87-CorneliaKoeverle-SpineDyn
Author reviewedProject name
87-CorneliaKoeverle-SpineDyn
Release date
2018-11-14
Update date
-
Pre-release URL
-
Project title
Images and time-lapse images of spine formation in wild type and CaMKIIaKI hippocampal neurons.
License
Method summary
See details in Koeberle et al. (2017) Sci Rep, 7(1): 13409.
PubMed URL
Kind
Image data
Organism
Mus musculus
(
NCBITaxon:10090
)
Total
79 files / 275.4 MB
Image datasets
zipped 31 files / 70.5 MB, raw image 48 files / 204.9 MB, total 79 files / 275.4 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
30
Quantitative datasets
0
Strain
-
Datatype
spine dynamics
Basedon
Experiment
XYZ-scale
XY: 0.072 micrometer/pixel, Z: 0.500 micrometer/slice
XY: 0.104 micrometer/pixel, Z: 0.700 micrometer/slice
XY: 0.155 micrometer/pixel, Z: 0.400 micrometer/slice
XY: 0.0932 micrometer/pixel, Z: 0.400 micrometer/slice
XY: 0.082 micrometer/pixel, Z: 0.500 micrometer/slice
T-scale
1 day for each time interval
1800 second for each time interval
15 second for each time interval
dblink
None
External links
None
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Biological Process
spine apparatus formation
(
GO:1905355
)
Cellular Component
spine synapse
(
GO:0106033
)
,
neuron spine
(
GO:0044309
)
People
Contact
Shigeo Okabe
University of Tokyo
Department of Cellular Neurobiology, Graduate School of Medicine
-
7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 Japan
Contributors
Solveigh Cornelia Koeberle, Shinji Tanaka, Toshihiko Kuriu, Hirohide Iwasaki, Andreas Koeberle, Alexander Schulz, Dario-Lucas Helbing, Yoko Yamagata, Helen Morrison, Shigeo Okabe
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Representative images of spine densities of apical dendrites at different developmental stages in CaMKIIa KI hippocampal neurons.
Representative images of spine densities of basal dendrites at different developmental stages in CaMKIIa KI hippocampal neurons.
Representative images of spine densities of oblique dendrites at different developmental stages in CaMKIIa KI hippocampal neurons.
Representative images of spine densities of apical dendrites at different developmental stages in wild type neurons.
Representative images of spine densities of basal dendrites at different developmental stages in wild type neurons.
Representative images of spine densities of oblique dendrites at different developmental stages in wild type neurons.
Time-lapse image of spine formation and elimination in CA1 pyramidal neurons in CaMKIIa KI hippocampal slices at day1.
Time-lapse image of spine formation and elimination in CA1 pyramidal neurons in CaMKIIa KI hippocampal slices at day2.
Time-lapse image of spine formation and elimination in CA1 pyramidal neurons in wild-type hippocampal slices at day1.
Time-lapse image of spine formation and elimination in CA1 pyramidal neurons in wild-type hippocampal slices at day2.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP and treated with the CaMKII inhibitor KN92 at day19.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP and treated with the CaMKII inhibitor KN92 at day19.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP and treated with the CaMKII inhibitor KN92 at day20.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP and treated with the CaMKII inhibitor KN93 at day19.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP and treated with the CaMKII inhibitor KN93 at day19.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP and treated with the CaMKII inhibitor KN93 at day20.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP along with GFP-tagged wild-type CaMKII at day14.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP along with GFP-tagged wild-type CaMKII at day14.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP along with GFP-tagged wild-type CaMKII at day16.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP along with kinase-dead mutant of CaMKII(K42R) at day14.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP along with kinase-dead mutant of CaMKII(K42R) at day14.
Time-lapse image of spines in dissociated hippocampal neurons expressing RFP along with kinase-dead mutant of CaMKII(K42R) at day16.
Time-lapse FRET image of Rap1 activity in dissociated hippocampal neurons treated with the CaMKII inhibitor .
Time-lapse FRET image of Rap1 activity in dissociated hippocampal neurons treated with the CaMKII inhibitor .
Time-lapse FRET image of Rap1 activity in dissociated hippocampal neurons treated with the CaMKII inhibitor .
Time-lapse FRET image of Rap1 activity in dissociated hippocampal neurons treated with the CaMKII inhibitor inactive analogue.
Time-lapse FRET image of Rap1 activity in dissociated hippocampal neurons treated with the CaMKII inhibitor inactive analogue.
Time-lapse FRET image of Rap1 activity in dissociated hippocampal neurons treated with the CaMKII inhibitor inactive analogue.
Time-lapse image of DsRed2 and PAGFP-actin in the single spines of KI neurons.
Time-lapse image of DsRed2 and PAGFP-actin in the single spines of wild-type.