79-Inomata-HeLaCell thumbnail

79-Inomata-HeLaCell

Author reviewed
Project name
79-Inomata-HeLaCell
Release date
2018-11-14
Update date
-
Pre-release URL
-
Project title
Intracellular distribution of hAK1 and hUB-3A delivered into HeLa cells.
License
Method summary
See details in Inomata et al. (2017) Chem Commun, 53(81): 11245-11248.
PubMed URL
Kind
Image data
Organism
Homo sapiens ( NCBITaxon:9606 )
Total
17 files / 7.9 MB
Image datasets
zipped 9 files / 1.9 MB, raw image 8 files / 6.0 MB, total 17 files / 7.9 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Image datasets
8
Quantitative datasets
0

Strain
HeLa
Datatype
intracellular structure
Basedon
Experiment
XYZ-scale
XY: 0.17297 micrometer/pixel, Z: NA
T-scale
-
dblink
None
External links
None

Experiment type
Other
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FluoView FV10i-DOC
Detector model
-
Objective model
-
Filter set
-

Biological Process
cellular localization ( GO:1902580 )
Cellular Component
intracellular organelle ( GO:0043229 )
People
Contact
Kohsuke Inomata, Takanori Kigawa
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Cellular Structural Biology
1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
Contributors
Kohsuke Inomata, Hajime Kamoshida, Masaomi Ikari, Yutaka Ito, Takanori Kigawa

OMERO.gallery
OMERO
downloads

Dataset List

Thumbnail
Dataset
Organism
Kind / Links
Homo sapiens ( NCBITaxon:9606 )
Fluorescence image of nuclear stainging with Hoechst 33342 in HeLa cells.

Homo sapiens ( NCBITaxon:9606 )
Fluorescence image of intracellular distribution of Alexa488-labeled hAK1 in HeLa cells.

Homo sapiens ( NCBITaxon:9606 )
Fluorescence image of nuclear stainging with Hoechst 33342 and intracellular distribution of Alexa488-labeled hAK1 in HeLa cells.

Homo sapiens ( NCBITaxon:9606 )
Phase contrast image of living HeLa cells containing the delivered hAK1 at 2h after electroporation.

Homo sapiens ( NCBITaxon:9606 )
Fluorescence image of nuclear stainging with Hoechst 33342 in HeLa cells.

Homo sapiens ( NCBITaxon:9606 )
Fluorescence image of intracellular distribution of Alexa488-labeled hUb-3A in HeLa cells.

Homo sapiens ( NCBITaxon:9606 )
Fluorescence image of nuclear stainging with Hoechst 33342 and intracellular distribution of Alexa488-labeled hUb-3A in HeLa cells.

Homo sapiens ( NCBITaxon:9606 )
Phase contrast image of living HeLa cells containing the delivered hUb-3A at 4h after electroporation.