34-Hayashi-CellDynSDSRM
Author reviewedProject name
34-Hayashi-CellDynSDSRM
Release date
2017-10-03
Update date
2018-11-15
Pre-release URL
-
Project title
-
License
Method summary
See details in Hayashi and Okada (2015) Mol Biol Cell, 26(9): 1743-1751.
PubMed URL
Kind
Image data
Organism
Canis lupus familiaris
(
NCBITaxon:9615
)
Total
6 files / 181.5 MB
Image datasets
zipped 3 files / 77.4 MB, raw image 3 files / 104.0 MB, total 6 files / 181.5 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
3
Quantitative datasets
0
Strain
MDCK
Datatype
organelle dynamics
Basedon
Experiment
XYZ-scale
XY: 100 nanometer per slice, Z: NA
T-scale
0.1 second for each time interval
0.01 second for each time interval
dblink
None
External links
None
Experiment type
TimeLapse
Microscope type
Other
Acquisition mode
StructuredIllumination
Contrast method
Fluorescence
Microscope model
Custom made microscope system based on IX81 (Olympus) with a disk-scannning unit IX2-DSU (Olympus), custom-made disk (DU-DSR-SP; Olympus) and laser illumination optics (Sapphire488HP; Coherent)
Detector model
ORCA FLASH 4.0 v2
Objective model
Olympus UPLSAPO60XO 60x/1.3 O
Filter set
Olympus NIBA filter set without excitation filter
Biological Process
-
Cellular Component
organelle
(
GO:0043226
)
People
Contact
Yasushi Okada
RIKEN
Quantitative Biology Center
Laboratory for Cell Polarity Regulation
Contributors
Shinichi Hayashi, Yasushi Okada
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse SDSRM images of rapidly moving lysosomes stained with Lamp1-EYFP in MDCK cells
Time-lapse SDSRM images of rapid dynamics of recycling endosomes stained with Rab11a-venus in MDCK cells
Time-lapse SDSRM images of the dynamics of the outer membrane of mitochondria stained with TOMM20-mEmelard in MDCK cells