57-Herawati-CellDyn
Author reviewedProject name
57-Herawati-CellDyn
Release date
2018-11-14
Update date
2020-02-03
Pre-release URL
-
Project title
Images and quantitative data showing the self-organizing mechanism in mouse multiciliated cells (MCCs)
License
Method summary
See details in Herawati et al. (2016) J Cell Biol, 214(5): 571-586.
Kind
Quantitative data
Image data
Organism
Mus musculus
(
NCBITaxon:10090
)
Total
78 files / 6.5 GB
Image datasets
zipped 48 files / 4.1 GB, raw image 24 files / 2.4 GB, total 72 files / 6.5 GB
Quantitative datasets
zipped 2 files / 44.8 KB, raw bdml 4 files / 971.5 KB, total 6 files / 1016.3 KB
Image datasets
23
Quantitative datasets
1
Paper(s)
Strain
-
Datatype
epithelial cells dynamics
Basedon
Experiment
XYZ-scale
XY: NA, Z: NA
XY: 0.07 micrometer/pixel, Z: 0.07 micrometer/slice
T-scale
-
dblink
None
External links
None
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
SpinningDiskConfocal
Contrast method
Fluorescence
Microscope model
Olympus SD-OSR
Detector model
ORCA-Flash 4.0 v2; Hamamatsu Photonics
Objective model
Olympus UPlanSApo 60x Sil, ,NA 1.3; 1.6x conversion
Filter set
DAPI/FITC/TRITC
Biological Process
-
Cellular Component
cytoskeleton
(
GO:0005856
)
People
Contact
Sachiko Tsukita, Shuji Ishihara
Osaka University
Graduate School of Frontier Biosciences and Medicine
Laboratory of Biological Science
Contributors
Elisa Herawati, Daisuke Taniguchi, Hatsuho Kanoh, Kazuhiro Tateishi, Shuji Ishihara, Sachiko Tsukita
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
BDML file for quantitative information about tracing individual basal bodies (BBs) eight kinds of alignments indicated by different colors
Live image of showing cilia of GFP-centrin2 MTECs beating.
Live image of showing cilia of GFP-centrin2 MTECs beating.
Live image of showing cilia of GFP-centrin2 MTECs beating under normal incubation.
Time-lapse image of BBs forming alignment pattern after docking to the apical cell membrane.
Time-lapse image of BBs forming alignment pattern after docking to the apical cell membrane.
Time-lapse image of BBs forming alignment pattern after docking to the apical cell membrane.
Live image of tracking the dynamic behavior of individual BBs in MTEC multiciliated cells.
Time-lapse image of BBs in Odf2 mutant (Odf2ΔEx6,7ΔEx6,7) GFP-centrin2-transgenic MTECs.
Time-lapse image of GFP-centrin2–labeled BBs in MTEC multiciliated cells under nocodazole treatment in the early stage.
Time-lapse image of GFP-centrin2–labeled BBs in MTEC multiciliated cells under nocodazole treatment in the late stage.
Time-lapse image of GFP-centrin2–labeled BBs in MTEC multiciliated cells under nocodazole treatment in the middle stage.
Live image of GFP-centrin2 ZO-1 MTECS at air-liquid interface (ALI) 21 days.
Live image of GFP-centrin2 transgenic MTECS
Live image of interplaying between BB alignment and orientation.
Live image of interplaying between BB alignment and orientation.
Live image of interplaying between BB alignment and orientation.
Mus musculus
(
NCBITaxon:10090
)
Live image of interplaying between BB alignment and orientation.
Immunostained images of BB patern in GFP-centrin2-expressing MCCs with nocodazole treatment.
Mus musculus
(
NCBITaxon:10090
)
Immunostained images of BB patern in GFP-centrin2-expressing MCCs.
Immunostained images of an anti-keratin 8 mAb expression in Odf2-based BBs after nocodazole treatment.
Immunostained images of an anti-keratin 8 mAb expression in Odf2-based BBs.
Image of BBs in the Odf2 mutant (Odf2ΔEx6,7ΔEx6,7) GFP-centrin2-transgenic MTECs.
Image of BBs in the wild-type GFP-centrin2-transgenic MTECs.