86-Higashi-SynapseDyn
Author reviewedProject name
86-Higashi-SynapseDyn
Release date
2019-11-20
Update date
-
Pre-release URL
-
Project title
Images and time-lapse images of BMP4-dependent synapse dynamics in drosophila
License
Method summary
See details in Higashi et al. (2018) Cell Rep, 22(4): 919-929.
PubMed URL
Kind
Image data
Organism
Mus musculus
(
NCBITaxon:10090
)
Total
32 files / 2.0 GB
Image datasets
zipped 16 files / 549.0 MB, raw image 16 files / 1.4 GB, total 32 files / 2.0 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
16
Quantitative datasets
0
Strain
-
Datatype
synapse dynamics
Basedon
Experiment
XYZ-scale
XY: XX micrometer/pixel, Z: XX micrometer/slice
XY: 0.225micrometer/pixel, Z: NA
XY: 0.225 micrometer/pixel, Z: NA
XY: 0.207 micrometer/pixel , Z: NA
XY: 0.132 micrometer/pixel, Z: NA
XY: 0.103 micrometer/pixel, Z:1 micrometer/slice
XY: 0.1534 micrometer/pixel, Z: 0.75 micrometer/slice
T-scale
0.5 second for each time interval
1 minute for each time interval
30 minute for each time interval
dblink
None
External links
None
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FluoView FV10i
Detector model
-
Objective model
-
Filter set
-
Biological Process
synaptogenesis
(
GO:0007416
)
,
neuromuscular junction
(
GO:0031594
)
Cellular Component
spine synapse
(
GO:0106033
)
People
Contact
Shigeo Okabe
University of Tokyo
Department of Cellular Neurobiology, Graduate School of Medicine
-
7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 Japan
Contributors
Takahito Higashi, Shinji Tanaka, Tadatsune Iida, Shigeo Okabe
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Images of BMP4-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 0 minute
Images of BMP4-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 0 minute
Images of BMP4-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 180 minute
Images of BMP4-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 180 minute
Images of BSA-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 0 minute
Images of BSA-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 0 minute
Images of BSA-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 180 minute
Images of BSA-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 180 minute
Time-lapse images of BMP4-GEFP and NPY-mCherry in the axon of cultured hippocampal neurons.
Time-lapse images of fluorescently labeled BMP4 together with synaptophysin-tagRFP in neurons at DIV14.
Time-lapse images of fluorescently labeled BMP4 together with PSD-95-EGFP in neurons at DIV14.
Time-lapse images of synaptophysin-tagRFP cluster associated with BMP4-SEP.
Time-lapse images of synaptophysin-EGFP clusters at day 0 in the axon of Cre-expressing pyramidal neurons (BMP4 flox/flox knock out genotype)
Time-lapse images of synaptophysin-EGFP clusters at day 2 in the axon of Cre-expressing pyramidal neurons (BMP4 flox/flox knock out genotype)
Time-lapse images of synaptophysin-EGFP clusters at day 0 in control neurons
Time-lapse images of synaptophysin-EGFP clusters at day 2 in control neurons