128-Muta-IECsERKDyn
Author reviewedProject name
128-Muta-IECsERKDyn
Release date
2021-09-30
Update date
-
Pre-release URL
-
Project title
Fluorescence images and BDML file for quantitative information about mouse intestinal epithelial cells (IECs) extracellular signal-regulated kinase (ERK) activity dynamics
License
Method summary
See details in Muta et al. (2018) Nat. Commun., 9(1): 2174.
PubMed URL
Kind
Image data
Organism
Mus muculus
(
NCBITaxon:10090
)
Total
181148 files / 94.5 GB
Image datasets
zipped 470 files / 32.0 GB, raw image 180678 files / 62.5 GB, total 181148 files / 94.5 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
50
Quantitative datasets
0
Strain
-
Datatype
intestinal epithelial cells ERK activity dynamics
Basedon
Experiment
XYZ-scale
XY: 0.828 micrometer/pixel, Z: 4 micrometer/slice
XY: 0.828 micrometer/pixel, Z: 2 micrometer/slice
XY: 0.497 micrometer/pixel, Z: 4 micrometer/slice
XY: 0.497 micrometer/pixel, Z: 5 micrometer/slice
XY: 0.497 micrometer/pixel, Z: 2 micrometer/slice
XY: 0.552 micrometer/pixel, Z: 1 micrometer/slice
XY: 0.662 micrometer/pixel, Z: 1 micrometer/slice
XY: 0.662 micrometer/pixel, Z: 5 micrometer/slice
T-scale
91.68 second for each time interval
91.47 second for each time interval
90.36 second for each time interval
61.16 second for each time interval
68.35 second for each time interval
61.22 second for each time interval
68.8 second for each time interval
60.47 second for each time interval
73.71 second for each time interval
61.84 second for each time interval
73.18 second for each time interval
70.99 second for each time interval
90.14 second for each time interval
60.01 second for each time interval
68.34 second for each time interval
185.34 second for each time interval
94.27 second for each time interval
61.35 second for each time interval
69.70 second for each time interval
71.7 second for each time interval
61.53 second for each time interval
68.86 second for each time interval
61.23 second for each time interval
71.07 second for each time interval
dblink
None
External links
None
Experiment type
-
Microscope type
FluorescenceMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
FV1200MPE-IX83 Olympus
Detector model
-
Objective model
UPLSAPO 30XS Olymups
Filter set
-
Biological Process
activation of MAPERK kinase
(
GO:0000186
)
,
epidermal growth factor signaling pathway
(
GO:0007173
)
,
ERBB2 signaling pathway
(
GO:0038128
)
,
ERBB2-EGFR signaling pathway
(
GO:0038134
)
Cellular Component
-
People
Contact
Masamichi Imajo
Kyoto University
Graduate School of Biostudies
Laboratory of Bioimaging and Cell Signaling
Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501 Japan
Contributors
Yu Muta, Yoshihisa Fujita, Kenta Sumiyama, Atsuro Sakurai, M. Mark Taketo, Tsutomu Chiba, Hiroshi Seno, Kazuhiro Aoki, Michiyuki Matsuda, Masamichi Imajo
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Mus muculus
(
NCBITaxon:10090
)
Living images of the small intestine of EKAREV-NLS mice before and after administration of 0.1 mg /kg body weight of TPA
Fluorescence images of the small intestine of EKAREV-NLS mice before administration of 5mg /kg body weight of MEK inhibitor(PD0325902)
Fluorescence images of the small intestine of EKAREV-NLS mice after administration of 5mg /kg body weight of MEK inhibitor(PD0325902)
Timelapse imaging of intestinal crypts of EKAREV-NLS mice
Timelapse imaging of intestinal crypts of EKAREV-NLS mice
Fluorescence images of an EKAREV-NLS organoid cultured under normal conditions
Mus muculus
(
NCBITaxon:10090
)
Timelapse imaging of ERK activity in the EKAREV-NLS organoids treated with 1000nM TPA
Mus muculus
(
NCBITaxon:10090
)
Timelapse imaging of ERK activity in the EKAREV-NLS organoids before treated with 1000nM TPA
Timelapse imaging of ERK activity in the EKAREV-NLS organoids treated with MEK inhibitor
Timelapse imaging of ERK activity in the EKAREV-NLS organoids before treated with MEK inhibitor
Timelapse imaging snapshot of ERK activity in each cell after the treatment with MEK inhibitor at time point 0
Timelapse imaging snapshot of ERK activity in each cell after the treatment with MEK inhibitor at time point 0
