84-Ito-CellDyn
Author reviewedProject name
84-Ito-CellDyn
Release date
2018-11-14
Update date
-
Pre-release URL
-
Project title
Timelapse images of different types HT29 cells during nocodazole treatment
License
Method summary
See details in Ito et al. (2017) Nat Commun, 8(1): 1834-1849.
PubMed URL
Kind
Image data
Organism
Homo sapiens
(
NCBITaxon:9606
)
Total
15 files / 871.6 MB
Image datasets
zipped 8 files / 233.7 MB, raw image 7 files / 638.0 MB, total 15 files / 871.6 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
7
Quantitative datasets
0
Strain
HT29
Datatype
cell dynamics
Basedon
Experiment
XYZ-scale
XY: 353 micrometer/pixel, Z: NA micrometer/slice
T-scale
60 second for each time interval
120 second for each time interval
300 second for each time interval
dblink
None
External links
None
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
DIC
Microscope model
Olympus LCV100 or Olympus IX81 + Yokogawa CSU-W1
Detector model
Olympus DP30 or ImagEM-IK
Objective model
Olympus UAPOx40/340 or PLANAPO N x60/1.42 oil lens
Filter set
interference filter
Biological Process
-
Cellular Component
apical junction complex
(
GO:0043296
)
People
Contact
Masatoshi Takeichi
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Cell Adhesion and Tissue Patterning
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Contributors
Shoko Ito, Satoru Okuda, Masako Abe, Mari Fujimoto, Tetsuo Onuki, Tamako Nishimura, Masatoshi Takeichi
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of HT29 cells treated with 20 microMole nocodazole
Time-lapse images of MLC2-EGFP expressed in HT29 cells during nocodazole treatment
Time-lapse images of Lifeact-EGFP expressed in HT29 cells during nocodazole treatment in control
Time-lapse images of Lifeact-EGFP expressed in HT29 cells during nocodazole treatment in αE-catenin depleted cells
Time-lapse images of HT29 cells treated with nocodazole
Time-lapse images of HT29 cells treated with nocodazole
Time-lapse images of a RhoA-specific Raichu probe expressed HT29 cells