21-Yasui-MolDynPTEN thumbnail

21-Yasui-MolDynPTEN

Author reviewed
Project name
21-Yasui-MolDynPTEN
Release date
2017-10-03
Update date
2018-11-15
Pre-release URL
-
Project title
-
License
Method summary
See details in Yasui et al. (2014) PLoS Computational Biology, 10(9): e1003817.
PubMed URL
Kind
Image data
Organism
Dictyostelium discoideum ( NCBITaxon:44689 )
Total
26 files / 1.7 GB
Image datasets
zipped 13 files / 569.4 MB, raw image 13 files / 1.2 GB, total 26 files / 1.7 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Image datasets
13
Quantitative datasets
0

Strain
-
Datatype
single molecule dynamics
Basedon
Experiment
XYZ-scale
XY: 0.033 micrometer/pixel, Z: NA
T-scale
33 millisecond for each time interval
dblink
None
External links
None

Experiment type
TimeLapse
Microscope type
TotalInternalReflectionFluorescenceMicroscope
Acquisition mode
TIRF
Contrast method
Fluorescence
Microscope model
OLYMPUS IX-70
Detector model
Andor iXon3
Objective model
OLYMPUS PlanApo 60x 1.45NA Oil TIRFM
Filter set
Dichroic: Omega Optical XF2032, Emission: Semrock FF01-575/25

Biological Process
chemotaxis ( GO:0006935 )
Cellular Component
-
People
Contact
Satomi Matsuoka
Osaka University
Graduate School of Frontier Biosciences
Laboratories for Nanobiology
1-3, Yamada, Suita, Osaka 565-0871, Japan
Contributors
Masato Yasui, Satomi Matsuoka, Masahiro Ueda

OMERO.gallery
OMERO
downloads

Dataset List

Thumbnail
Dataset
Organism
Kind / Links
Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of D. discoideum cells expressing wild-type PTEN

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of D. discoideum cells expressing PTEN mutants labeled with TMR

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of D. discoideum cells expressing PTEN mutants labeled with TMR

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of D. discoideum cells expressing PTEN mutants labeled with TMR

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of D. discoideum cells expressing PTEN mutants labeled with TMR

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of D. discoideum cells expressing PTEN mutants labeled with TMR

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of D. discoideum cells expressing PTEN mutants labeled with TMR

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of D. discoideum cells expressing PTEN mutants labeled with TMR

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of wild-type PTEN molecule hopping on D. discoideum cell membrane

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of wild-type PTEN molecule hopping on D. discoideum cell membrane

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of wild-type PTEN molecule hopping on D. discoideum cell membrane

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images of PTEN4 mutants molecule hopping on D. discoideum cell membrane

Dictyostelium discoideum ( NCBITaxon:44689 )
Time-lapse TIRF microscopy images showing a succession of wild-type PTEN molecule jumps on D. discoideum cells membrane