datatype
spine dynamics
Basedon
Experiment
XYZ-scale
XY: 0.1534 micrometer/pixel, Z: 0.75 micrometer/slice
T-scale
2 day for each time interval
1 day for each time interval
dblink
None
External links
None

Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FV-300
Detector model
-
Objective model
XLPlan N, numerical aperture 1.05, Olympus
Filter set
-

Biological Process
-
Cellular Component
spine synapse ( GO:0106033 ) , postsynaptic density ( GO:0014069 )
People
Contact
Shigeo Okabe
University of Tokyo
Department of Cellular Neurobiology, Graduate School of Medicine
-
7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 Japan
Contributors
Masaaki Isshiki, Shinji Tanaka, Toshihiko Kuriu, Katsuhiko Tabuchi, Toru Takumi, Shigeo Okabe

OMERO.gallery
OMERO
downloads

Dataset List

Thumbnail
Dataset
Organism
Kind / Links
Time-lapse image of lost spines located near a shaft gephyrin cluster in wild-type mice at day 0.

Time-lapse image of lost spines located near a shaft gephyrin cluster in wild-type mice at day 2.

Time-lapse image of lost spines located away from a s shaft gephyrin cluster in patDp/+ mice at day 0.

Time-lapse image of lost spines located away from a s shaft gephyrin cluster in patDp/+ mice at day 2.

Time-lapse image of PSD-95 clusters in the SSC at 8 week of potnatal development.

Time-lapse image of PSD-95 clusters in the SSC at 14days of potnatal development.

Time-lapse image of PSD-95 clusters in the SSC at 21days of potnatal development.

Time-lapse image of PSD-95 clusters in the SSC at 7days of potnatal development.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 at 14days of potnatal development.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 at 15days of potnatal development.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 at day 0.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 over 2 days.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the SSC of patDp/+ mice at day 0.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the SSC of patDp/+ mice over 2 days.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice at day 0.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice over 2 days.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of patDp/+ mice at day 0.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of patDp/+ mice over 2 days.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice at day 0.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice over 2 days.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the AFC of patDp/+ mice at day 0.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the AFC of patDp/+ mice over 2 days.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the AFC of wild-type mice at day 0.

In vivo time-lapse image of PSD-95-GFP and dendries filled with DsRed2 in the AFC of wild-type mice over 2 days.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the SSC of patDp/+ mice at postnatal week 2.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the SSC of patDp/+ mice after 1 day of postnatal week 2.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice at postnatal week 2.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice after 1 day of postnatal week 2.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the AFC of NLGR51C mice at day 0.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the AFC of NLGR51C mice over 2 days.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the AFC of wild-type mice at day 0.

In vivo time-lapse image of PSD-95-GFP and dendrites filled with DsRed2 in the AFC of wild-type mice over 2 days.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of NLGR451C mice at day 0.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of NLGR451C mice over 2 days.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice at day 0.

In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice over 2 days.

Time-lapse image of gephyrin-GFP clusters on the dendritic shaft at day 0.

Time-lapse image of gephyrin-GFP clusters on the dendritic shaft over 2 days.

Time-lapse image of gephyrin-GFP clusters on the dendritic spines at day 0.

Time-lapse image of gephyrin-GFP clusters on the dendritic spines over 2 days.