70-Yoshida-KTMTDyn
Author reviewedProject name
70-Yoshida-KTMTDyn
Release date
2021-09-30
Update date
-
Pre-release URL
-
Project title
Timelapse confocal images and BDML files for quantitative information about the kinetochore microtubule dynamics during mouse oocytes meiosis
License
Method summary
See details in Yoshida et al. (2015) Dev. Cell, 33(5): 589-602.
PubMed URL
Kind
Image data
Organism
Mus musculus
(
NCBITaxon:10090
)
Total
73406 files / 611.9 GB
Image datasets
zipped 265 files / 240.4 GB, raw image 73141 files / 371.5 GB, total 73406 files / 611.9 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
50
Quantitative datasets
0
Strain
-
Datatype
kinetochore microtubule dynamics
Basedon
Experiment
XYZ-scale
XY: 0.0593047 micrometer/pixel, Z: 0.25 micrometer/slice
XY: 0.0593047 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.0263576 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.0593047 micrometer/pixel, Z: 1 micrometer/slice
XY: NA, Z: NA
T-scale
904 second for each time interval
903 second for each time interval
dblink
None
External links
None
Experiment type
-
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Zeiss LSM780
Detector model
-
Objective model
-
Filter set
-
Biological Process
mitotic cell cycle
(
GO:0007067
)
Cellular Component
kinetochore microtubule
(
GO:0005828
)
,
chromosome
(
GO:0005694
)
People
Contact
Tomoya S. Kitajima
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Chromosome Segregation
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Contributors
Shuhei Yoshida, Masako Kaido, Tomoya S. Kitajima
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 3 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 60 minutes (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 2 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 4 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytesduring MI for 5 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 2 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 4 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Immunostaining confocal image of representative kinetochore-microtubule (KT-MT) attachments of cold-treated mouse oocytes during MI for 5 hours (stained for MTs: green, KTs: red, chromosomes: bulue)
Timelapse confocal images of mEGFP-INCENP expressing oocytes at the end of the stretching phase (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of mEGFP-INCENP expressing oocytes during the stabilization phase (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of mEGFP-INCENP expressing oocytes at meiosis II (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of mEGFP-INCENP expressing oocytes at meiosis II (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of Hec1-mEGFP-CENP-C expressing oocytes during meiosis I for 2 hours (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of Hec1-mEGFP-CENP-C expressing oocytes during meiosis I for 4 hours (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of Hec1-mEGFP-CENP-C expressing oocytes during meiosis I for 6 hours (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Timelapse confocal images of Hec1-mEGFP-CENP-C expressing oocytes during meiosis II (stained for mEGFP: green, CENP-C: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of the metaphase plate of the meiosis I/MII fused mEGFP-hINCENP expressing oocytes (stained for INCENP: green, CENP-C: red, chromosomes: blue )
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of the metaphase plate of the meiosis I/MII fused mEGFP-Hec1 expressing oocytes (stained for INCENP: green, CENP-C: red, chromosomes: blue )
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MII for 2 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 4 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 6 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MII (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 2 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 4 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Immunostaining images of pS55-Hec1 expressing oocytes during meiosis MI for 6 hours (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of pS24-Knl1 expressing oocytes during meiosis MI for 2 hours (stained for pS24-knl1: green, KT: red, chromosomes: bulue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of pS24-knl1 expressing oocytes during meiosis MI for 4 hours (stained for pS24-knl1: green, KT: red, chromosomes: bulue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of pS24-knl1 expressing oocytes during meiosis MI for 6 hours (stained for pS24-knl1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of pS24-knl1 expressing oocytes during meiosis MII (stained for pS24-knl1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 1 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 2 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 3 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 4 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 5 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MI for 6 hour (stained for Hec1: green, KT: red, chromosomes: bulue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of Hec1 expressing oocytes during meiosis MII (stained for Hec1: green, KT: red, chromosomes: bulue)
Timelapse confocal images of pS55-Hec1 level of KTs in fused Hec1 expressing oocytes (stained for pS55-Hec1: green, KT: red, chromosomes: blue)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of oocytes epressing 3mEGFP-PP22A-B56e (green) and H2B-mCherry (red) cultured in control medium
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of oocytes epressing 3mEGFP-PP22A-B56e (green) and H2B-mCherry (red) cultured in the treatment with the Eg5 inhibitor monastrol (right)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of oocytes epressing 3mEGFP-PP22A-B56e (green) and H2B-mCherry (red) cultured in the control (left) or treatment with the Eg5 inhibitor monastrol (right)
Mus musculus
(
NCBITaxon:10090
)
Timelapse confocal images of oocytes epressing 3mEGFP-PP22A-B56e (green) and H2B-mCherry (red) cultured in the treatment with the Eg5 inhibitor monastrol (right)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 120 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 180 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 240 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 300 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 360 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e cultured in control medium for 60 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e culutured in the treatment with the Eg5 inhibitor monastrol for 120 minutes (choromosome: blue)
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of oocytes epressing 3mEGFP-PP22A-B56e culutured in the treatment with the Eg5 inhibitor monastrol for 240 minutes (choromosome: blue)