Timelapse imaging snapshot of ERK activity in each cell before the treatment with MEK inhibitor
Timelapse imaging snapshot of ERK activity in each cell before the treatment with MEK inhibitor
Time courses images of the average ERK activity in EKAREV-NLS organoids treated with 100nM BRAF inhibitor (SB590885)
Time courses images of the average ERK activity in EKAREV-NLS organoids treated with 100nM BRAF inhibitor (SB590885)
Time courses images of the average ERK activity in EKAREV-NLS organoids after treated with 10uM BRAF inhibitor (SB590885) at time point 0
Time courses images of the average ERK activity in EKAREV-NLS organoids before treated with 10uM BRAF inhibitor (SB590885)
Time courses images of the average ERK activity in EKAREV-NLS organoids after treated with 1uM BRAF inhibitor (SB590885) at time point 0
Time courses images of the average ERK activity in EKAREV-NLS organoids before treated with 1uM BRAF inhibitor (SB590885)
Timelapse imaging of the EKAREV-NLS organoids showing pulse-like ERK activation
Mus muculus
(
NCBITaxon:10090
)
An image of the EKAREV-NLS organoids cultured under the EGF-starved condition (NR)
Mus muculus
(
NCBITaxon:10090
)
An image of the EKAREV-NLS organoids cultured under the EGF-starved condition (NR)
Mus muculus
(
NCBITaxon:10090
)
An image of the EKAREV-NLS organoids cultured under the EGF-starved condition (NR)
An image of the EKAREV-NLS organoids cultured under the normal condition (ENR)
An image of the EKAREV-NLS organoids cultured under the normal condition (ENR)
An image of the EKAREV-NLS organoids cultured under the normal condition (ENR)
Mus muculus
(
NCBITaxon:10090
)
Timelapse imaging of the average ERK activity in the EGF-starved EKAREV-NLS organoids (NR) after stimulation with 50ng/ml EGF from time point 0 for 30minutes
Mus muculus
(
NCBITaxon:10090
)
Timelapse imaging of the average ERK activity in the EGF-starved EKAREV-NLS organoids (NR) before stimulation with 50ng/ml EGF
Mus muculus
(
NCBITaxon:10090
)
Timelapse imaging of the average ERK activity in the normal EKAREV-NLS organoids (ENR) after stimulation with 50ng/ml EGF from time point 0
Mus muculus
(
NCBITaxon:10090
)
Timelapse imaging of the average ERK activity in the normal EKAREV-NLS organoids (ENR) before stimulation with 50ng/ml EGF
Mus muculus
(
NCBITaxon:10090
)
Time courses images of ERK activity in the three representative cells
Timelapse images of organoids treated with an EGFR inhibitor (PD153035, 1 μM)
Mus muculus
(
NCBITaxon:10090
)
Timelapse images of organoids treated with an MMP inhibitor (marimastat, 100 μM)
Time courses images of EKAREV-NLS organoids treated with ErbB2i (CP-724714) from time point 0
Time courses images of EKAREV-NLS organoids before treated with ErbB2i (CP-724714)
Time courses images of EKAREV-NLS organoids treated with EGFRi (PD153035)
Time courses images of EKAREV-NLS organoids treated with EGFRi (PD153035)
Mus muculus
(
NCBITaxon:10090
)
Time courses images of EKAREV-NLS organoids treated with EGFRi (PD153035) and ErbB2i (CP-724714)
Mus muculus
(
NCBITaxon:10090
)
Time courses images of EKAREV-NLS organoids treated with EGFRi (PD153035) and ErbB2i (CP-724714)
Time courses images of average ERK activity after treatment with EGFRi in EKAREV-NLS organoids
Time courses images of average ERK activity before treatment with EGFRi in EKAREV-NLS organoids
An image of average ERK activity after treatment with ErbB2i in EKAREV-NLS organoids
An image of average ERK activity after treatment with ErbB2i in EKAREV-NLS organoids
An image of average ERK activity after treatment with ErbB2i in EKAREV-NLS organoids
An image of average ERK activity before treatment with ErbB2i in EKAREV-NLS organoids
An image of average ERK activity before treatment with ErbB2i in EKAREV-NLS organoids
An image of average ERK activity before treatment with ErbB2i in EKAREV-NLS organoids
Time courses images of average ERK activity of EGF-starved organoids (NR) treated with EGFRi and then stimulated with 50ng/ml EGF
Time courses images of average ERK activity of EGF-starved organoids (NR) treated with EGFRi before stimulated with 50ng/ml EGF