83-Fumoto-LungDevWnt
Author reviewedProject name
83-Fumoto-LungDevWnt
Release date
2018-11-14
Update date
-
Pre-release URL
-
Project title
Modulation of apical constriction by Wnt signaling is required for lung epithelial shape transition
License
Method summary
See details in Fumoto et al. (2017) Development, 144(1): 151-162.
PubMed URL
Kind
Image data
Organism
Mus musculus
(
NCBITaxon:10090
)
Total
86 files / 61.7 MB
Image datasets
zipped 19 files / 30.7 MB, raw image 67 files / 31.0 MB, total 86 files / 61.7 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
18
Quantitative datasets
0
Strain
-
Datatype
organ formation
Basedon
Experiment
XYZ-scale
XY: 0.2075 micrometer/pixel, Z: NA
XY: 0.415 micrometer/pixel, Z: NA
XY: NA, Z: NA
T-scale
24 hour for each time interval
1 day for each time interval
dblink
None
External links
None
Experiment type
Immunofluorescence
Microscope type
FluorescenceMicroscope
Acquisition mode
Other
Contrast method
Fluorescence
Microscope model
Carl Zeiss, LSM880
Detector model
-
Objective model
-
Filter set
-
Biological Process
lung development
(
G0:0030324
)
Cellular Component
cytoskeleton
(
GO:005856
)
People
Contact
Akira Kikuchi
Departments of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita 565-0871, Japan.
Graduate School of Medicine
Departments of Molecular Biology and Biochemistry
2-2, Yamadaoka, Suita 565-0871, Japan
Contributors
Katsumi Fumoto, Hisako Takigawa-Imamura, Kenta Sumiyama, Tomoyuki Kaneiwa, Akira Kikuchi
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Immunostaining image of embryonic lungs at E14 with anti-E-cadherin(green)and anti-ppMLC(red) antibodies, and phalloiding(gray)
Immunostaining image of embryonic lungs at E17 with anti-E-cadherin(green)and anti-ppMLC(red) antibodies, and phalloiding(gray)
Immunostaining image of embryonic lungs at E14 with anti-E-cadherin(green)and anti-a-catenin(red) and a18(gray) antibodies
Immunostaining image of embryonic lungs at E17 with anti-E-cadherin(green)and anti-a-catenin(red) and a18(gray) antibodies
Immunostaining image of embryonic lungs at E14 with anti-Pdpn(green)and anti-E-cadherin(red) antibodies and phalloidin(blue)
Immunostaining image of embryonic lungs at E17 with anti-Pdpn(green)and anti-E-cadherin(red) antibodies and phalloidin(blue)
Image of whole lung rudiments treated with 5uMXAV939 cultured for 0 hour,24 hours, 48 hours and 72 hours
Image of whole lung rudiments treated without 5uMXAV939 cultured for 0 hour,24 hours, 48 hours and 72 hours
Image of mesenchyme-free lung epithelium was embedded in Matrigel and cultured in serum-free medium 200ng/ml FGF10 in the presence of 1uMCHIR99021 for 5 days
Image of mesenchyme-free lung epithelium was embedded in Matrigel and cultured in serum-free medium 200ng/ml FGF10 in the absence of 1uMCHIR99021 for 5 days
Image of mesenchyme-free lung epithelium was embedded in Matrigel and cultured in serum-free medium 500ng/ml FGF10 for 5 days
Image of embryonic lungs at E14 were stained with anti-Mark1 (red) and anti-E-cadherin (green) antibodies
Image of embryonic lungs at E17 were stained with anti-Mark1 (red) and anti-E-cadherin (green) antibodies
Image of Mesenchyme-free lung epithelium infected with shMark1_1 was cultured for 5 days in FGF10-CHIR
Image of Mesenchyme-free lung epithelium infected with shMark1_1 was cultured for 5 days in FGF10-CHIR
Image of Mesenchyme-free P1 lung epithelium cultured with low FGF10 and without CHIR99021 for 48 hours
Image of Mesenchyme-free P1 lung epithelium cultured with low FGF10 and 1 μM CHIR99021 for 48 hours
Image of Mesenchyme-free P1 lung epithelium cultured with low FGF10 and 2.5 μM CHIR99021 for 48 hours