SSBD:database

72-Kyogoku-CytoplasmNucDyn

Author reviewed

SSBD:OMERO.gallery SSBD:OMERO

SSBD:downloads

Project name

72-Kyogoku-CytoplasmNucDyn

Release date

2021-11-30

Update date

Pre-release URL

Project title

Timelapse images and BDML file for quantitative information about the cytoplasmic size affecting nuclear dynamics during mouse oocytes meiosis

License

CC BY

Method summary

See details in Kyogoku et al. (2017) Dev. Cell, 41(3): 287-298.

PubMed URL

https://www.ncbi.nlm.nih.gov/pubmed/28486131

Kind

Image data

Organism

Mus musculus ( NCBITaxon:10090 )

Total

724063 files / 10.3 TB

Image datasets

zipped 453 files / 3.5 TB, raw image 723610 files / 6.7 TB, total 724063 files / 10.3 TB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Image datasets

Quantitative datasets

Strain

Datatype

nuclear dynamics

Basedon

Experiment

XYZ-scale

XY: 0.1037832 micrometer/pixel, Z: 1 micrometer/slice

XY: 0.1037832 micrometer/pixel, Z: 4 micrometer/slice

XY: 0.0922518 micrometer/pixel, Z: 4 micrometer/slice

XY: 0.0922518 micrometer/pixel, Z: 1 micrometer/slice

XY: 0.4151329 micrometer/pixel, Z: 4 micrometer/slice

XY: 0.1037832 micrometer/pixel, Z: 1.5 micrometer/slice

XY: NA, Z: NA

T-scale

5 minutes for each time interval

dblink

None

External links

None

Experiment type

Microscope type

ConfocalMicroscope

Acquisition mode

LaserScanningConfocalMicroscopy

Contrast method

Fluorescence

Microscope model

Detector model

Objective model

40x C-Apochromat 1.2NA water immersion

Filter set

Biological Process

nuclear envelope breakdown ( GO:0051081 ) , mitotic cell cycle ( GO:0007067 ) , cytoplasm organization ( GO:0007028 )

Cellular Component

kinetochore microtubule ( GO:0005828 ) , chromosome ( GO:0005694 )

People

Contact

Tomoya S. Kitajima

RIKEN

Center for Biosystems Dynamics Research

Laboratory for Chromosome Segregation

2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan

Contributors

Hirohisa Kyogoku, Tomoya S. Kitajima

OMERO.gallery

https://ssbd.riken.jp/omero/gallery/show_project/662/

OMERO

https://ssbd.riken.jp/omero/webclient/?show=project-662

downloads

https://ssbd.riken.jp/data/72-Kyogoku-CytoplasmNucDyn/

Dataset List

Thumbnail

Dataset

Organism

Kind / Links

Figure1B_Doubled_cytoplasmic

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Timelapse confocal image of doubled cytoplasmic mouse oocytes during meiosis I

Figure1B_Doubled_cytoplasmic_EGFP

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Timelapse confocal image of EGFP-expressing doubled cytoplasmic mouse oocytes during meiosis I

Figure1B_Half_cytoplasmic

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Timelapse confocal image of halved cytoplasmic mouse oocytes during meiosis I

Figure1B_Half_cytoplasmic_EGFP

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Timelapse confocal image of EGFP-expressing halved cytoplasmic mouse oocytes during meiosis I

Figure1B_control

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Timelapse confocal image of mouse oocytes during meiosis I as control

Figure1B_control_EGFP

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Timelapse confocal image of EGFP-expressing mouse oocytes during meiosis I as control

Figure1C_2013_12_19

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes during meiosis I

Figure1C_2014_1_31

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes during meiosis I

Figure1C_2014_3_19

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes during meiosis I

Figure1C_2014_4_2

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes during meiosis I

Figure2B_2015_8_10

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of halved cytoplasmic mouse oocytes during meiosis I

Figure2B_2015_8_13

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of halved cytoplasmic mouse oocytes during meiosis I

Figure2B_2015_8_6

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of halved cytoplasmic mouse oocytes during meiosis I

Figure2B_Halved_After_NEBD

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes halved after nuclear envelope breakdown (NEBD)

Figure2B_Halved_Before_NEBD

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes halved before nuclear envelope breakdown (NEBD)

Figure2C_2015_11_20_after

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes fused after iamging

Figure2C_2015_11_20_before

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes fused before imageing

Figure2D_2015_11_17

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of fused mouse oocytes during meiosis I

Figure2D_2015_11_18

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of fused mouse oocytes during meiosis I

Figure2D_2015_11_18_after

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes fused after iamging

Figure2D_2015_11_18_before

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes fused before imageing

Figure2D_2015_11_20

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of fused mouse oocytes during meiosis I

Figure2D_2015_12_9

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of fused mouse oocytes during meiosis I

Figure2D_2015_12_9_after

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes fused after iamging

Figure2D_2015_12_9_before

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mouse oocytes fused before imageing

Figure2D_2016_2_10

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of fused mouse oocytes during meiosis I

Figure2D_2016_2_17

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of fused mouse oocytes during meiosis I

Figure3A_EFcontrol

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mNeonGreen-Cep 192 (green) and H2B-mCherry (red) expressing mouse oocytes

Figure3A_Half

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mNeonGreen-Cep 192 (green) and H2B-mCherry (red) expressing halved cytoplasmic mouse oocytes

Figure3A_doubled

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of mNeonGreen-Cep 192 (green) and H2B-mCherry (red) expressing doubled cytoplasmic mouse oocytes

Figure3BC_2015_12_10_2

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation during mouse ooctytes meiosis I

Figure3BC_2015_12_16

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation during mouse ooctytes meiosis I

Figure3BC_2015_12_18

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation during mouse ooctytes meiosis I

Figure3BC_2016_1_4

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation during mouse ooctytes meiosis I

Figure3BC_2016_1_5

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation during mouse ooctytes meiosis I

Figure3BC_2016_1_6

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation during mouse ooctytes meiosis I

Figure3BC_Double_20151220_L10_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L11_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L12_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L13_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L14_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L1_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L3_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L4_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L5_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L6_MTOC

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure3BC_Double_20151220_L7

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of microtubule organized centre (MTOC) deformation of doubled cytoplasmic mouse ooctytes at meiosis I

Figure5D_KTtracking_20160223_L19_con1

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of tracking intra-polar chromosome stretching in normal mouse oocytes expressing 2mNeonGreen-CENP-C (kinetochores, green) and H2B- mCherry (chromosomes, red)

Figure5D_KTtracking_20160223_L19_con2

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of tracking intra-polar chromosome stretching in normal mouse oocytes expressing 2mNeonGreen-CENP-C (kinetochores, green) and H2B- mCherry (chromosomes, red)

Figure5D_KTtracking_20160223_L19_con3

Mus musculus ( NCBITaxon:10090 )

Image data omero.gallery omero

Live cell imaging of tracking intra-polar chromosome stretching in normal mouse oocytes expressing 2mNeonGreen-CENP-C (kinetochores, green) and H2B- mCherry (chromosomes, red)